Cover Picture: Macromol. Biosci. 1/2007

Siemieniewicz, Krzysztof W.; Kajla, Mayur K.; Schrempf, Hildgund

doi:10.1002/mabi.200790000

Cited by 1 publication

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“…9 In addition, an elucidation of the biosynthesis of chitin filaments and their configuration with specific proteins was attempted by electron microscopy. 10 The characterization of (aminoethyl)-chitin/DNA nanoparticle for gene delivery was also examined. 11 Carbon-paste electrodes (CPEs) have had many applications: for use in electrocatalysis for nicotinamide adenine dinucleotide, 12 in the electroanalysis of tryptophan 13 and as a L-glutamate biosensor.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Behaviors of Wheat-Germ Agglutinin on a Chitin-modified Carbon-Paste Electrode

et al. 2008

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The voltammetric behavior of wheat-germ agglutinin (WGA) on a chitin-modified carbon-paste electrode (CPE) was investigated using glucose labeled with an electroactive compound. WGA usually consists of two subunits, each with two binding sites for sugars. WGA was immobilized on the electrode surface by selective binding to a N-acetylglucosamine residue of chitin. Because glucose also combines with WGA, the glucose was coupled with electroactive daunomycin to evaluate the binding. When a WGA-labeled glucose complex was formed, the electroactive moiety became electroinactive. The binding caused a decrease in the peak current of the labeled glucose. In a measurement of only daunomycin used as a label, the peak current in a solution with WGA was similar to that in a solution without WGA. Therefore, it is clear that the labeled glucose was held in the remaining binding site of WGA on the electrode surface. Thus, a CPE modified with chitin would be powerful as a reaction field between sugar and lectin.

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