LoRa technology, renowned for its low-power, long-range capabilities in IoT applications, faces challenges in real-world scenarios, including fading channels, interference, and environmental obstacles. This paper aims to study the reliability of LoRa in Non-Line-of-Sight (NLoS) conditions and in noisy and mobile environments for Industrial IoT (IIoT) applications. Experimental measurements consider factors like vegetation and infrastructure, introducing mobility to replicate NLoS conditions. Utilizing an open-source LoRa Physical Layer (PHY) Software-Defined Radio (SDR) prototype developed with GNU Radio, we assess communication reliability through metrics such as Block Error Rate (BLER), Signal-to-Noise-Interference-plus-Noise Ratio (SINR), and data rate. The study reveals the estimated overall reliability of the LoRa signal at 90.23%, emphasizing specific configuration details. This work contributes to the broader field of LoRa communication, encompassing hardware, software, protocols, and management, enhancing our understanding of LoRa’s dependability in challenging IIoT environments.