Covalent immobilization of delipidated human serum albumin on poly(pyrrole-2-carboxylic) acid film for the impedimetric detection of perfluorooctanoic acid

Moro, Giulia; Bottari, Fabio; Liberi, S.; Covaceuszach, Sonia; Cassetta, Alberto; Angelini, Alessandro; Wael, Karolien De; Moretto, Ligia Maria

doi:10.1016/j.bioelechem.2020.107540

Cited by 18 publications

(12 citation statements)

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“…The elucidation of the molecular basis of the interaction between PFOA and hSA is expected to have implications for the development of superior hSA-based molecular receptors for diagnostic and biotechnological applications. 24 2 | RESULTS 2.1 | Overview of the crystal structure of hSA in complex with PFOA and myristic acid…”

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Unveiling the binding mode of perfluorooctanoic acid to human serum albumin

et al. 2021

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Perfluorooctanoic acid (PFOA) is a toxic compound that is absorbed and distributed throughout the body by noncovalent binding to serum proteins such as human serum albumin (hSA). Though the interaction between PFOA and hSA has been already assessed using various analytical techniques, a high resolution and detailed analysis of the binding mode is still lacking. We report here the crystal structure of hSA in complex with PFOA and a medium-chain saturated fatty acid (FA). A total of eight distinct binding sites, four occupied by PFOAs and four by FAs, have been identified. In solution binding studies confirmed the 4:1 PFOA-hSA stoichiometry and revealed the presence of one high and three low affinity binding sites. Competition experiments with known hSA-binding drugs allowed locating the high affinity binding site in subdomain IIIA. The elucidation of the molecular basis of the interaction between PFOA and hSA might provide not only a better assessment of the absorption and elimination mechanisms of these compounds in vivo but also have implications for the development of novel molecular receptors for diagnostic and biotechnological applications. K E Y W O R D S binding mode, crystal structure, fluoroalkyl substances, human serum albumin, molecular interaction, perfluorooctanoic acid 1 | INTRODUCTION Perfluorooctanoic acid (PFOA) is a perfluoroalkyl substance (PFAS) with a carboxyl functional group and seven fluorinated carbon atoms. 1 PFOA is a man-made

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Unveiling the binding mode of perfluorooctanoic acid to human serum albumin

et al. 2021

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“…The peak current at +0.983 V decreased with increasing CV cycles, indicating that the polymer layer was successfully generated on the electrode surface. 48–51 As seen in Fig. 2(B), AuNPs coated on the ITO substrate had high peak currents, and these high peak currents illustrated that the AuNPs on the electrode surface accelerated the electron transfer kinetics of the redox probe.…”

Section: Resultsmentioning

confidence: 88%

Label-free and reagent-less electrochemical detection of nucleocapsid protein of SARS-CoV-2: an ultrasensitive and disposable biosensor

Aydın

Sezgintürk

2022

New J. Chem.

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“…The PFOS biosensor presented a wide range of linear detection between 5 and 500 nmol/L with a lowest detection limit of LOD = 1.6 nmol/L and high selectivity over different chemicals that co-exist with PFOS in micro-polluted water, which suggested that this biosensor might be implemented in real water monitoring. Moreover, an impedimetric biosensor for the detection of perfluorooctanoic acid (PFOA) was designed by the modification of a graphite screen printed electrodes (G-SPE) (Moro et al, 2020). This electrochemical biosensor was developed by the modification of a G-SPE with a covalently immobilized human serum albumin (hSA) as bioreceptor into pyrrole-2-carboxylic acid (Py-2-COOH) and PFOA was detected in range between 100 nmol/L and 5 μmol/ L. In addition, some other bioreceptors have been applied for the detection and monitoring of PFAs, such as DNA immobilized into Au/(Peroxide polypyrrole) electrode, which was applied for the detection of PFOA (Lu et al, 2011).…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Detection and Tertiary Treatment Technologies of Poly-and Perfluoroalkyl Substances in Wastewater Treatment Plants

Araújo

Rodríguez-Hernández

González-González

et al. 2022

Front. Environ. Sci.

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PFAS are a very diverse group of anthropogenic chemicals used in various consumer and industrial products. The properties that characterize are their low degradability as well as their resistance to water, oil and heat. This results in their high persistence in the environment and bioaccumulation in different organisms, causing many adverse effects on the environment as well as in human health. Some of their effects remain unknown to this day. As there are thousands of registered PFAS, it is difficult to apply traditional technologies for an efficient removal and detection for all. This has made it difficult for wastewater treatment plants to remove or degrade PFAS before discharging the effluents into the environment. Also, monitoring these contaminants depends mostly on chromatography-based methods, which require expensive equipment and consumables, making it difficult to detect PFAS in the environment. The detection of PFAS in the environment, and the development of technologies to be implemented in tertiary treatment of wastewater treatment plants are topics of high concern. This study focuses on analyzing and discussing the mechanisms of occurrence, migration, transformation, and fate of PFAS in the environment, as well the main adverse effects in the environment and human health. The following work reviews the recent advances in the development of PFAS detection technologies (biosensors, electrochemical sensors, microfluidic devices), and removal/degradation methods (electrochemical degradation, enzymatic transformation, advanced oxidation, photocatalytic degradation). Understanding the risks to public health and identifying the routes of production, transportation, exposure to PFAS is extremely important to implement regulations for the detection and removal of PFAS in wastewater and the environment.

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Covalent immobilization of delipidated human serum albumin on poly(pyrrole-2-carboxylic) acid film for the impedimetric detection of perfluorooctanoic acid

Abstract: TitleCovalent immobilization of delipidated human serum albumin on poly(pyrrole-2carboxylic) acid film for the impedimetric detection of perfluorooctanoic acid Article typeResearch Paper

Cited by 18 publications

References 54 publications

Unveiling the binding mode of perfluorooctanoic acid to human serum albumin

Unveiling the binding mode of perfluorooctanoic acid to human serum albumin

Label-free and reagent-less electrochemical detection of nucleocapsid protein of SARS-CoV-2: an ultrasensitive and disposable biosensor

Detection and Tertiary Treatment Technologies of Poly-and Perfluoroalkyl Substances in Wastewater Treatment Plants

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