Covalent functionalization of graphene sheets for plasmid DNA delivery: experimental and theoretical study

Assali, Mohyeddin; Kittana, Naim; Badran, Ismail; Omari, Safa

doi:10.1039/d3ra00727h

Cited by 4 publications

(6 citation statements)

References 60 publications

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“…Copyright 2020 Elsevier B.V. D. The designed covalent functionalization of graphene sheets with tetramine linker. Reproduced with permission from ref . Copyright 2023 The Royal Society of Chemistry.…”

Section: Delivery System For Nonviral Gene Therapymentioning

confidence: 99%

“…The disadvantage of these substances is that they are very incompatible and insoluble in aqueous environments, which makes their application for biological use somewhat complicated. 108 Recently, Assali et al 134 developed a covalently functionalized graphene sheet as a gene delivery vehicle (Figure 6D). They used a derivative of tetraethylene glycol connected to polyamine groups to enhance the water dispersibility and the ability of the graphene sheet to interact with DNA, transfected into tumor cells for expression with great potential for exploitation.…”

Section: Nonmetal Nanoparticlesmentioning

confidence: 99%

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DNA-Based Nonviral Gene Therapy─Challenging but Promising

Guan,

Pei,

Song

2024

Mol. Pharmaceutics

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Over the past decades, significant progress has been made in utilizing nucleic acids, including DNA and RNA molecules, for therapeutic purposes. For DNA molecules, although various DNA delivery systems have been established, viral vector systems are the go-to choice for large-scale commercial applications. However, viral systems have certain disadvantages such as immune response, limited payload capacity, insertional mutagenesis and pre-existing immunity. In contrast, nonviral systems are less immunogenic, not size limited, safer, and easier for manufacturing compared with viral systems. What's more, nonviral DNA vectors have demonstrated their capacity to mediate specific protein expression in vivo for diverse therapeutic objectives containing a wide range of diseases such as cancer, rare diseases, neurodegenerative diseases, and infectious diseases, yielding promising therapeutic outcomes. However, exogenous plasmid DNA is prone to degrade and has poor immunogenicity in vivo. Thus, various strategies have been developed: (i) designing novel plasmids with special structures, (ii) optimizing plasmid sequences for higher expression, and (iii) developing more efficient nonviral DNA delivery systems. Based on these strategies, many interesting clinical results have been reported. This Review discusses the development of DNA-based nonviral gene therapy, including novel plasmids, nonviral delivery systems, clinical advances, and prospects. These developments hold great potential for enhancing the efficacy and safety of nonviral gene therapy and expanding its applications in the treatment of various diseases.

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Section: Delivery System For Nonviral Gene Therapymentioning

confidence: 99%

Section: Nonmetal Nanoparticlesmentioning

confidence: 99%

DNA-Based Nonviral Gene Therapy─Challenging but Promising

Guan,

Pei,

Song

2024

Mol. Pharmaceutics

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“… 262 Covalent functionalization improves the water dispersibility and stability of graphene derivates. 262 , 284 , 285 Carboxylic acids or hydrophilic polymers are used the most. Carboxylic treatment is simple, and attachment of carboxyl, epoxy, and hydroxyl side groups on graphene leads to its increased dispersibility in the aqueous phase.…”

Section: Use Of Graphene-based Materials To Enhance Pdtmentioning

confidence: 99%

“…262 Covalent functionalization improves the water dispersibility and stability of graphene derivates. 262,284,285 Carboxylic acids or https://doi.org/10.2147/IJN.S461300…”

Section: Use Of Graphene-based Materials To Enhance Pdt Functionaliza...mentioning

confidence: 99%

Graphene-Based Photodynamic Therapy and Overcoming Cancer Resistance Mechanisms: A Comprehensive Review

Dilenko,

Bartoň Tománková,

Válková

et al. 2024

IJN

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“…Because of the damage caused by the current treatment used, researchers resorted to the use of targeted cancer therapy based on nanomaterials taking advantage of enhanced permeability and retention phenomena and the overexpression of specific receptors in cancer cells. 4,5 One of these well-developed nanomaterials is carbon-based nanomaterials such as carbon nanotubes (CNTs), 6,7 graphene, 8 and carbon nanodots (CNDs). 9…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of fluorescent carbon nanodots from sage leaves for selective anticancer activity on 2D liver cancer cells and 3D multicellular tumor spheroids

Sawalha,

Abdallah,

Barham

et al. 2023

Nanoscale Adv.

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Covalent functionalization of graphene sheets for plasmid DNA delivery: experimental and theoretical study

Abstract: Graphene sheets were functionalized covalently with an appropriate linker as a novel vector for p-DNA delivery into mammalian cells. The transfected cancer cells produced green fluorescence as an indicator of successful gene delivery.

Cited by 4 publications

References 60 publications

DNA-Based Nonviral Gene Therapy─Challenging but Promising

DNA-Based Nonviral Gene Therapy─Challenging but Promising

Graphene-Based Photodynamic Therapy and Overcoming Cancer Resistance Mechanisms: A Comprehensive Review

Green synthesis of fluorescent carbon nanodots from sage leaves for selective anticancer activity on 2D liver cancer cells and 3D multicellular tumor spheroids

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