COVID-19 Rapid Antigen Test at Hospital Admission Associated to the Knowledge of Individual Risk Factors Allow Overcoming the Difficulty of Managing Suspected Patients in Hospitals

Filgueiras, Priscilla Soares; Corsini, Camila Amormino; Almeida, Nathalie BF; Assis, Jéssica Vieira de; Pedrosa, Maria Luysa Camargos; Miranda, Daniel  AP de; Gomes, Sarah  VC; Silva, Raphael A; Medeiros, Maria Izabella VARC; Lourenço, Adelina J; Bicalho, Cecília MF; Vilela, Raquel VR; Fernandes, Gabriel R.; Grenfell, Rafaella FQ

doi:10.26502/fjhs.055

Cited by 3 publications

(2 citation statements)

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“…Suspected COVID-19 patients admitted to Hospital da Baleia, Belo Horizonte, Brazil, underwent RT-qPCR testing on nasopharyngeal swabs to detect SARS-CoV-2 [31], following ethical protocols (CEP 42 898 621.9.0000.5091, CONEP 30 428 720.3.0000.5091) with written informed consent. Venous blood samples (5 ml) were collected without anticoagulant, allowed to clot, and serum was extracted by centrifugation (2000 g, 5 min) and stored at −20 • C until analysis.…”

Section: Collection Of Clinical Samplesmentioning

confidence: 99%

Ultrafast and highly sensitive detection of SARS-CoV-2 spike protein by field-effect transistor graphene-based biosensors

Sousa,

Almeida,

Santos

et al. 2024

Nanotechnology

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The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), etiological agent for the coronavirus disease 2019 (COVID-19), has resulted in over 775 million global infections. Early diagnosis remains pivotal for effective epidemiological surveillance despite the availability of vaccines. Antigen-based assays are advantageous for early COVID-19 detection due to their simplicity, cost-effectiveness, and suitability for point-of-care testing (PoCT). This study introduces a graphene field-effect transistor-based biosensor designed for high sensitivity and rapid response to the SARS-CoV-2 spike protein. By functionalizing graphene with monoclonal antibodies and applying short-duration gate voltage pulses, we achieve selective detection of the viral spike protein in human serum within 100 μs and at concentrations as low as 1 fg/mL, equivalent to 8 antigen molecules per μL of blood. Furthermore, the biosensor estimates spike protein concentrations in serum from COVID-19 patients. Our platform demonstrates potential for next-generation PoCT antigen assays, promising fast and sensitive diagnostics for COVID-19 and other infectious diseases.

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Section: Collection Of Clinical Samplesmentioning

confidence: 99%

Ultrafast and highly sensitive detection of SARS-CoV-2 spike protein by field-effect transistor graphene-based biosensors

Sousa,

Almeida,

Santos

et al. 2024

Nanotechnology

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show abstract

“…NAATs, such as reverse transcription PCR, tend to have higher sensitivity and specificity than RATs ( 2 ) but often are more costly and take much longer to process ( 3 , 4 ). Thus, RATs increasingly have been used across the United States for at-home symptom-based testing and asymptomatic screening in healthcare, educational, and public event settings ( 5 ).…”

mentioning

confidence: 99%

Model for Interpreting Discordant SARS-CoV-2 Diagnostic Test Results

Egbelowo,

Fox,

Gibson

et al. 2024

Emerg. Infect. Dis.

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Performance of Antigen Detection Tests for SARS-CoV-2: A Systematic Review and Meta-Analysis

et al. 2022

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Coronavirus disease 2019 (COVID-19) initiated global health care challenges such as the necessity for new diagnostic tests. Diagnosis by real-time PCR remains the gold-standard method, yet economical and technical issues prohibit its use in points of care (POC) or for repetitive tests in populations. A lot of effort has been exerted in developing, using, and validating antigen-based tests (ATs). Since individual studies focus on few methodological aspects of ATs, a comparison of different tests is needed. Herein, we perform a systematic review and meta-analysis of data from articles in PubMed, medRxiv and bioRxiv. The bivariate method for meta-analysis of diagnostic tests pooling sensitivities and specificities was used. Most of the AT types for SARS-CoV-2 were lateral flow immunoassays (LFIA), fluorescence immunoassays (FIA), and chemiluminescence enzyme immunoassays (CLEIA). We identified 235 articles containing data from 220,049 individuals. All ATs using nasopharyngeal samples show better performance than those with throat saliva (72% compared to 40%). Moreover, the rapid methods LFIA and FIA show about 10% lower sensitivity compared to the laboratory-based CLEIA method (72% compared to 82%). In addition, rapid ATs show higher sensitivity in symptomatic patients compared to asymptomatic patients, suggesting that viral load is a crucial parameter for ATs performed in POCs. Finally, all methods perform with very high specificity, reaching around 99%. LFIA tests, though with moderate sensitivity, appear as the most attractive method for use in POCs and for performing seroprevalence studies.

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COVID-19 Rapid Antigen Test at Hospital Admission Associated to the Knowledge of Individual Risk Factors Allow Overcoming the Difficulty of Managing Suspected Patients in Hospitals

Cited by 3 publications

References 0 publications

Ultrafast and highly sensitive detection of SARS-CoV-2 spike protein by field-effect transistor graphene-based biosensors

Ultrafast and highly sensitive detection of SARS-CoV-2 spike protein by field-effect transistor graphene-based biosensors

Model for Interpreting Discordant SARS-CoV-2 Diagnostic Test Results

Performance of Antigen Detection Tests for SARS-CoV-2: A Systematic Review and Meta-Analysis

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