Covalent Labeling of Nucleosides with VIII‐ and VI‐Valent Osmium Complexes

Trefulka, Mojmír; Ostatná, Veronika; Havran, Luděk; Fojta, Miroslav; Paleček, Emil

doi:10.1002/elan.200703848

Cited by 49 publications

(53 citation statements)

References 37 publications

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“…This is much better than the 1-electron electrode process of Fc. Perhaps the highest electron yield can be obtained with Os(VIII)bipy-NA adducts producing electrochemical responses due to the catalytic hydrogen evolution at mercury and solid amalgam electrodes ( [114] and references therein). Such electrode process allows determination of nucleic acids at subpicomolar concentrations [182].…”

Section: Labeling Of Nucleic Acidsmentioning

confidence: 99%

Fifty Years of Nucleic Acid Electrochemistry

Paleček

2009

Electroanalysis

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Electrochemistry of nucleic acids (NAs) is a booming field offering sensors for DNA hybridization and DNA damage. Since the middle of the 1990s, when the development of the DNA sensors began, the number of papers in this field is steadily increasing. Electroactivity of DNA and RNA was reported for the first time in 1958. Using oscillographic polarography at controlled AC (OP, AC chronopotentiometry) and a dropping mercury electrode it was shown within several years that OP signals respond to changes in DNA structure and can be used to study DNA denaturation, renaturation, premelting and DNA damage. During the first three decades a number of important findings were done, including introductions of covalently bound electroactive labels in DNA, DNA-modified electrodes, application of solid electrodes, etc., which are now used in the development of the NA sensors. On the other hand a lot of knowledge obtained in the first three decades has remained largely unknown to the present generation of researchers.

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Section: Labeling Of Nucleic Acidsmentioning

confidence: 99%

Fifty Years of Nucleic Acid Electrochemistry

Paleček

2009

Electroanalysis

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“…More than 30 years ago solid electrodes were applied in nucleic acid analysis and oxidation of adenine and guanine residues in DNA and RNA at carbon electrodes was demonstrated [7][8][9][10][11]. First covalently bound electroactive labels were introduced into DNA in the beginning of the 1980s [12,13] and are currently used to end-label nucleic acids in the DNA hybridization sensors [14][15][16][17]. Labeling of nucleic acids is very useful in the electrochemical analysis of nucleic acids [11,[18][19][20][21][22] but label-free DNA sensors are preferred whenever possible and convenient [11,23,24].…”

Section: Introductionmentioning

confidence: 99%

Square Wave Stripping Voltammetry of Unlabeled Single‐ and Double‐Stranded DNAs

Bartošík

Paleček

2011

Electroanalysis

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We show that, in difference to previously applied electrochemical methods working with stationary electrodes, square wave voltammetry produces well-developed peaks II SW (specific for dsDNA) and III SW yielded by ssDNA at hanging mercury drop electrode (HMDE) and solid amalgam electrodes (SAEs). Using these peaks various kinds of DNA structural transitions can be studied, including unwinding of dsDNA at negatively charged electrode surfaces. The sensitivity of the DNA analysis is much better than that obtained with guanine oxidation signals at carbon electrodes. Both carbon electrodes and SAEs appear attractive as transducers in label-free RNA and DNA sensors.

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“…For example, osmium complexes with nitrogen ligands have been shown to bind covalently to the pyrimidim bases [19] or sugar residues [20] and subsequently used as redox indicators in electrochemical detection of DNA [21,22]. While, the element of osmium was relatively expensive and the synthesis processes for the osmium complex were inconvenient [21,22].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Studies on the Recognition of a Copper Complex to Single‐ and Double‐Stranded DNA and Its Application in Gene Biosensor

2008

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A mixed-ligands copper complex [Cu(phendione)(DAP)]SO 4 (phendione ¼ 1,10-phenanthroline-5,6-dione, DAP ¼ 2,3-diaminophenazine) was synthesized. Cyclic voltammetry showed that the complex underwent an obvious decrease of redox peak currents and positive shift of formal potential after interaction with double-stranded DNA (dsDNA), suggesting that the copper complex behaved as a typical metallointercalator for dsDNA, The recognition properties of the copper complex to single-stranded DNA (ssDNA) and dsDNA were assessed using surface-based electrochemical methods and the results suggested that the complex had obviously different redox signals at ssDNA and dsDNA modified electrodes. The copper complex was further used as an electroactive indicator for the detection of cauliflower mosaic virus (CaMV) 35S promoter gene.

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Covalent Labeling of Nucleosides with VIII‐ and VI‐Valent Osmium Complexes

Cited by 49 publications

References 37 publications

Fifty Years of Nucleic Acid Electrochemistry

Fifty Years of Nucleic Acid Electrochemistry

Square Wave Stripping Voltammetry of Unlabeled Single‐ and Double‐Stranded DNAs

Voltammetric Studies on the Recognition of a Copper Complex to Single‐ and Double‐Stranded DNA and Its Application in Gene Biosensor

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