Abstract:Nanotechnology is one of the fastest growing technologies in this era. Gold nanoparticle (AuNP) based immunoassays have been performed on the basis of antigen-antibody interaction using AuNP antibody conjugates. Lateral flow immunoassays(LFA) which are also based on AuNP antibody conjugates are useful innovation in nanotechnology and widely applied in medicine and research fields. However, there are some limitations of the present LFA kits such as sensitivity and stability. In the study, we showed the result o… Show more
“…In parallel with this procedure, the citrates capped on the surfaces of Au NRs were exchanged with HS–PEG–COOH. Then, preparation of the Au NR–TiO 2 nanocomplexes (Au NR–TiO 2 NCs) was performed by coupling of the two surface-modified nanomaterials to lead the formation of the amide bond between the amine group of APTES–TiO 2 NPs and the carboxyl group of PEG–Au NRs [37, 38]. Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Next, the purchased citrates-capped Au NRs were exchanged with HS–PEG–COOH to prepare the PEG–Au NRs. Finally, the preparation of the Au NR–TiO 2 NCs was performed by coupling of the two surface-modified nanomaterials (APTES–TiO 2 NPs and PEG–Au NRs) to lead to the formation of the amide bond between the amine group of APTES and the carboxyl group of PEG [37, 38].…”
BackgroundRecently, a combination of photodynamic therapy (PDT) and photothermal therapy (PTT) to generate reactive oxygen species (ROS) and heat to kill cancer cells, respectively has attracted considerable attention because it gives synergistic effects on the cancer treatment by utilizing the radiation of nontoxic low-energy photons such as long wavelength visible light and near IR (NIR) penetrating into subcutaneous region. For the effective combination of the phototherapies, various organic photosensitizer-conjugated gold nanocomplexes have been developed, but they have still some disadvantages due to photobleaching and unnecessary energy transfer of the organic photosensitizers.ResultsIn this study, we fabricated novel inorganic phototherapeutic nanocomplexes (Au NR–TiO2 NCs) by conjugating gold nanorods (Au NRs) with defective TiO2 nanoparticle clusters (d-TiO2 NP clusters) and characterized their optical and photothermal properties. They were observed to absorb a broad range of visible light and near IR (NIR) from 500 to 1000 nm, exhibiting the generation of ROS as well as the photothermal effect for the simultaneous application of PDT and PTT. The resultant combination of PDT and PTT treatments of HeLa cells incubated with the nanocomplexes caused a synergistic increase in the cell death compared to the single treatment.ConclusionThe higher efficacy of cell death by the combination of PDT and PTT treatments with the nanocomplexes is likely attributed to the increases of ROS generation from the TiO2 NCs with the aid of local surface plasma resonance (LSPR)-induced hot electrons and heat generation from Au NRs, suggesting that Au NR–TiO2 NCs are promising nanomaterials for the in vivo combinatorial phototherapy of cancer.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0432-4) contains supplementary material, which is available to authorized users.
“…In parallel with this procedure, the citrates capped on the surfaces of Au NRs were exchanged with HS–PEG–COOH. Then, preparation of the Au NR–TiO 2 nanocomplexes (Au NR–TiO 2 NCs) was performed by coupling of the two surface-modified nanomaterials to lead the formation of the amide bond between the amine group of APTES–TiO 2 NPs and the carboxyl group of PEG–Au NRs [37, 38]. Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Next, the purchased citrates-capped Au NRs were exchanged with HS–PEG–COOH to prepare the PEG–Au NRs. Finally, the preparation of the Au NR–TiO 2 NCs was performed by coupling of the two surface-modified nanomaterials (APTES–TiO 2 NPs and PEG–Au NRs) to lead to the formation of the amide bond between the amine group of APTES and the carboxyl group of PEG [37, 38].…”
BackgroundRecently, a combination of photodynamic therapy (PDT) and photothermal therapy (PTT) to generate reactive oxygen species (ROS) and heat to kill cancer cells, respectively has attracted considerable attention because it gives synergistic effects on the cancer treatment by utilizing the radiation of nontoxic low-energy photons such as long wavelength visible light and near IR (NIR) penetrating into subcutaneous region. For the effective combination of the phototherapies, various organic photosensitizer-conjugated gold nanocomplexes have been developed, but they have still some disadvantages due to photobleaching and unnecessary energy transfer of the organic photosensitizers.ResultsIn this study, we fabricated novel inorganic phototherapeutic nanocomplexes (Au NR–TiO2 NCs) by conjugating gold nanorods (Au NRs) with defective TiO2 nanoparticle clusters (d-TiO2 NP clusters) and characterized their optical and photothermal properties. They were observed to absorb a broad range of visible light and near IR (NIR) from 500 to 1000 nm, exhibiting the generation of ROS as well as the photothermal effect for the simultaneous application of PDT and PTT. The resultant combination of PDT and PTT treatments of HeLa cells incubated with the nanocomplexes caused a synergistic increase in the cell death compared to the single treatment.ConclusionThe higher efficacy of cell death by the combination of PDT and PTT treatments with the nanocomplexes is likely attributed to the increases of ROS generation from the TiO2 NCs with the aid of local surface plasma resonance (LSPR)-induced hot electrons and heat generation from Au NRs, suggesting that Au NR–TiO2 NCs are promising nanomaterials for the in vivo combinatorial phototherapy of cancer.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0432-4) contains supplementary material, which is available to authorized users.
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