CQ Researcher

Downregulation of Betaig-h3 gene is causally linked to tumorigenic phenotype in asbestos treated immortalized human bronchial epithelial cells

“…Although BEP2D cells are immortalized by HPV-18 that disturbs both normal p53 and RB functions, they are non-tumorigenic even in late passage (Hei et al, 1997Willey et al, 1991). This suggests that additional genetic alterations are needed in the tumorigenic conversion of these cells by asbestos.…”

“…The human papillomavirus-immortalized human bronchial epithelial (BEP2D) cells (Willey et al, 1991) and their asbestos induced tumorigenic variants (Hei et al, 1997;Piao et al, 2001) were maintained in serum-free LHC-8 medium supplemented with various growth factors (Biofluid, Rockville, MD, USA). Primary human bronchial epithelial (NHBE) cells and human mammary epithelial cells (HMEC) were purchased from Clonetics BioWhittaker (Walkersville, MD, USA) and were maintained in serum-free BEGM and MEBM medium supplemented growth factors.…”

Human Papillomavirus Infection and Lung Cancer

“…These oncogene proteins can regulate expression of several target genes and proteins, which derives in promoted lung cell proliferation, angiogenesis, and cell immortalization. Among the genes and proteins affected are p53, pRb, HIF-1α, VEGF, IL-6, IL-10, Mcl-1, Bcl-2, cIAP-2, EGFR, FHIT, hTERT, HER-2, ALK, ROS1, and AhR [55,[87][88][89][90]. Evidence which supports the association between HPV infection and lung cancer continues to grow, but debate will likely continue due to heterogeneous methodologies for HPV detection in lung tissue.…”