Covalent Modifications of Albumin Cys34 as a Biomarker of Mild Oxidative Stress

Aldini, Giancarlo; Yeum, Kyung‐Jin; Vistoli, Giulio

doi:10.1002/9780813814438.ch14

Cited by 3 publications

(7 citation statements)

References 46 publications

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“…We focused on Cys34, not only because it efficiently scavenges small reactive electrophiles [13][14] , but also because its oxidation promotes a host of covalent modifications to circulating thiols 15 that can act as redox switches in homeostatic processes [16][17] . These Cys34 sulfoxidation products and disulfides represent potential biomarkers of oxidative stress and the redox state of the serum over the 1-month residence time of HSA (21-d half-life) [18][19] .…”

Section: Introductionmentioning

confidence: 99%

“…16,17 These Cys34 sulfoxidation products and disulfides represent potential biomarkers of oxidative stress and the redox state of the serum over the 1-month residence time of HSA (21 day half-life). 18,19 We initially characterized adductomes with archived serum/ plasma from healthy smokers and nonsmokers and discovered several adducts that were significantly associated with smoking. 12 Some associations had been expected, including higher levels of adducts of ethylene oxide, acrylonitrile and methylation in smokers.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Our laboratory has developed an untargeted “adductomics” method to investigate modifications at a highly nucleophilic cysteine residue (Cys34) of human serum albumin (HSA) . We focused on Cys34, not only because it efficiently scavenges small reactive electrophiles, , but also because its oxidation promotes a host of covalent modifications to circulating thiols that can act as redox switches in homeostatic processes. , These Cys34 sulfoxidation products and disulfides represent potential biomarkers of oxidative stress and the redox state of the serum over the 1-month residence time of HSA (21 day half-life). , …”

Section: Introductionmentioning

confidence: 99%

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Cys34 Adductomes Differ between Patients with Chronic Lung or Heart Disease and Healthy Controls in Central London

Liu

Grigoryan

Edmands

et al. 2018

Environ. Sci. Technol.

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Oxidative stress generates reactive species that modify proteins, deplete antioxidant defenses, and contribute to chronic obstructive pulmonary disease (COPD) and ischemic heart disease (IHD). To determine whether protein modifications differ between COPD or IHD patients and healthy subjects, we performed untargeted analysis of adducts at the Cys34 locus of human serum albumin (HSA). Biospecimens were obtained from nonsmoking participants from London, U.K., including healthy subjects (n = 20) and patients with COPD (n = 20) or IHD (n = 10). Serum samples were digested with trypsin and analyzed by liquid chromatography-high resolution mass spectrometry. Effects of air pollution on adduct levels were also investigated based on estimated residential exposures to PM, O and NO. For the 39 adducts with sufficient data, levels were essentially identical in blood samples collected from the same subjects on two consecutive days, consistent with the 28 day residence time of HSA. Multivariate linear regression revealed 21 significant associations, mainly with the underlying diseases but also with air-pollution exposures (p-value < 0.05). Interestingly, most of the associations indicated that adduct levels decreased with the presence of disease or increased pollutant concentrations. Negative associations of COPD and IHD with the Cys34 disulfide of glutathione and two Cys34 sulfoxidations, were consistent with previous results from smoking and nonsmoking volunteers and nonsmoking women exposed to indoor combustion of coal and wood.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cys34 Adductomes Differ between Patients with Chronic Lung or Heart Disease and Healthy Controls in Central London

Liu

Grigoryan

Edmands

et al. 2018

Environ. Sci. Technol.

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“…20 These Cys34 disulfides represent potential biomarkers of the redox state of the serum over the 1-month residence time of HSA. 21, 22 …”

Section: Introductionmentioning

confidence: 99%

“…Oxidation of Cys34 to the reactive sulfenic acid (Cys34-SOH) can lead to formation of mixed Cys34-disulfides from reactions between Cys34-SOH and circulating low-molecular-weight thiols . These Cys34 disulfides represent potential biomarkers of the redox state of the serum over the 1 month residence time of HSA. , …”

Section: Introductionmentioning

confidence: 99%

Profiling the Serum Albumin Cys34 Adductome of Solid Fuel Users in Xuanwei and Fuyuan, China

Grigoryan

Edmands

et al. 2016

Environ. Sci. Technol.

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Xuanwei and Fuyuan counties in China have the highest lung cancer rates in the world due to household air pollution from combustion of smoky coal for cooking and heating. To discover potential biomarkers of indoor combustion products, we profiled adducts at the Cys34 locus of human serum albumin (HSA) in 29 nonsmoking Xuanwei and Fuyuan females who used smoky coal, smokeless coal or wood, and 10 local controls who used electricity or gas fuel. Our untargeted ‘adductomics’ method detected 50 tryptic peptides of HSA, containing Cys34 and prominent post-translational modifications. Putative adducts included Cys34 oxidation products, mixed disulfides, rearrangements and truncations. The most significant differences in adduct levels across fuel types were observed for S-glutathione (S-GSH) and S-γ-glutamylcysteine (S-γ-GluCys), both of which were present at lower levels in subjects exposed to combustion products than in controls. After adjustment for age and personal measurements of airborne benzo(a)pyrene, the largest reductions in levels of S-GSH and S-γ-GluCys relative to controls were observed for users of smoky coal, compared to users of smokeless coal and wood. These results point to possible depletion of GSH, an essential antioxidant, and its precursor γ-GluCys in nonsmoking females exposed to indoor-combustion products in Xuanwei and Fuyuan, China.

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Dry food affects the oxidative/antioxidant profile of dogs

Suarez

Rojano

Duque³

et al. 2023

Veterinary Medicine & Sci

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Background Including adequate concentrations of antioxidants in dog diets has been recommended to reduce their vulnerability to the action of free radicals and reactive oxygen species (ROS). Oxidative stress in dogs has been associated with a wide range of diseases and disorders, as well as with ageing. There are few reports about the influence of diet on dog's antioxidant profile and oxidative stress. Objective The objective of this study was to evaluate the effect of four types of dry dog food on the oxidative/antioxidant profile of dogs. Methods Six Beagle dog males were used. The study included four experimental diets (dry foods A–D). Each dry food was supplied for 5 weeks to all dogs, for a total of 24 weeks, including an adaptation week between one food and another. For each dry dog food, the total phenolic content (TPC), total antioxidant capacity (TAC) and cytotoxicity were evaluated. Each week, a blood sample was collected to measure ROS and TAC of plasma. A crossover repeated measures design was used. Mixed models were adjusted, and means were compared using the Tukey test. Results Food A had the highest values for TPC and TAC. Food C had the lowest levels of ROS, whereas food B had the highest TAC in the blood plasma. The dog had a significant influence on the redox state of its blood plasma, even when the same dog was fed the different dry foods. Conclusion Dry dog food influences the oxidative/antioxidant profile of dog's blood plasma; however, this seems to be unrelated to the antioxidant profile of the food.

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Covalent Modifications of Albumin Cys34 as a Biomarker of Mild Oxidative Stress

Cited by 3 publications

References 46 publications

Cys34 Adductomes Differ between Patients with Chronic Lung or Heart Disease and Healthy Controls in Central London

Cys34 Adductomes Differ between Patients with Chronic Lung or Heart Disease and Healthy Controls in Central London

Profiling the Serum Albumin Cys34 Adductome of Solid Fuel Users in Xuanwei and Fuyuan, China

Dry food affects the oxidative/antioxidant profile of dogs

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