Covalent coupling of polysaccharides to silicon and silicon rubber surfaces

Abstract

A method to produce a hydrogel by covalently coupling dextran polysaccharide to silicon and silicon rubber surfaces is described. A bifunctional epoxysilane was used as coupling mediator and the conditions for the reaction were optimized. Coupling of the silane to silicon rubber was possible only after oxidation of the surface by plasma etching. The reaction between the epoxy-group and dextran was concentration dependent and required high concentrations of dextran. The turnover rate of fibrinogen in solution at the hydrophilic surface was found to be high when measured by ellipsometry.

A quantitative and selective chromatography method for determining coverages of multiple proteins on surfaces

“…Dextrans are known to show very low nonspecific interactions with proteins [37] and dextran coatings reduce protein adsorption at surfaces enhancing biocompatibility [38][39][40]. For the first time, this study shows that BSA preferentially absorbs to dextran, relative to Fg.…”

Fast Transient Cytokine–Receptor Interactions Monitored in Real Time by Reflectometric Interference Spectroscopy

“…Second, silica chemistry quickly decomposes in alkaline environment, and therefore acidic to neutral reaction conditions are required. Direct coupling of dextran to surfaces activated with epoxy-functional silanes under neutral conditions is difficult to control and yields low dextran loadings (26,27) (and unpublished results). For more efficient coupling, we used dextrans functionalized with amine groups.…”

Biomimetic Dextran Coatings On Silicon Wafers: Thin Film Properties And Wetting

“…The thickness of the polysaccharide coatings was determined using an index of refraction n=1.5 [6]. Measurements were performed at five different spots on a given sample.…”