Covalent Immobilization of Unilamellar Liposomes in Gel Beads for Chromatography

Liposome Immobilization on Cross-linked Polymer Gel by In Situ Formation of Cleavable Covalent Bonds

“…Sandberg et al first reported immobilization of liposomes on the type of gel particles [4]. The work was extended by Yang et al to immobilization of proteoliposomes [5] and further to chromatographic systems [6][7][8] in investigation of drug partitioning to lipid bilayer membrane [9][10][11] and protein-membrane interaction [12]. These studies clearly demonstrate usefulness of liposome-gel conjugates.…”

Preparation of Hollow Fiber Immobilized Liposome Membrane

“…At the same time, the above immobilized liposome techniques can also be applied for the stress-mediated mutual separation of proteins 20) and for the protein refolding column 1) . In these immobilized liposome matrixes, the liposome was immobilized via (a) physical entrapment method 24) , (b) antigen-antibody method 25) , (c) hydrophobic ligand method 26) , (d) covalent binding method 27) and so on. Although these immobilization methods can be utilized for the bioseparation uses, there are some problems such as the capacity of immobilized liposome, the stability of the immobilized liposome, difficulty or non-scalability of the column operation and so on.…”

“…Membrane module, consisted of hollow fiber membranes, has been produced in some membrane companies and utilized in the many research areas 29) .…”

Separation of compounds interacting with liposome membrane in combined prescription of traditional Chinese medicines with immobilized liposome chromatography

“…Liposomes [10][11][12] formed by phosphatidylcholine (PC), the main components found in cell membrane, or unilaminar phospholipids [13][14][15][16], were non-covalently or covalently immobilized on soft gel or porous silica stationary phases to probe the penetration ability of compounds through biological membranes, which has been considered as important parameters to evaluate their bioactivity [17,18]. Initial studies indicated that a good correlation between ILC and intestinal mucosa model was obtained for a series of drugs [19][20][21][22]. Previously, we investigated the application of ILC in separation and analysis of permeable components in single Chinese herb [23].…”