Covalent Immobilization of &amp;#945;-Glucosidase Enzyme onto Amine Functionalized Multi-walled Carbon Nanotubes

Mohiuddin, M.; Arbain, Dachyar; Islam, A.K.M. Shafiqul; Ahmad, M. S.; Ahmad, Mohammad Norazmi

doi:10.2174/1573413710666140116213526

Cited by 17 publications

(11 citation statements)

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“…Twenty grams of St and 0.5 g of AA were then added, and the temperature was maintained at 70 °C. After that, about 0.2 g of APS was added into 10 mL of deionised water followed by pouring into the formulation in the three-neck flask to initiate the radical polymerisation reaction, and the polymerisation process was proceeded for 8 h. The as-synthesised carboxyl latex spheres were harvested by centrifugation with deionised water twice at 13,000 rpm for 20 min [ 23 , 27 , 28 ] and re-suspended in deionised water at room temperature (25 °C) until use. The morphology and average size of the PSA latex particles were determined by scanning electron microscopy (SEM).…”

Section: Methodsmentioning

confidence: 99%

“…Kawde and Wang [ 29 ] attached the PSA latex particles to a DNA reporter probe to be used as DNA hybridisation label via loading of streptavidin-coated latex particles with biotin-coated AuNPs. Kuan et al [ 24 , 27 ] and Liew et al [ 24 , 27 ] also reported the same avidin-biotin binding method using gold-PSA-DNA probe conjugates. Pinijsuwan et al [ 28 ] reported the use of electrostatic method for the loading of PSA particles attached to DNA reporter probe and the polyelectrolyte gold-coated PSA particles were used as a label for hybridisation that amplified the DPV current response.…”

Section: Introductionmentioning

confidence: 94%

“…Latex-gold nanoparticle has been previously employed as the DNA hybridisation label via avidin/biotin binding to the DNA probe in the detection of V. cholerae [ 24 ], fish pathogen Aphanomyces invadans [ 27 ], E. coli [ 28 ], and nonspecific DNA hybridisation [ 29 ], whereby the latex spheres were coated with a multilayer of polyelectrolyte before the negatively charged colloids of gold nanoparticles were electrostatically attached thereunto. Kawde and Wang [ 29 ] attached the PSA latex particles to a DNA reporter probe to be used as DNA hybridisation label via loading of streptavidin-coated latex particles with biotin-coated AuNPs.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres

Heng¹,

Futra

Ling³

2017

Nanoscale Res Lett

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An ultrasensitive electrochemical biosensor for the determination of pathogenic Vibrio cholerae (V. cholerae) DNA was developed based on polystyrene-co-acrylic acid (PSA) latex nanospheres-gold nanoparticles composite (PSA-AuNPs) DNA carrier matrix. Differential pulse voltammetry (DPV) using an electroactive anthraquninone oligonucleotide label was used for measuring the biosensor response. Loading of gold nanoparticles (AuNPs) on the DNA-latex particle electrode has significantly amplified the faradaic current of DNA hybridisation. Together with the use of a reported probe, the biosensor has demonstrated high sensitivity. The DNA biosensor yielded a reproducible and wide linear response range to target DNA from 1.0 × 10−21 to 1.0 × 10−8 M (relative standard deviation, RSD = 4.5%, n = 5) with a limit of detection (LOD) of 1.0 × 10−21 M (R 2 = 0.99). The biosensor obtained satisfactory recovery values between 91 and 109% (n = 3) for the detection of V. cholerae DNA in spiked samples and could be reused for six consecutive DNA assays with a repeatability RSD value of 5% (n = 5). The electrochemical biosensor response was stable and maintainable at 95% of its original response up to 58 days of storage period.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres

Heng¹,

Futra

Ling³

2017

Nanoscale Res Lett

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“…The spectra were recorded in the wave number range of 500–4000 cm −1 using 1:20 ratios of sample and potassium bromide (KBr) in the preparation of pellets. Scanning electron microscope (SEM) was used for characterization of immobilized AG enzyme on MWCNTs [ 22 ]. The colorimetric measurements were performed using a Lambda 25 UV–VIS spectrometer (PerkinElmer, USA).…”

Section: Methodsmentioning

confidence: 99%

Alpha-Glucosidase Enzyme Biosensor for the Electrochemical Measurement of Antidiabetic Potential of Medicinal Plants

