Covalent Attachment of P15 Peptide to Ti Alloy Surface Modified with Polymer to Enhance Osseointegration of Implants

Abstract

Titanium (Ti) and its alloys are used in orthopedic and dental implants due to their excellent physical properties and biocompatibility. Although Ti exhibits superior osteoconductive properties compared to those of polymer-based implants, improved bone-on growth properties are required for enhanced surgical outcomes and improved recovery surgical interventions. Herein, we demonstrate a novel surface modification strategy to enhance the osteoconductivity of Ti surfaces through the grafting-from procedure of a reactive copolymer via surface-initiated atom transfer radical polymerization (SI-ATRP). Then, postpolymerization conjugation of the P15 peptide, an osteoblast binding motif, was successfully carried out. Subsequent in vitro studies revealed that the surface modification promoted osteoblast attachment on the Ti discs at 6 and 24 h. Moreover, mineral matrix deposition by osteoblasts was greater for the surface-modified Ti than for plain Ti and P15 randomly absorbed onto the Ti surface. These results suggest that the strategy for postpolymerization incorporation of P15 onto a Ti surface with a polymer interface may provide improved osseointegration outcomes, leading to enhanced quality of life for patients.

The Impact of Dental Implant Surface Modifications on Osseointegration and Biofilm Formation

“…P15 is a synthetic 15 amino acid peptide that imitates the cell-binding domain of the alpha-1 chain of human collagen [ 182 ]. Attaching this peptide to the titanium surface has been shown to promote osseointegration, particularly by increasing osteoblast and mesenchymal cell attachment, spreading, and osteogenic gene expression and differentiation [ 90 ]. Interestingly, when P15 was fused with competence-stimulating peptide (CSP), the fusion peptide displayed the osteogenic activity and suppressed biofilm formation [ 183 ].…”

Grafting polymer brushes by ATRP from functionalized poly(ether ether ketone) microparticles

“…Prior work by our group and others demonstrated the presence of a tertiary amine improved cell growth and adhesion. 33 In this and M n,GPC slightly lower than the theoretical value (Table 1, entry 4). This is plausibly due to a small portion of new chains that were generated in situ by free radicals from AIBN.…”

“…Prior work by our group and others demonstrated the presence of a tertiary amine improved cell growth and adhesion 33 . In this report, we also used PDMAEMA.…”

Facile and Versatile Surface Functional Polyetheretherketone with Enhanced Bacteriostasis and Osseointegrative Capability for Implant Application

“…Additionally, adequate osseointegration is a crucial measure to settle the problems of bone loss and implant loosening at the implant–bone interface. As reported, whether the internal bone tissue of an implant can stably osseointegrate with the implant to achieve good initial stability is the key to the implant long-term survival. , Specifically, studies have shown that BMSCs in the bone marrow cavity are recruited around the implant and differentiate into osteoblasts under the influence of different cytokines, playing an important role in promoting early implant osseointegration. , In our research, first, PEEK was sulfonated to form a three-dimensional porous structure, which is more conducive to the adhesion and proliferation of BMSCs. Second, previous research showed that lithium chloride can regulate the osteogenic differentiation of BMSCs and increase bone mass formation in the treatment of psychiatric patients.…”