Covalent Attachment of Multilayers on Poly(tetrafluoroethylene) Surfaces

Aumsuwan, Nattharika; Ye, Sang‐Ho; Wagner, William R.; Urban, Marek W.

doi:10.1021/la201957a

Cited by 14 publications

(10 citation statements)

References 32 publications

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“…An adapted radio frequency glow discharge (RFGD) method [41] was used to form a consistent and durable negative charge on the surface of polypropylene (PP) mesh in order to facilitate the desired LbL coating. The presence of a negatively charged surface was confirmed by the appearance of two peaks at 284 eV (C-C) and 288 eV (O-C=O) on the carbon spectrum and a peak at 532 eV on the oxygen spectrum, while pristine mesh only had a peak at 284 eV (C-C) when evaluated by X-ray photoelectron spectroscopy (XPS) (Figure 2a).…”

Section: Resultsmentioning

confidence: 99%

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Shifts in macrophage phenotype at the biomaterial interface via IL-4 eluting coatings are associated with improved implant integration

et al. 2017

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The present study tests the hypothesis that transient, early-stage shifts in macrophage polarization at the tissue-implant interface from a pro-inflammatory (M1) to an anti-inflammatory/regulatory (M2) phenotype mitigates the host inflammatory reaction against a non-degradable polypropylene mesh material and improves implant integration downstream. To address this hypothesis, a nanometer-thickness coating capable of releasing IL-4 (an M2 polarizing cytokine) from an implant surface at early stages of the host response has been developed. Results of XPS, ATR-FTIR and Alcian blue staining confirmed the presence of a uniform, conformal coating consisting of chitosan and dermatan sulfate. Immunolabeling showed uniform loading of IL-4 throughout the surface of the implant. ELISA assays revealed that the amount and release time of IL-4 from coated implants were tunable based upon the number of coating bilayers and that release followed a power law dependence profile. In-vitro macrophage culture assays showed that implants coated with IL-4 promoted polarization to an M2 phenotype, demonstrating maintenance of IL-4 bioactivity following processing and sterilization. Finally, in-vivo studies showed that mice with IL-4 coated implants had increased percentages of M2 macrophages and decreased percentages of M1 macrophages at the tissue-implant interface during early stages of the host response. These changes were correlated with diminished formation of fibrotic capsule surrounding the implant and improved tissue integration downstream. The results of this study demonstrate a versatile cytokine delivery system for shifting early-stage macrophage polarization at the tissue-implant interface of a non-degradable material and suggest that modulation of the innate immune reaction at early stages of the host response may represent a preferred strategy for promoting biomaterial integration and success.

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Section: Resultsmentioning

confidence: 99%

“…An adapted radio frequency glow discharge (RFGD) based on a previously developed microwave plasma procedure was used to obtain a negatively charged surface [41]. Briefly, maleic anhydride powder (1.5 gr) was placed into a glass plate inside of the machine chamber.…”

Section: Methodsmentioning

confidence: 99%

Shifts in macrophage phenotype at the biomaterial interface via IL-4 eluting coatings are associated with improved implant integration

et al. 2017

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“…Although many efforts have been made to improve these materials for biomedical applications, their biocompatibility is still a challenge. One way to achieve biocompatibility is coating the surface with natural molecules or covalent immobilization of biomacromolecules to the activated or subsequently grafted polymer surface [10][11][12]. The surface can be activated, i.e.…”

Section: Introductionmentioning

confidence: 99%

Layer-by-layer assembly of thin organic films on PTFE activated by cold atmospheric plasma

et al. 2014

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An air diffuse coplanar surface barrier discharge is used to activate the surface of polytetrafluoroethylene (PTFE) samples, which are subsequently coated with polyvinylpyrrolidone (PVP) and tannic acid (TAN) single, bi-and multilayers, respectively, using the dipcoating method. The surfaces are characterized by X-ray Photoelectron Spectroscopy (XPS), Attenuated Total Reflection -Fourier Transform Infrared Spectroscopy (ATR-FTIR) and Atomic Force Microscopy (AFM). The XPS measurements show that with plasma treatment the F/C atomic ratio in the PTFE surface decreases, due to the diminution of the concentration of CF 2 moieties, and also oxygen incorporation through formation of new C-O, C=O and O=C-O bonds can be observed. In the case of coated samples, the new bonds indicated by XPS show the bonding between the organic layer and the surface, and thus the stability of layers, while the gradual decrease of the concentration of F atoms with the number of deposited layers proves the creation of PVP/TAN bi-and multi-layers. According to the ATR-FTIR spectra, in the case of PVP/TAN multilayer hydrogen bonding develops between the PVP and TAN, which assures the stability of the multilayer. The AFM lateral friction measurements show that the macromolecular layers homogeneously coat the plasma treated PTFE surface.

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“…ePTFE is composed of a fluorocarbon polymer, formed into sheets by extrusion. A three-layer polymer is created, with a middle microporous, elastic layer surrounded by two layers of polymer fibrils [2]. The resultant structure provides for a high strength-to-weight ratio and resistance to dilatation.…”

Section: Synthetic Biomaterialsmentioning

confidence: 99%

Biomaterial applications in cardiovascular tissue repair and regeneration

Lam

2012

Expert Review of Cardiovascular Therapy

176

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Cardiovascular disease physically damages the heart, resulting in loss of cardiac function. Medications can help alleviate symptoms, but it is more beneficial to treat the root cause by repairing injured tissues, which gives patients better outcomes. Besides heart transplants, cardiac surgeons use a variety of methods for repairing different areas of the heart such as the ventricular septal wall and valves. A multitude of biomaterials are used in the repair and replacement of impaired heart tissues. These biomaterials fall into two main categories: synthetic and natural. Synthetic materials used in cardiovascular applications include polymers and metals. Natural materials are derived from biological sources such as human donor or harvested animal tissues. A new class of composite materials has emerged to take advantage of the benefits of the strengths and minimize the weaknesses of both synthetic and natural materials. This article reviews the current and prospective applications of biomaterials in cardiovascular therapies.

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Covalent Attachment of Multilayers on Poly(tetrafluoroethylene) Surfaces

Cited by 14 publications

References 32 publications

Shifts in macrophage phenotype at the biomaterial interface via IL-4 eluting coatings are associated with improved implant integration

Shifts in macrophage phenotype at the biomaterial interface via IL-4 eluting coatings are associated with improved implant integration

Layer-by-layer assembly of thin organic films on PTFE activated by cold atmospheric plasma

Biomaterial applications in cardiovascular tissue repair and regeneration

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