CPTF1, a CREB-Like Transcription Factor, Is Involved in the Oxidative Stress Response in the Phytopathogen <i>Claviceps purpurea</i> and Modulates ROS Level in Its Host <i>Secale cereale</i>

Nathues, Eva; Joshi, Suchitra; Tenberge, Klaus B.; Driesch, Marcell von den; Oeser, Birgitt; Bäumer, Nicole; Mihlan, Martina; Tudzynski, Paul

doi:10.1094/mpmi.2004.17.4.383

Cited by 81 publications

(76 citation statements)

References 56 publications

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“…Thus, the Yap1-controlled ROS detoxification system serves an important function during the early U. maydis infection phase. In the Claviceps purpurea/rye interaction it has been demonstrated that the deletion of a CREBlike transcription factor, CPTF1, which positively controls catalase production, also induces an oxidative burst (Nathues et al, 2004) similar to what we have observed in yap1 mutants of U. maydis. However, in this case, it is speculated that CPTF1 negatively controls the activity of a fungal NADPH oxidase (i.e., when CPTF1 is deleted, the fungus would produce elevated levels of H 2 O 2 , which then in turn would trigger the oxidative burst) (Nathues et al, 2004).…”

Section: Discussionsupporting

confidence: 84%

AnUstilago maydisGene Involved in H2O2 Detoxification Is Required for Virulence

2007

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The fungus Ustilago maydis is a biotrophic pathogen of maize (Zea mays). In its genome we have identified an ortholog of YAP1 (for Yeast AP-1-like) from Saccharomyces cerevisae that regulates the oxidative stress response in this organism. yap1 mutants of U. maydis displayed higher sensitivity to H 2 O 2 than wild-type cells, and their virulence was significantly reduced. U. maydis yap1 could partially complement the H 2 O 2 sensitivity of a yap1 deletion mutant of S. cerevisiae, and a Yap1-green fluorescent protein fusion protein showed nuclear localization after H 2 O 2 treatment, suggesting that Yap1 in U. maydis functions as a redox sensor. Mutations in two Cys residues prevented accumulation in the nucleus, and the respective mutant strains showed the same virulence phenotype as Dyap1 mutants. Diamino benzidine staining revealed an accumulation of H 2 O 2 around yap1 mutant hyphae, which was absent in the wild type. Inhibition of the plant NADPH oxidase prevented this accumulation and restored virulence. During the infection, Yap1 showed nuclear localization after penetration up to 2 to 3 d after infection. Through array analysis, a large set of Yap1-regulated genes were identified and these included two peroxidase genes. Deletion mutants of these genes were attenuated in virulence. These results suggest that U. maydis is using its Yap1-controlled H 2 O 2 detoxification system for coping with early plant defense responses.

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Section: Discussionsupporting

confidence: 84%

AnUstilago maydisGene Involved in H2O2 Detoxification Is Required for Virulence

2007

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“…Other transcripts with potential roles in metabolism of reactive oxygen species or redox homeostasis included a putative Cu/Zn superoxide dismutase, a catalase (1-L10-SP6/T7) and a homologue of the cAMP response element (CREB-like) transcription factor CPTF1, which regulates catalase activity and contributes to virulence in the biotrophic plant pathogen Claviceps purpurea (Nathues et al, 2004).…”

Section: Putative Virulence and Pathogenicity Genesmentioning

confidence: 99%

Identification of soluble secreted proteins from appressoria of Colletotrichum higginsianum by analysis of expressed sequence tags

