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Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells

“…We rationalised ˙OH radicals generated by ultrasound would selectively remove the DHB unit and lead to changes in their corresponding fluorescent emissions therefore allowing us to monitor the ultrasound-induced ˙OH production in solution and in mammalian cells. 21,22…”

“…We rationalised OH radicals generated by ultrasound would selectively remove the DHB unit and lead to changes in their corresponding fluorescent emissions therefore allowing us to monitor the ultrasound-induced OH production in solution and in mammalian cells. 21,22 First, to attach the DHB unit onto each fluorophore, we synthesised a DHB-based alkylating reagent (see ESI † for full synthetic procedures and Schemes S1-S3). In brief, literature reported protocols were followed to isolate bis-silyl protected 3,5-dihydroxybenzyl alcohol 2.…”