Cover Picture: Photoreduction of Shewanella oneidensis Extracellular Cytochromes by Organic Chromophores and Dye‐Sensitized TiO₂ (ChemBioChem 24/2016)

Abstract

The cover picture shows the photoreduction of electrically conductive proteins spanning a bacterial outer membrane. The authors show that abiotic and cell‐secreted photosensitizers drive the photocatalytic reduction of extracellular cytochromes. Their studies suggest how photoenergized electrons may be delivered to the interior of non‐photosynthetic micro‐organisms for the synthesis of solar chemicals. More information can be found in the full paper by L. J. C. Jeuken, E. Reisner, J. N. Butt et al. on page 2324 in Issue 24, 2016 (DOI: 10.1002/cbic.201600339).

Membrane-spanning electron transfer proteins from electrogenic bacteria: Production and investigation

“…2Bii, typically yields 15 mg MtrC per 1L culture and enabled resolution of this protein's crystal structure by X-ray diffraction (Edwards et al, 2015). LC-MS of recently prepared protein revealed multiple sites of C-terminal cleavage complicating other studies including protein film electrochemistry (Ainsworth et al, 2016;Hwang et al, 2017). We now routinely prepare soluble MtrC Strp from a construct pJvW001 encoding for a 10 residue C-terminal extension where a two-residue spacer is followed by the Strep II tag Fig.…”