Covalent immobilization of cholesterol esterase and cholesterol oxidase on polyaniline films for application to cholesterol biosensor

Singh, Suman; Solanki, Pratima R.; Pandey, Manoj Kumar; Malhotra, Bansi D.

doi:10.1016/j.aca.2005.10.008

Cited by 124 publications

(77 citation statements)

References 21 publications

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“…58,59 Singh et al 58 have attempted to modify the electrochemically deposited PANI films using cholesterol oxidase (CHOx), cholesterol esterase (ChEt), and HRP enzymes and investigated their optical and electrochemical biosensing response, toward total cholesterol, following the pathways 4 and 5 shown in Figure 4. The designed bioelectrodes showed wide detection range, decent sensitivity, and reasonable stability; however, there is enough scope for improvement in the response time and the apparent Michaelis-Menten constant (K m app ) parameter (indicative of enzyme-analyte interactions) of these bioelectrodes.…”

Section: Polyaniline-based Cholesterol Biosensorsmentioning

confidence: 99%

Polyaniline-based biosensors

Malhotra¹,

Dhand²,

Lakshminarayanan³

et al. 2015

NDD

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Biosensors are the subject of an immensely growing field of research owing to their broad range of applications in medicines, pharmacy, environmental monitoring, food and process control, defense and security, and principally in diagnostics. Diverse materials have been investigated for the advancement of biosensors in terms of their miniaturization, sophistication, cost, biosensing features, ie, detection limit, sensitivity, stability, selectivity, etc. Polyaniline (PANI) is one of the most interesting conductive polymers for biosensor design in view of its excellent electrochemical properties (polyelectrocatalytic characteristics, reversible redox behavior, and electrochemical tunability), straightforward processability, long-term environmental stability, and functionality-rich chemical structure. In this review, an attempt is made to compile almost all the existent literature on PANI-based biosensors in terms of enzymatic biosensors (for H 2 O 2 , glucose, cholesterol, phenol/polyphenol/catecholamine detection), genosensors (DNA sensing), and immunosensors from 2006 to 2015. Furthermore, reports available on the biosensing of urea, uric acid, creatinine, pesticides, amino acids, and other clinically significant analytes are also assembled to provide a comprehensive overview on PANI-based biosensors.

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Section: Polyaniline-based Cholesterol Biosensorsmentioning

confidence: 99%

Polyaniline-based biosensors

Malhotra¹,

Dhand²,

Lakshminarayanan³

et al. 2015

NDD

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“…2). Electrochemical transducers have been effectively utilized for the estimation of cholesterol in the system (Charpentier & Murr, 1995;Singh et al, 2006;Zhou et al, 2006;Arya et al, 2007). Based on number and reliability of optical methods, a variety of optical transducers have been employed for cholesterol sensing, namely monitoring: luminescence, change in color of dye, fluorescence and others (Arya et al, 2008).…”

Section: Biosensors In Cardiovascular Diseasementioning

confidence: 99%

Biosensors for health applications

Gouvêa¹

2011

Biosensors for Health, Environment and Biosecurity

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“…This result is in good agreement with that of the polymer based cholesterol biosensors. 17,19,21,36 The pH value has important effects on enzyme activity and thus enzyme activity should change with the pH of buffer. The sensitivities of Pt/PoPD/ChOx enzyme electrode as function of pH are shown in Figure 4.…”

Section: Effects Of Buffer Concentration and Phmentioning

confidence: 99%

“…Unlike various methods that used in development a biosensor, electropolymerization presents many advantages such as reproducibility, thickness control and the uniformity of the polymer film and give permission to simple preparation of polymer film modified electrodes. 32 The conducting polymers such as polypyrrole, 3,[33][34][35] polyaniline 17,36 and polythiophene 37 have been widely used for the biosensors development. These polymers have a high conductivity, stability and also amount of biomaterial can be controlled during electropolymerization process.…”

Section: (1)mentioning

confidence: 99%

Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

Zeybek¹,

Zeybek²,

Pekmez³

et al. 2012

J. Braz. Chem. Soc.

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Um eletrodo enzimático amperométrico baseado em polímero foi preparado para detecção amperométrica de colesterol livre. Inicialmente, o filme polimérico poli(o-fenilenodiamina) (PoPD) foi eletropolimerizado no meio acetonitrila-água contendo o monômero o-fenilenodiamina (oPD) e ácido canforsulfônico em eletrodo de Pt através da técnica de voltametria cíclica. A enzima oxidase colesterol foi imobilizada sobre a superfície do eletrodo Pt/PoPD. A determinação de colesterol foi realizada por maio do monitoramento da corrente de oxidação de H 2 O 2 produzido enzimaticamente a +0,7 V vs. Ag/AgCl. A concentração da solução tampão, o valor de pH e a temperatura de trabalho otimizados foram 0,05 mol L −1 , 7,5 and 40 °C, respectivamente. O intervalo de trabalho do eletrodo de enzima para colesterol foi 9,8 × 10 −3 -11 µmol L −1 e tempo de resposta 150 s. Os efeitos de possíveis interferências presentes em amostras de soro em resposta ao eletrodo de enzima foram examinados. A determinação do colesterol total em amostras de soro foi realizada usando o eletrodo de enzima Pt/PoPD/ChOx proposto e os resultados foram em bom acordo com os obtidos pelo método espectrofotométrico.A polymer-based amperometric enzyme electrode (Pt/PoPD/ChOx) was prepared for the amperometric detection of free cholesterol. Firstly, poly(o-phenylenediamine) (PoPD) polymer film was prepared in acetonitrile-water medium containing o-phenylenediamine (oPD) monomer and (±)-10-camphorsulfonic acid (HCSA) on Pt electrode by the use of cyclic voltammetry technique. Cholesterol oxidase (ChOx) enzyme was immobilized onto Pt/PoPD electrode surface. The determination of cholesterol was performed via monitoring of the oxidation current of enzymatically produced H 2 O 2 at +0.7 V vs. Ag/AgCl. Optimum buffer concentration, pH and working temperature were found as 0.05 mol L −1 , 7.5 and 40 °C, respectively. The working range of enzyme electrode to cholesterol was 9.8 × 10 −3 -11 µmol L −1 and response time 150 s. The effects of possible interferences present in serum samples on response of enzyme electrode were examined. The determination of total cholesterol in serum samples was performed by using proposed Pt/PoPD/ChOx enzyme electrode and results were in good agreement with those obtained by spectrophotometric method.

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Covalent immobilization of cholesterol esterase and cholesterol oxidase on polyaniline films for application to cholesterol biosensor

Cited by 124 publications

References 21 publications

Polyaniline-based biosensors

Polyaniline-based biosensors

Biosensors for health applications

Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

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