et al. 2008

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The hemibiotrophic ascomycete Colletotrichum higginsianum causes anthracnose disease on brassica crops and the model plant Arabidopsis. Melanized appressoria pierce the host cuticle and cell wall to form specialized biotrophic hyphae inside living epidermal cells. To identify proteins secreted by appressoria that may function as virulence effectors, a cDNA library was prepared from mature appressoria formed in vitro. Bidirectional sequencing of 980 clones generated 1442 high-quality expressed sequence tags (ESTs), comprising 518 unique sequences. BLASTX analysis showed that 353 (68 %) of these had significant similarity to entries in the NCBI non-redundant protein database, of which 49 were also homologous to experimentally verified fungal pathogenicity genes. ORFs were predicted ab initio from the unique sequences and screened for potential signal peptides using SignalP. Fifty-three unique sequences (10 %) were predicted to encode proteins entering the secretory pathway, of which 26 were likely to be soluble secreted proteins. For a selected subset of these, RT-PCR showed that seven genes that encode secreted proteins of unknown function, including two Colletotrichum-specific genes, are upregulated in appressoria and expressed early during plant infection, and therefore represent candidate effectors. INTRODUCTIONThe ascomycete genus Colletotrichum comprises one of the most economically damaging groups of plant-pathogenic fungi, causing anthracnose diseases and blights on an enormous range of dicot and monocot crop plants in temperate, tropical and subtropical regions (Bailey & Jeger, 1992). The crucifer anthracnose pathogen Colletotrichum higginsianum has a wide host range, attacking many cultivated forms of Brassica and Raphanus as well as wild crucifers, including the model plant Arabidopsis thaliana (Narusaka et al., 2004;O'Connell et al., 2004). C. higginsianum employs a two-stage, hemibiotrophic infection strategy: after melanized appressoria breach the host cuticle and cell wall, the fungus initially grows biotrophically inside living epidermal cells, before entering a destructive necrotrophic phase in which host tissues are killed and extensively macerated by cell-wall-degrading enzymes. During the biotrophic phase, C. higginsianum differentiates specialized intracellular primary hyphae similar to haustoria, which expand and locally modify the host plasma membrane (Shimada et al., 2006). However, in contrast to obligately biotrophic pathogens of Arabidopsis, Colletotrichum can be cultured axenically and stably transformed using a variety of methods; it is also a haploid organism with uninucleate conidia, which facilitates mutational analyses. This pathosystem therefore provides an attractive model for studying biotrophy, in which both partners in the interaction are genetically tractable.Whole-genome sequencing projects have revealed that fungal and oomycete plant pathogens possess large repertoires of secreted proteins, which can play diverse roles in pathogenicity and interactions with host cells (Dean...

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“…By comparing with superoxide (O 2 •), hydroxyl radical (•OH) and other ROS, H 2 O 2 is the most stable compound (Cheeseman 2007). Within the cellular concentration, H 2 O 2 behaves as a signaling molecule and it is used for the induction of gene expression, helpful for physiological process (Nathues et al 2004). H 2 O 2 becomes more dangerous when it reacts with some transition metals as Fe 2+ and produces two molecules of OH -by Fenton reaction (Becana et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Interaction of polyamine on oxidative stress induced by exogenously applied hydrogen peroxide in Salvinia natans Linn

Mandal¹,

Ghosh²,

Adak³

et al. 2013

Theor. Exp. Plant Physiol.

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A hydroponics experiment was carried out to study the role of hydrogen peroxide (H 2 O 2 ) through changes of oxidative stress and antioxidation activity in Salvinia natans Linn. H 2 O 2 is an active oxygen species, widely generated in many biological systems, and mediates various physiological and biochemical processes in plants. In this study, we demonstrated that exogenous H 2 O 2 causes the degradation of pigments and induces the oxidative stress and the antioxidation activity. These effects enhanced with increments of applied H 2 O 2 concentration. Application of polyamine reduced the generation and the accumulation of H 2 O 2 and modulated the enzyme activities.

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CPTF1, a CREB-Like Transcription Factor, Is Involved in the Oxidative Stress Response in the Phytopathogen Claviceps purpurea and Modulates ROS Level in Its Host Secale cereale

Cited by 81 publications

References 56 publications

AnUstilago maydisGene Involved in H2O2 Detoxification Is Required for Virulence

AnUstilago maydisGene Involved in H2O2 Detoxification Is Required for Virulence

Identification of soluble secreted proteins from appressoria of Colletotrichum higginsianum by analysis of expressed sequence tags

Interaction of polyamine on oxidative stress induced by exogenously applied hydrogen peroxide in Salvinia natans Linn

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