COVID-19: A challenge for electrochemical biosensors

Kudr, Jiří; Michálek, Petr; Ilieva, Lada; Adam, Vojtěch; Zítka, Ondřej

doi:10.1016/j.trac.2021.116192

Cited by 95 publications

(83 citation statements)

References 93 publications

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“…In Brazil, specifically, the development of these devices based on the national sample of patients appears as an essential and necessary opportunity for the development of national technology with low cost and accessibility, in order to minimize the bringing down effect on the health system. Hence, developing novel electrochemical biosensors based on the detection of antigen-antibody interactions or membrane proteins of SARS-CoV-2 appears as an adequate and accessible alternative of test devices to those based on reverse transcription-polymerase chain reaction (RT-PCR) to supply the world’s current demand [ 3 , 4 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor

et al. 2021

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Due to the SARS-CoV-2 pandemic, there has been an increase in the search for affordable healthcare devices for mass testing and rapid diagnosis. In this context, this work described a new methodology for SARS-CoV-2 detection based on an impedimetric immunosensor developed using the advantageous immobilization of antibodies in the reduced graphene oxide (rGO). The rGO was obtained by chemical synthesis from the commercial graphene oxide (GO), and the materials were morphologically, electrochemically and visually characterized. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were used to evaluate the fabrication steps of the immunosensor. The electrochemical immunoassay was considered for SARS-CoV-2 spike protein RBD detection using a impedimetric immunosensor and redox couple ([(Fe(CN)6)]3−/4−) as a probe. The immunosensor was effectively developed and applied in the detection of SARS-CoV-2 spike protein RBD in saliva samples.

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Section: Introductionmentioning

confidence: 99%

A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor

et al. 2021

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“…In this conjecture, electrochemical DNA biosensors represent an alternative approach to detect viral nucleic acids, contributing to the development of point-of-care-testing methods. Genetic screening devices are considered an ideal strategy for the COVID-19 diagnosis, particularly in the first to second week after infection when anti-SARS-CoV-2 antibodies cannot be detected (immune window) [30] . Table 1 summarizes the main electrochemical DNA biosensors reported in the literature for the SARS-CoV-2 detection.…”

Section: Resultsmentioning

confidence: 99%

Nanostructured sensor platform based on organic polymer conjugated to metallic nanoparticle for the impedimetric detection of SARS-CoV-2 at various stages of viral infection

Avelino

Santos

Frías

et al. 2021

Journal of Pharmaceutical and Biomedical Analysis

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The projection of new biosensing technologies for genetic identification of SARS-COV-2 is essential in the face of a pandemic scenario. For this reason, the current research aims to develop a label-free flexible biodevice applicable to COVID-19. A nanostructured platform made of polypyrrole (PPy) and gold nanoparticles (GNP) was designed for interfacing the electrochemical signal in miniaturized electrodes of tin-doped indium oxide (ITO). Oligonucleotide primer was chemically immobilized on the flexible transducers for the biorecognition of the nucleocapsid protein (N) gene. Methodological protocols based on cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and atomic force microscopy (AFM) were used to characterize the nanotechnological apparatus. The biosensor’s electrochemical performance was evaluated using the SARS-CoV-2 genome and biological samples of cDNA from patients infected with retrovirus at various disease stages. It is inferred that the analytical tool was able to distinguish the expression of SARS-CoV-2 in patients diagnosed with COVID-19 in the early, intermediate and late stages. The biosensor exhibited high selectivity by not recognizing the biological target in samples from patients not infected with SARS-CoV-2. The proposed sensor obtained a linear response range estimated from 800 to 4000 copies µL -1 with a regression coefficient of 0.99, and a detection limit of 258.01 copies µL -1 . Therefore, the electrochemical biosensor based on flexible electrode technology represents a promising trend for sensitive molecular analysis of etiologic agent with fast and simple operationalization. In addition to early genetic diagnosis, the biomolecular assay may help to monitor the progression of COVID-19 infection in a novel manner.

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“…Recently, a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) has been developed based on reverse transcription (RT), isothermal amplification, and CRISPR-Cas12 reaction [ 194 ]. The schematic procedure of CRISPR detection systems is shown in Figure 1 D.…”

Section: Non-conventional Methodsmentioning

confidence: 99%

Nucleic Acid Testing of SARS-CoV-2

Yoo

Kim

2021

IJMS

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The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. The polymerase chain reaction (PCR)-based method including reverse transcription-polymerase chain reaction (RT-PCR) is the most widely used assay for the detection of SARS-CoV-2 RNA. Along with the RT-PCR method, digital PCR has emerged as a powerful tool to quantify nucleic acid of the virus with high accuracy and sensitivity. Non-PCR based techniques such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription recombinase polymerase amplification (RT-RPA) are considered to be rapid and simple nucleic acid detection methods and were reviewed in this paper. Non-conventional molecular diagnostic methods including next-generation sequencing (NGS), CRISPR-based assays and nanotechnology are improving the accuracy and sensitivity of COVID-19 diagnosis. In this review, we also focus on standardization of SARS-CoV-2 nucleic acid testing and the activity of the National Metrology Institutes (NMIs) and highlight resources such as reference materials (RM) that provide the values of specified properties. Finally, we summarize the useful resources for convenient COVID-19 molecular diagnostics.

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COVID-19: A challenge for electrochemical biosensors

Cited by 95 publications

References 93 publications

A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor

A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor

Nanostructured sensor platform based on organic polymer conjugated to metallic nanoparticle for the impedimetric detection of SARS-CoV-2 at various stages of viral infection

Nucleic Acid Testing of SARS-CoV-2

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