Covalent Immobilization of Transglucosidase onto Polymer Beads for Production of Isomaltooligosaccharides

Mendis, Mihiri; Mendoza, Bridget R.; Şimşek, Şenay

doi:10.1007/s10562-012-0866-5

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…Therefore, the ratio of transglycosylation to hydrolysis first increases and then decreases. It was observed that the IMO yield depends on the relative rates of transglycosylation and hydrolysis reactions [4,23]. In this study, when the IMO yield reached the peak value, the percentage of isomaltose and isomaltotriose was 16.36 and 8.73% (w/w), respectively, in the biphasic medium, which showed higher levels than that in the aqueous medium (13.33 and 7.49%, w/w) ( Table 2).…”

Section: Imo Formation Kinetics In Optimum Conditionsmentioning

confidence: 49%

“…IMO are attracting considerable interest in the food industry because of their bifidus-stimulating activity, low caloric value, and low cariogenic properties [2,3]. At present, IMO are manufactured through enzymatic processes, wherein an a-glucosidase from Aspergillus niger catalyzes transglycosylation to the 6-OH of the accepting glucose unit, resulting in formation of new (1,6)oligosaccharides such as isomaltose, panose, and isomaltotriose [4].…”

Section: Introductionmentioning

confidence: 99%

“…Immobilized enzymes have been used in the food, pharmaceutical, and other industrial bioprocesses to not only eliminate potential protein contamination from the product but also to reuse the enzyme and hence reduce operational costs [4,5]. Various immobilization techniques have been reported in the literatures, including adsorption, entrapment, and chemical interactions (either covalent or non-covalent) between the enzyme and the support [5].…”

Section: Introductionmentioning

confidence: 99%

“…Epoxy (oxirane)-activated resins, such as Eupergit C or Sepabeads, are among the most widely used supports for enzyme covalent binding [5]. In a recent study, transglucosidase immobilized onto Amberzyme oxirane resin improved the catalytic activity and the stability toward pH and temperature [4].…”

Section: Introductionmentioning

confidence: 99%

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Improved synthesis of isomaltooligosaccharides using immobilized α-glucosidase in organic–aqueous media

Wang

Niu

et al. 2017

Food Sci Biotechnol

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α-Glucosidase was immobilized onto an epoxy-activated resin (Eupergit C) to catalyze maltose into isomaltooligosaccharides (IMO), and then the effects of organic-aqueous media on the enzymatic properties of immobilized α-glucosidase were examined. An immobilization efficiency of 79.61% was obtained under the condition of pH 6.0, ionic strength of 2.0 M, and 30 mg of protein/g of resin. The butyl acetate-aqueous biphasic system was found to significantly improve the catalytic activity of the immobilized enzyme and the yield of IMO. The highest yield of IMO (50.83%, w/w) was obtained at pH 4.5 and 55 °C in a butyl acetate/buffer system (25:75, v/v). In addition, the immobilized enzyme particles were distributed into the organic phase after the completion of transglycosylation, which facilitates the separation and recycling use of the immobilized enzyme. Immobilized α-glucosidase retains a robust reusability in this continuous operation model. The present findings are of potential in improving the IMO manufacturing process.

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Section: Imo Formation Kinetics In Optimum Conditionsmentioning

confidence: 49%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improved synthesis of isomaltooligosaccharides using immobilized α-glucosidase in organic–aqueous media

Wang

Niu

et al. 2017

Food Sci Biotechnol

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Genome Shuffling of Aspergillus niger for Improving Transglycosylation Activity

Chen

et al. 2013

Appl Biochem Biotechnol

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Isomaltooligosaccharides (IMO), the glucosylsaccharides used as food additives, are made from saccharified starch by enzymes or microbial cells with transglycosylation activity. This study aimed to generate shuffled futants of Aspergillus niger with enhanced transglycosylation activity for industrial IMO production. The starting mutant population was generated by (60)Co-γ radiation; mutants with higher transglycosylation activity were selected and subjected to recursive protoplast fusion. The resulting fusants were screened by a novel high-throughput method based on detecting non-fermentable reducing sugar. After three rounds of genome shuffling, the best performing strain GS3-3 was obtained, its transglycosylation activity (14.91 U/mL) was increased by 194.1 % compared to that of original strain C-6181. In fermentor test, transglycosylation activity of GS3-3 was obtained at 16.61 U/mL. The mycelia of GS3-3 were reused ten times to produce IMO syrup from liquefied cassava starch containing about 280 g/L total sugar within 4 days. The conversion of liquefied cassava starch to IMO was at 71.3-72.1 %, which was higher than the best conversion (68 %) ever reported. GS3-3 shows a great potential for industrial IMO production.

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Continuous Production of Isomalto-oligosaccharides by Thermo-inactivated Cells of Aspergillus niger J2 with Coarse Perlite as an Immobilizing Material

Huang

et al. 2018

Appl Biochem Biotechnol

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The coarse perlite 40-80 mesh was selected as an immobilizing material and put into a packed bed reactor (PBR) to continuously convert maltose to isomalto-oligosaccharides (IMOs). The PBR was prepared by mixing the thermo-inactivated cells (TIC) from Aspergillus niger J2 strain with the coarse perlite, then the mixture was put into an overpressure-resistant column. Compared with diatomite 40-80 mesh and thin perlite 80-120 mesh in PBR, coarse perlite was chosen as the best filtration aid, when the ratio of coarse perlite versus TIC was 1:1. The thermal and pH stability of the free and immobilized TIC and the optimum conditions for the transglycosylation reactions were determined. The results show that approximately 75 and 82% and 87 and 91% of α-glucosidase activity were reserved for free and immobilized TIC at temperatures from 30 to 60 °C and pH from 3.00 to 7.00 for 12 h, respectively. With 30% malt syrup under the conditions of 50 °C and pH 4.00, a mini-scale packed bed reactor (Mi-PBR) and medium-scale packed bed reactor (Me-PBR) could continuously produce IMO over 25 and 34 days with the yield of effective IMO (eIMO) ≥ 35% and total IMO (tIMO) ≥ 50%, respectively. The strategy of mixing the coarse perlite with TIC in PBR is a novel approach to continuously produce IMO and has great application potential in industry.

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Covalent Immobilization of Transglucosidase onto Polymer Beads for Production of Isomaltooligosaccharides

Cited by 5 publications

References 27 publications

Improved synthesis of isomaltooligosaccharides using immobilized α-glucosidase in organic–aqueous media

Improved synthesis of isomaltooligosaccharides using immobilized α-glucosidase in organic–aqueous media

Genome Shuffling of Aspergillus niger for Improving Transglycosylation Activity

Continuous Production of Isomalto-oligosaccharides by Thermo-inactivated Cells of Aspergillus niger J2 with Coarse Perlite as an Immobilizing Material

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