Cr(VI) reduction by Pseudomonas aeruginosa immobilized in a polyvinyl alcohol/sodium alginate matrix containing multi-walled carbon nanotubes

Pang, Ya; Zeng, Guangming; Tang, Lin; Zhang, Yi; Liu, Yuanyuan; Lei, Xiaoxia; Wu, Mengshi; Li, Zhen; Liu, Can

doi:10.1016/j.biortech.2011.08.078

Cited by 82 publications

(28 citation statements)

References 13 publications

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“…Free cells were able the reduce 100 mg/L of Cr(VI) with the same efficiency for two cycles in the non-modified medium, whereas this efficiency was maintained until the third cycle using washed cells in a fresh LB medium after Cr(VI) reduction ability became lower. Similarly, Farag and Zaki (2010) have reported that Na-alginate immobilized Pseudomonas strain could be reused three times without losing their chromium reduction activity for 3 days, while Pang et al (2011) have reported that the Cr(VI) reduction efficiency of immobilized Pseudomonas aeruginosa cells declined significantly at the seventh time. They explained this by the loss of P. aeruginosa of reductase in the long-term shaking incubation and repeated wash, as well as the deterioration or mutation of P. aeruginosa (Farag and Zaki 2010).…”

Section: Cr(vi) Reduction Tests Using Repeated Addition Of Cr(vi) Alimentioning

confidence: 98%

Leucobacter chromiireducens CRB2, a new strain with high Cr(VI) reduction potential isolated from tannery-contaminated soil (Fez, Morocco)

et al. 2015

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A new chromate-reducing bacterial strain was isolated from soil contaminated with tannery waste. Based on 16S rRNA gene sequence analyses, this strain was identified as Leucobacter chromiireducens CRB2. This bacterium had high multiresistance against heavy metals with a MIC of 700 mg/L Cr(VI) and was able to reduce Cr(VI) both aerobically and anaerobically. The optimum pH and temperature for Cr(VI) reduction were pH 8.0 and 30°C, respectively. Glycerol (10 mM) was the most efficient carbon source for Cr(VI) reduction by the strain followed by glucose. Moreover, Cr(VI) reduction by L. chromiireducens CRB2 was unaltered in the presence of other oxyanions. Bacterial cells immobilized in Na-alginate beads showed a high Cr(VI) reduction rates and exhibited an ability to repeatedly reduce Cr(VI). Therefore, immobilized cells were more effective than free cells in Cr(VI) reduction. Resting and permeabilized cell assays provided the better evidence of the presence of an enzymatic chromate reduction in L. chromiireducens CRB2. Assays conducted with cytosolic and particulate fractions of L. chromiireducens confirmed the role of cytosolic proteins in Cr(VI) reduction. Cr(VI) reduction by L. chromiireducens was mediated by a soluble enzyme contained in the cytoplasm after its adsorption on the cell surface. To the best of our knowledge, this is the first report studying parameters affecting Cr(VI) reduction and describing Cr(VI) removal mechanism by strain of L. chromiireducens.

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Section: Cr(vi) Reduction Tests Using Repeated Addition Of Cr(vi) Alimentioning

confidence: 98%

Leucobacter chromiireducens CRB2, a new strain with high Cr(VI) reduction potential isolated from tannery-contaminated soil (Fez, Morocco)

et al. 2015

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“…The average phenol degradation rate was calculated using the formula: C 0 − C t /t, [14] where C 0 is the initial phenol concentration (mM or mg L −1 ), C t is the phenol concentration (mM or mg L −1 ) at time t, and t is the incubation time (h).…”

Section: Growth and Phenol Degradation Studiesmentioning

confidence: 99%

Assessment of phenol-degrading ability ofAcinetobactersp. B9 for its application in bioremediation of phenol-contaminated industrial effluents

Bhattacharya

Gupta

Kaur

et al. 2015

Chemistry and Ecology

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Successful bioremediation of a phenol-contaminated environment requires application of those microbial strains that have acquired phenol tolerance and phenol-degrading abilities. A newly isolated strain B9 of Acinetobacter sp. was adapted to a high phenol concentration by growing sequentially from low-to highstrength phenol. The acclimatised strain was able to grow and completely degrade up to 14 mM of phenol in 136 h. The degradation rates were found to increase with an increase in the phenol concentration from 2.0 to 7.5 mM. The strain preferred neutral to alkaline pH range for growth and phenol degradation, with the optimum being pH 8.0. The optimum temperature for phenol degradation was found to be in the range of 30-35°C. Transmission electron micrographs showed a disorganised and convoluted cell membrane in the case of phenol-stressed cells, showing a major effect of phenol on the membrane. Enzymatic and gas chromatography-mass spectrometry studies show the presence of an ortho-cleavage pathway for phenol degradation. Efficient phenol degradation was observed even in the presence of pyridine and heavy metals as co-toxicants showing the potential of strain in bioremediation of industrial wastes. Application of strain B9 to real tannery wastewater showed 100% removal of initial 0.5 mM phenol within 48 h of treatment.

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“…2) could be attributed to the loss of matrix stability as well as release of B. sphaericus cells and reductase in the long term shaking incubation and repeated wash as observed in Pseudomonas aeruginosa (Pang et al 2011), Desulfovibrio vulgaris and Microbacterium sp. (Humphries et al 2005) The deterioration or changes of biochemical features of the strain during the process of reduction could be another possibility (Pang et al 2011). The understanding of this phenomenon could be used to improve the stability of B. sphaericus cells in order to apply it in an efficient continuous reduction system.…”

Section: Reuse Of Beads Under Optimized Conditionmentioning

confidence: 99%

Hexavalent chromium reduction by immobilized cells of Bacillus sphaericus AND 303

Pal

Datta

Paul

2013

Braz. arch. biol. technol.

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Cr(VI) reduction by Pseudomonas aeruginosa immobilized in a polyvinyl alcohol/sodium alginate matrix containing multi-walled carbon nanotubes

Cited by 82 publications

References 13 publications

Leucobacter chromiireducens CRB2, a new strain with high Cr(VI) reduction potential isolated from tannery-contaminated soil (Fez, Morocco)

Leucobacter chromiireducens CRB2, a new strain with high Cr(VI) reduction potential isolated from tannery-contaminated soil (Fez, Morocco)

Assessment of phenol-degrading ability ofAcinetobactersp. B9 for its application in bioremediation of phenol-contaminated industrial effluents

Hexavalent chromium reduction by immobilized cells of Bacillus sphaericus AND 303

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