COX2 Effects on endometrial carcinomas progression

Lyndin, Mykola Serhiiovych; Kravtsova, O. I.; Sikora, Kateryna; Yu, Lyndina; Ye, Kuzenko; Awuah, WA; Abdul-Rahman, T; Hyriavenko, Nataliia Ivanivna; Sikora,; Романюк, Анатолий Николаевич

doi:10.1016/j.prp.2022.154082

Cited by 9 publications

(14 citation statements)

References 36 publications

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“…However, these data contradict other studies, which reported lower COX2 expression in EP that did not differ significantly from samples with normal endometrium. Despite the data showing higher COX2 expression in EP than in EH., [17,30,33,[40][41][42][43] we did not find any dependent COX2 expression on EHP subgroups.…”

Section: Discussionmentioning

confidence: 83%

“…[28] COX2 is a crucial enzyme associated with inflammation and tumorigenesis. [11,17,29] It regulates cell proliferation, differentiation, and apoptosis through several autocrine and paracrine transducers. [29,30] COX2 expression may also be associated with inflammatory and dyshormonal disorders of genital organs.…”

Section: Discussionmentioning

confidence: 99%

“…COX2 expression in the luminal and glandular epithelial cells regardless of the menstrual cycle phase. [17] ER and COX2 expression in endometrial tissues was not correlated with age, weight, and BMI (Р ˃ .05; Table 2). However, COX2 expression was inversely correlated with height in EHP samples in general (r = −0.49, P = .037) and in GFEP samples in particular (r = −0.85, P = .034).…”

Section: Ihc Of Er and Cox2 Expressionmentioning

confidence: 99%

“…It is expressed in normal tissues and in many cancers and performs a pleiotropic and multifaceted function in carcinogenesis. [15][16][17] COX2 is involved in regulates resistance to apoptosis, cancer cell proliferation and invasion, angiogenesis, inflammation, and metastasis. Inhibition of the activity of these proteins may contribute to the regression of a malignant tumor, which is a promising therapeutic method in modern oncology.…”

Section: Introductionmentioning

confidence: 99%

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ER and COX2 expression in endometrial hyperplasia processes

Tsyndrenko,

Lyndіn,

Sikora

et al. 2023

Medicine

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Endometrial hyperplastic processes (EHPs) encompass various morphological changes, characterized by an increased ratio of endometrial glands to stroma. These changes manifest as endometrial hyperplasia (EH) and endometrial polyps. The objective of this study was to investigate the expressions of ER and Cyclooxygenase-2 (COX2) in EH and endometrial polyps, and determine their correlation with histological and anthropometric parameters. Tissue samples were obtained during hysteroresectoscopy and divided into 3 groups: non-atypical EH, glandular EP, and glandular-fibrous EP. We examined the immunoprofile of epithelial and stromal cells using rabbit polyclonal anti-COX2 antibodies and rabbit monoclonal anti-ER antibodies (clone SP1). Our results indicate that there is no association between the expressions of ER and COX2 and the type of EHP. Furthermore, the expression levels of ER and COX2 are not influenced by the patients anthropometric parameters. However, tissues with EHPs exhibited significantly higher COX2 expression compared to intact tissues. We also observed a direct correlation between ER and COX2 expression in the endometrial epithelium. The variability in ER and COX2 expressions observed in hyperplastic processes of the endometrium potentially suggests their synergistic involvement in the initiation and progression of EHPs, as well as their potential role in subsequent tumor transformation.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Ihc Of Er and Cox2 Expressionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ER and COX2 expression in endometrial hyperplasia processes

Tsyndrenko,

Lyndіn,

Sikora

et al. 2023

Medicine

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“…Extracellular vesicles (EVs), biological nanoparticles that mediates cell communication and are present in oviductal fluid, play a crucial role in the communication between gamete–oviductal and embryo–oviductal cells [ 40 ]. The organs of the female reproductive system exhibit basal cyclooxygenase 2 (COX2) expression [ 41 ]. At both the mRNA and protein levels, COX2 expression positively correlated with estrogen receptor (ER) expression [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods

Sun

Liu

et al. 2022

IJMS

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The oviduct is a dynamic reproductive organ for mammalian reproduction and is required for gamete storage, maturation, fertilization, and early embryonic development, and it directly affects fecundity. However, the molecular regulation of prolificacy occurring in estrous periods remain poorly understood. This study aims to gain a better understanding of the genes involved in regulating goat fecundity in the proteome and transcriptome levels of the oviducts. Twenty female Yunshang black goats (between 2 and 3 years old, weight 52.22 ± 0.43 kg) were divided into high- and low-fecundity groups in the follicular (FH and FL, five individuals per group) and luteal (LH and LL, five individuals per group) phases, respectively. The DIA-based high-resolution mass spectrometry (MS) method was used to quantify proteins in twenty oviducts. A total of 5409 proteins were quantified, and Weighted gene co-expression network analysis (WGCNA) determined that the tan module was highly associated with the high-fecundity trait in the luteal phase, and identified NUP107, ANXA11, COX2, AKP13, and ITF140 as hub proteins. Subsequently, 98 and 167 differentially abundant proteins (DAPs) were identified in the FH vs. FL and LH vs. LL comparison groups, respectively. Parallel reaction monitoring (PRM) was used to validate the results of the proteomics data, and the hub proteins were analyzed with Western blot (WB). In addition, biological adhesion and transporter activity processes were associated with oviductal function, and several proteins that play roles in oviductal communication with gametes or embryos were identified, including CAMSAP3, ITGAM, SYVN1, EMG1, ND5, RING1, CBS, PES1, ELP3, SEC24C, SPP1, and HSPA8. Correlation analysis of proteomics and transcriptomic revealed that the DAPs and differentially expressed genes (DEGs) are commonly involved in the metabolic processes at the follicular phase; they may prepare the oviductal microenvironment for gamete reception; and the MAP kinase activity, estrogen receptor binding, and angiotensin receptor binding terms were enriched in the luteal phase, which may be actively involved in reproductive processes. By generating the proteome data of the oviduct at two critical phases and integrating transcriptome analysis, we uncovered novel aspects of oviductal gene regulation of fecundity and provided a reference for other mammals.

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