Cover Image, Volume 28, Issue 7

Pavlova, Ina; Shipley, Shannon C.; Lanio, Marcos; Hen, René; Denny, Christine A.

doi:10.1002/hipo.23000

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, brain-wide engram mapping is far from being a standard procedure as the optimization obstacles regarding optical clearing are still a major concern and require tremendous trial-and-error efforts. For example, Pavlova et al (2018) used ArcCreERT2 × ChR2-eYFP mice to study contextual fear memory engrams in a brain-wide manner. They tested all leading clearing protocols at the time to find the ideal compatibility of wholebrain co-labeling and engram tagging combined with cFos or Arc IHC.…”

Section: Tissue Clearing For Brain-wide Engram Mappingmentioning

confidence: 99%

Tissue clearing applications in memory engram research

Yip,

Gräff

2023

Front. Behav. Neurosci.

View full text Add to dashboard Cite

A memory engram is thought to be the physical substrate of the memory trace within the brain, which is generally depicted as a neuronal ensemble activated by learning to fire together during encoding and retrieval. It has been postulated that engram cell ensembles are functionally interconnected across multiple brain regions to store a single memory as an “engram complex”, but visualizing this engram complex across the whole brain has for long been hindered by technical limitations. With the recent development of tissue clearing techniques, advanced light-sheet microscopy, and automated 3D image analysis, it has now become possible to generate a brain-wide map of engram cells and thereby to visualize the “engram complex”. In this review, we first provide a comprehensive summary of brain-wide engram mapping studies to date. We then compile a guide on implementing the optimal tissue clearing technique for engram tagging approaches, paying particular attention to visualize engram reactivation as a critical mnemonic property, for which whole-brain multiplexed immunostaining becomes a challenging prerequisite. Finally, we highlight the potential of tissue clearing to simultaneously shed light on both the circuit connectivity and molecular underpinnings of engram cells in a single snapshot. In doing so, novel brain regions and circuits can be identified for subsequent functional manipulation, thus providing an opportunity to robustly examine the “engram complex” underlying memory storage.

show abstract

Section: Tissue Clearing For Brain-wide Engram Mappingmentioning

confidence: 99%

Tissue clearing applications in memory engram research

Yip,

Gräff

2023

Front. Behav. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Several tissue clearing techniques have been developed for biomedical imaging in three dimensions. An example is X-CLARITY TM (Logos Biosystems), which optically clears tissues for subsequent fluorescence labeling of thick tissues or, in some cases, entire organs of interest [7–9]. Combined with light-sheet microscopy, structural mapping of an entire animal mouse brain has been achieved.…”

Section: Introductionmentioning

confidence: 99%