Covalent capture and electrochemical quantification of pathogenic <i>E. coli</i>

Klass, Sarah H.; Sofen, Laura E.; Hallberg, Zachary F.; Fiala, Tahoe A.; Ramsey, Alexandra V.; Dolan, Nicholas S.; Francis, Matthew B.; Furst, Ariel L.

doi:10.1039/d0cc08420d

Cited by 15 publications

(14 citation statements)

References 26 publications

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“…However, clever detection schemes combined with new platform technologies could help to decrease the time for detection and increase specificity. My group, for instance, works on developing electrochemical sensors to detect infectious disease agents by harnessing the inherent activity of microbes to capture them on an electrode surface (Fig 2; Klass et al , 2021); improving electrodes for detection by decreasing their cost and difficulty of manufacture (Zamani et al , 2021c); and incorporating biological amplification to increase selectivity and sensitivity (Zamani et al , 2021b). Our ultimate goal is to improve global health equity by decreasing the financial barriers to testing (Sofen & Furst, 2020; Castle et al , 2021; Zamani et al , 2021b).…”

Section: New Applications For Electrochemical Biosensorsmentioning

confidence: 99%

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Electricity, chemistry and biomarkers: an elegant and simple package

Zamani

Furst

2022

EMBO Reports

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Section: New Applications For Electrochemical Biosensorsmentioning

confidence: 99%

“…We developed an electrochemical sensor to detect E. coli from complex food samples and body fluids by covalent capture of the microbes at the electrode surface (Klass et al , 2021). The overall workflow involves the addition of a synthetic amino acid to a sample.…”

Section: New Applications For Electrochemical Biosensorsmentioning

confidence: 99%

Electricity, chemistry and biomarkers: an elegant and simple package

Zamani

Furst

2022

EMBO Reports

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“…Furthermore, biosensors have been developed to identify viruses and bacteria. Electrochemical biosensors utilize changes in electrical properties to determine the presence or absence of an analyte of interest (Figure B,C). − The detection of intact viruses could provide a simple way to test multiple specimens without extensive sample processing. Using an optofluidic-nanoplasmonic sensor, Yanik et al demonstrated the use of plasmonic nanoholes and pathogen-specific antibodies to gather measurable transmission information to identify viral loads.…”

Section: Detection Of Known and Unknown Pathogensmentioning

confidence: 99%

Early Warning Diagnostics for Emerging Infectious Diseases in Developing into Late-Stage Pandemics

et al. 2021

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Conspectus The spread of infectious diseases due to travel and trade can be seen throughout history, whether from early settlers or traveling businessmen. Increased globalization has allowed infectious diseases to quickly spread to different parts of the world and cause widespread infection. Posthoc analysis of more recent outbreaksSARS, MERS, swine flu, and COVID-19has demonstrated that the causative viruses were circulating through populations for days or weeks before they were first detected, allowing disease to spread before quarantines, contact tracing, and travel restrictions could be implemented. Earlier detection of future novel pathogens could decrease the time before countermeasures are enacted. In this Account, we examined a variety of novel technologies from the past 10 years that may allow for earlier detection of infectious diseases. We have arranged these technologies chronologically from prehuman predictive technologies to population-level screening tools. The earliest detection methods utilize artificial intelligence to analyze factors such as climate variation and zoonotic spillover as well as specific species and geographies to identify where the infection risk is high. Artificial intelligence can also be used to monitor health records, social media, and various publicly available data to identify disease outbreaks faster than traditional epidemiology. Secondary to predictive measures is monitoring infection in specific sentinel animal species, where domestic animals or wildlife are indicators of potential disease hotspots. These hotspots inform public health officials about geographic areas where infection risk in humans is high. Further along the timeline, once the disease has begun to infect humans, wastewater epidemiology can be used for unbiased sampling of large populations. This method has already been shown to precede spikes in COVID-19 diagnoses by 1 to 2 weeks. As total infections increase in humans, bioaerosol sampling in high-traffic areas can be used for disease monitoring, such as within an airport. Finally, as disease spreads more quickly between humans, rapid diagnostic technologies such as lateral flow assays and nucleic acid amplification become very important. Minimally invasive point-of-care methods can allow for quick adoption and use within a population. These individual diagnostic methods then transfer to higher-throughput methods for more intensive population screening as an infection spreads. There are many promising early warning technologies being developed. However, no single technology listed herein will prevent every future outbreak. A combination of technologies from across our infection timeline would offer the most benefit in preventing future widespread disease outbreaks and pandemics.

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“…Recently, a functional electrochemical sensor was reported to detect pathogenic strains of Escherichia coli , 37 which are the most common cause of urinary tract infections (UTIs) 38 , 39 and are a major contributor to foodborne illnesses. 40 – 43 Antibody- and aptamer-based electrochemical sensors have been reported to monitor these pathogens, with detection limits as low as 10 colony-forming units (CFU)/mL.…”

Section: Detecting Emerging Pathogens Electrochemicallymentioning

confidence: 99%

Bioelectrochemical platforms to study and detect emerging pathogens

et al. 2021

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Graphic Abstract The ongoing SARS-CoV-2 pandemic has emphasized the importance of technologies to rapidly detect emerging pathogens and understand their interactions with hosts. Platforms based on the combination of biological recognition and electrochemical signal transduction, generally termed bioelectrochemical platforms, offer unique opportunities to both sense and study pathogens. Improved bio-based materials have enabled enhanced control over the biotic–abiotic interface in these systems. These improvements have generated platforms with the capability to elucidate biological function rather than simply detect targets. This advantage is a key feature of recent bioelectrochemical platforms applied to infectious disease. Here, we describe developments in materials for bioelectrochemical platforms to study and detect emerging pathogens. The incorporation of host membrane material into electrochemical devices has provided unparalleled insights into the interaction between viruses and host cells, and new capture methods have enabled the specific detection of bacterial pathogens, such as those that cause secondary infections with SARS-CoV-2. As these devices continue to improve through the merging of hi-tech materials and biomaterials, the scalability and commercial viability of these devices will similarly improve.

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Covalent capture and electrochemical quantification of pathogenic E. coli

Abstract: Pathogenic E. coli pose a significant threat to public health by causing both foodborne illness and urinary tract infections. A sensitive electrochemical method to detect these pathogens can be used for surveillance and to prevent illness.

Cited by 15 publications

References 26 publications

Electricity, chemistry and biomarkers: an elegant and simple package

Electricity, chemistry and biomarkers: an elegant and simple package

Early Warning Diagnostics for Emerging Infectious Diseases in Developing into Late-Stage Pandemics

Bioelectrochemical platforms to study and detect emerging pathogens

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