The purpose of this research was to estimate the Zn 2+ biosorption by biomass of Humicola phialophoroides fungus isolated from creek sediments in a zinc mine area in Thailand. The Langmuir isotherm model gave a better fit for the result data more than Freundlich, Temkin and Dubinin-Radushkevich isotherm models. Zn 2+ removal ability in all H. phialophoroides biomass types as increasing Zn 2+ concentration had the same pattern, but biomass pretreated by NaOH showed better Zn 2+ removal ability than viable biomass, nonviable biomass, and HNO 3 pretreated biomass. Maximum Zn 2+ biosorption of biomass pretreated with NaOH took place at initial solution at pH 8 after 150 min. In addition, Zn 2+ was well adsorped by NaOH pretreated biomass at temperature higher than 70 °C, while desorption experiments indicated that the desorption efficiency with 0.1 M HNO 3 solution reached 92.31%.