“…The application of this technology requires high costs, so it is rather difficult to apply, especially in an artificial insemination center in a regional area. Various methods have been used in sperm sexing, such as sedimentation (albumin column), electrophoresis, filtration (sephadex column), and Percoll density gradient centrifugation [2]. Among these methods, the albumin column method is a simple method where the differences in X and Y spermatozoa are measured based on differences in mass and size of spermatozoa, which cause differences in motility.…”