Covalent binding of polyethylene glycol to the surface of red blood cells as detected and followed up by cell electrophoresis and rheological methods

Sabolović, Domagoj; Sestier, Claude; Perrotin, P.; Guillet, R.; Tefit, Maurel; Boynard, M.

doi:10.1002/(sici)1522-2683(20000101)21:2<301::aid-elps301>3.0.co;2-c

Cited by 9 publications

(1 citation statement)

References 9 publications

(12 reference statements)

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“…15 The second portion was derivatized by cyanuric-PEG-5000 (Sigma-Aldrich), incubated for one hour and subsequently washed twice. 16 The third portion was kept as a control. The derivatized and control RBCs were received three days after initial shipment and the maleimide-PEG-RBCs, cyanuric PEG-RBCs, and control RBCs were each assayed (as described below) 10 times in order to determine a baseline value of agglutination with which to compare all other results.…”

Section: Methodsmentioning

confidence: 99%

DePEGylation Studies: PEG-RBC Stability in Conditions Consistent with Massive Transfusion

et al. 2011

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Each year the United States population receives an estimated 12 to 14 million units of packed red blood cells (RBCs) and whole blood. 1,2 It is estimated that 33% of transfusions associated with trauma are with unmatched type O RBCs (UORBC). 3 UORBCs have been proven effective and relatively safe 3 however, by masking RBC surface antigens the risk of transfusion reaction may be further decreased. It is, therefore, important to evaluate and validate the stability of antigen masked RBCs, which may play a part in avoiding transfusion reactions. These antigen-masked RBCs would be regularly subjected to abnormal in vivo conditions commonly associated with massive transfusion such as lactic acidosis, bacteremia, and in vitro irradiation, which is frequently used to sterilize and decrease T Lymphocyte counts in RBC units before transfusion. This study compared two methods of masking RBC antigens by PEGylation: maleimide-PEGylation and cyanuric chloride-PEGylation. RBC PEGylation effectively masks the Rh(D) antigen 4,5 and PEG-RBC bond stability was evaluated by comparison of pre and post exposure agglutination with anti-D sera. While the stability of maleimide-PEG-RBCs remained unaffected, the cyanuric chloride-PEG-RBCs remained stable in the bacteremia and irradiation studies, but critical concentrations of lactic acid caused dePEGylation. Further studies are warranted to ensure in vivo stability.

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Section: Methodsmentioning

confidence: 99%

DePEGylation Studies: PEG-RBC Stability in Conditions Consistent with Massive Transfusion

et al. 2011

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Electrokinetic properties of noncharged lipid nanocapsules: Influence of the dipolar distribution at the interface

et al. 2005

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Lipid nanocapsules (LNCs) containing poly(ethylene glycol) (PEG) were developed according to a phase inversion process without organic solvent. The distribution of PEG chains at the surface was determined due to electrokinetic properties, in order to correlate it with protein adsorption potentiality. In this aim, electrophoretic mobilities were measured as a function of ionic strength and pH, for particles differing by their size, dialysis effects, and the presence or not of lecithin in their shell. The study allowed the determination of the isoelectric point (pI) as well as the charge density (ZN) in relation with the dipolar distribution in the polyelectrolyte accessible layer (depth = 1/lambda), by using soft-particle electrophoresis analysis. These parameters pointed out that the PEG surface organization was dependent on the particle size. Moreover, this organization could be modified by dialyzing particles and/or by formulating them with or without lecithin. Lecithin was found to be present in the inner part of the polyelectrolyte layer and to play a role in the outer part disorganization. Dialyzing LNCs formulated with lecithin allowed to obtain stable and well-structured nanocapsules, ready to an in vivo use as drug delivery system.

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Thiolation Mediated Pegylation Platform to Generate Functional Universal Red Blood Cells

Nacharaju

Manjula

Acharya

2007

Artificial Cells, Blood Substitutes, and Biotechnology

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The PEGylation that adds an extension arm on protein amino groups with the conservation of their positive charge masks the A and D antigens of erythrocytes efficiently. In the present study, the efficiency of masking the antigens of RBC by PEGylation protocols that do not conserve the charge with and without adding extension arms is compared. The conjugation of PEG-5000 to RBCs through the addition of extension arms masked the D antigen more efficiently than the other protocol. A combination of PEG-5 K and PEG-20 K is needed to mask the A antigen, irrespective of the PEGylation approach. The oxygen affinity of the PEGylated RBCs increased by the extension arm facilitated PEGylation. The protocol involving the conjugation of PEG-chains without adding extension arm did not alter the oxygen affinity of RBCs. A combination of PEGylation protocols is an alternate strategy to generate universal red blood cells with good levels of oxygen affinity.

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Covalent binding of polyethylene glycol to the surface of red blood cells as detected and followed up by cell electrophoresis and rheological methods

Cited by 9 publications

References 9 publications

DePEGylation Studies: PEG-RBC Stability in Conditions Consistent with Massive Transfusion

DePEGylation Studies: PEG-RBC Stability in Conditions Consistent with Massive Transfusion

Electrokinetic properties of noncharged lipid nanocapsules: Influence of the dipolar distribution at the interface

Thiolation Mediated Pegylation Platform to Generate Functional Universal Red Blood Cells

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