Cph1p negatively regulates MDR1 involved in drug resistance in Candida albicans

Lo, Hsiu‐Jung; Tseng, Kuo-Yun; Kao, Yeong-Yi; Tsao, Ming-Yang; Lo, Han-Lun; Yang, Yun-Liang

doi:10.1016/j.ijantimicag.2015.01.017

Cited by 14 publications

(6 citation statements)

References 28 publications

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“…In the past two decades, infections caused by C. albicans have increased significantly (Lo et al, 2015). Invasive candidiasis has an estimated mortality about 40%, even with the use of antifungal drugs (Horn et al, 2009; Lu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Responses of Candida albicans to Antimicrobial Peptide MAF-1A

et al. 2017

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Candida albicans is a major fungal pathogen in humans. Novel antifungal agents are urgent demanded due to the challenges of the resistance. Antimicrobial peptides (AMPs) are critical components of the innate immune system against pathogenic microorganism infection. MAF-1A is a novel cationic AMP that comes from Musca domestica and is effective against C. albicans, but the antifungal mechanism remains unclear. In this study, we performed a transcriptomics analysis in C. albicans using RNA-seq technique under the treatment of MAF-1A. A total of 5654 genes were identified. Among these, 1032 were differentially expressed genes (DEGs), including 575 up-regulated genes and 457 down-regulated genes. In these DEGs, genes encoding ergosterol metabolism and fatty acid biosynthesis were identified to be significantly down-regulated, while genes associated with oxidative stress response and cell wall were identified to be significantly up-regulated. Using pathway enrichment analysis, 12 significant metabolic pathways were identified, and ribosome, oxidative phosphorylation, citrate cycle were mainly involved. The results revealed that MAF-1A induces complex responses in C. albicans. This study provides evidence that MAF-1A may inhibit the growth through affect multi-targets in C. albicans cells.

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Section: Introductionmentioning

confidence: 99%

Transcriptional Responses of Candida albicans to Antimicrobial Peptide MAF-1A

et al. 2017

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“…C. albicans is an opportunistic pathogen of humans and an endogenous member of the human microbiota. In the past two decades, infections caused by C. albicans have increased significantly 3 . A characteristic feature of C. albicans is its ability to grow either as unicellular budding yeast or in filamentous form 1 .…”

mentioning

confidence: 99%

Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans

Shi

Dai

et al. 2016

Sci Rep

101

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The antifungal activity, kinetics, and molecular mechanism of action of garlic oil against Candida albicans were investigated in this study using multiple methods. Using the poisoned food technique, we determined that the minimum inhibitory concentration of garlic oil was 0.35 μg/mL. Observation by transmission electron microscopy indicated that garlic oil could penetrate the cellular membrane of C. albicans as well as the membranes of organelles such as the mitochondria, resulting in organelle destruction and ultimately cell death. RNA sequencing analysis showed that garlic oil induced differential expression of critical genes including those involved in oxidation-reduction processes, pathogenesis, and cellular response to drugs and starvation. Moreover, the differentially expressed genes were mainly clustered in 19 KEGG pathways, representing vital cellular processes such as oxidative phosphorylation, the spliceosome, the cell cycle, and protein processing in the endoplasmic reticulum. In addition, four upregulated proteins selected after two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE) analysis were identified with high probability by mass spectrometry as putative cytoplasmic adenylate kinase, pyruvate decarboxylase, hexokinase, and heat shock proteins. This is suggestive of a C. albicans stress responses to garlic oil treatment. On the other hand, a large number of proteins were downregulated, leading to significant disruption of the normal metabolism and physical functions of C. albicans.

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“…Because translocation and dissemination by C. albicans has also been shown to be dependent on the ability to transform to the hyphal form ( Koh et al., 2008 ), we next tested C . albicans mutants with deletions in genes involved in formation of pseudohyphae and hyphae (the transcription factors CPH1 and EFG1) ( Lo et al, 1997 , 2015 ). Compared with the isogenic wild type, infection with cph1- , efg1- , and cph1/efg1 -deficient C. albicans mutants resulted in significantly decreased mortality ( Figure 3H ), suggesting that fungal virulence factors associated with adhesion and hyphal filamentation promote invasion in our mucosal injury model.…”

Section: Resultsmentioning

confidence: 99%

“…Although we observed no change in the ability of CotH RNAi spores to adhere to extracellular matrix (ECM) compounds in vitro (Figure S4C), zebrafish larvae express a homolog of the well-described epithelial CotH target GRP78 (Gebremariam et al, 2014;Liu et al, 2010;Thakur et al, 2014). Because translocation and dissemination by C. albicans has also been shown to be dependent on the ability to transform to the hyphal form (Koh et al, 2008), we next tested C. albicans mutants with deletions in genes involved in formation of pseudohyphae and hyphae (the transcription factors CPH1 and EFG1) (Lo et al, 1997(Lo et al, , 2015. Compared with the isogenic wild type, infection with cph1-, efg1-, and cph1/efg1-deficient C. albicans mutants resulted in significantly decreased mortality (Figure 3H), suggesting that fungal virulence factors associated with adhesion and hyphal filamentation promote invasion in our mucosal injury model.…”

Section: Spatial and Temporal Events Driving Invasive Fungal Infection After Epithelial Cell Extrusionmentioning

confidence: 99%

EGF-mediated suppression of cell extrusion during mucosal damage attenuates opportunistic fungal invasion

et al. 2021

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EGF-mediated suppression of cell extrusion during mucosal damage attenuates opportunistic fungal invasionGraphical abstract Highlights d Inducible epithelial cell loss allows modeling of fungal infection in zebrafish larvae d Extrusion of numerous cells exposes laminin and increases fungal adhesion and invasion d Cell extrusion stimulates epigen, mmp13a, and il1b expression and neutrophil recruitment d rhEPGN suppresses extrusion to provide protective effects against fungal invasion

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Cph1p negatively regulates MDR1 involved in drug resistance in Candida albicans

Cited by 14 publications

References 28 publications

Transcriptional Responses of Candida albicans to Antimicrobial Peptide MAF-1A

Transcriptional Responses of Candida albicans to Antimicrobial Peptide MAF-1A

Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans

EGF-mediated suppression of cell extrusion during mucosal damage attenuates opportunistic fungal invasion

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