Mohiuddin¹,

Arbain

Islam

et al. 2016

Nanoscale Res Lett

Self Cite

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A biosensor for measuring the antidiabetic potential of medicinal plants was developed by covalent immobilization of α-glucosidase (AG) enzyme onto amine-functionalized multi-walled carbon nanotubes (MWCNTs-NH2). The immobilized enzyme was entrapped in freeze-thawed polyvinyl alcohol (PVA) together with p-nitrophenyl-α-d-glucopyranoside (PNPG) on the screen-printed carbon electrode at low pH to prevent the premature reaction between PNPG and AG enzyme. The enzymatic reaction within the biosensor is inhibited by bioactive compounds in the medicinal plant extracts. The capability of medicinal plants to inhibit the AG enzyme on the electrode correlates to the potential of the medicinal plants to inhibit the production of glucose from the carbohydrate in the human body. Thus, the inhibition indicates the antidiabetic potential of the medicinal plants. The performance of the biosensor was evaluated to measure the antidiabetic potential of three medicinal plants such as Tebengau (Ehretis laevis), Cemumar (Micromelum pubescens), and Kedondong (Spondias dulcis) and acarbose (commercial antidiabetic drug) via cyclic voltammetry, amperometry, and spectrophotometry. The cyclic voltammetry (CV) response for the inhibition of the AG enzyme activity by Tebengau plant extracts showed a linear relation in the range from 0.423–8.29 μA, and the inhibition detection limit was 0.253 μA. The biosensor exhibited good sensitivity (0.422 μA/mg Tebengau plant extracts) and rapid response (22 s). The biosensor retains approximately 82.16 % of its initial activity even after 30 days of storage at 4 °C.

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“…The many different supports commonly used in the immobilization of enzymes include inorganic oxides such as SiO 2 (Kolodziejczak-Radzimska 2017; Jin et al 2018), TiO 2 (Zivkovic et al 2016;Haghighi et al 2017) and ZrO 2 (Masuda et al 2014), minerals such as bentonite (Cengiz et al 2012) and halloysite (Zhai et al 2010;Chao et al 2013), and carbon materials (Mohiuddin et al 2014;Wu et al 2017;Das et al 2018). These materials have been used in the immobilization of lipase (Cai et al 2016;Zdarta et al 2015;Kolodziejczak-Radzimska et al 2018b;Heater et al 2018), cysteine (Xiao et al 2010;Noori et al 2016), urease (Piccinini et al 2017;Tak et al 2017), α-amylase (Demkina et al 2017, tyrosinase (Abdollahi et al 2017), laccase (Pogorilyi et al 2017) and other enzymes.…”

Section: Introductionmentioning

confidence: 99%

A novel biocatalytic system obtained via immobilization of aminoacylase onto sol–gel derived ZrO2·SiO2 binary oxide material: physicochemical characteristic and catalytic activity study

Kołodziejczak-Radzimska

Ciesielczyk

Jesionowski

2019

Adsorption

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Oxide material of a new type, ZrO 2 ·SiO 2 (raw and carbonyl-grafted), was used as support in the immobilization of aminoacylase from Aspergillus melleus. The ZrO 2 ·SiO 2 was synthesized via sol-gel method. The obtained material was additionally modified with glutaraldehyde. Various physicochemical analyses were used to confirm the effectiveness of the modification and immobilization processes, including Fourier transform infrared spectroscopy, laser Doppler velocimetry and lowtemperature N 2 sorption. The immobilization process was performed within 3 h using different concentrations of enzyme solution (1, 3, 5 and 7 mg/mL), and the Bradford method was used to determine the quantity of immobilized enzyme. The resulting biocatalytic systems were then used as catalysts in the hydrolysis of different N-acetyl-dl-amino acids (leading to l-methionine, l-cysteine, l-serine and l-tryptophan). Based on this reaction the apparent and relative catalytic activities were determined. The highest activity of the immobilized enzyme was attained in the synthesis of l-methionine (the apparent activities of aminoacylase immobilized on raw and carbonyl-grafted ZrO 2 ·SiO 2 were 4112 and 4947 U/g, respectively). Furthermore, the effect of pH and temperature on catalytic activity, as well as the storage stability and reusability of the prepared biocatalytic systems were determined. Aminoacylase immobilized on carbonyl-grafted ZrO 2 ·SiO 2 retains 85% of its initial activity after 30 days of storage and 70% after five reaction cycles.

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Covalent Immobilization of α-Glucosidase Enzyme onto Amine Functionalized Multi-walled Carbon Nanotubes

Cited by 17 publications

References 0 publications

Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres

Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres

Alpha-Glucosidase Enzyme Biosensor for the Electrochemical Measurement of Antidiabetic Potential of Medicinal Plants

A novel biocatalytic system obtained via immobilization of aminoacylase onto sol–gel derived ZrO2·SiO2 binary oxide material: physicochemical characteristic and catalytic activity study

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