CR1 exon variants are associated with lowered CR1 expression and increased susceptibility to SLE in a<i>Plasmodium falciparum</i>endemic population

Panda, Aditya K.; Ravindran, Balachandran; Das, B. K.

doi:10.1136/lupus-2016-000145

Cited by 13 publications

(11 citation statements)

References 39 publications

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“…However, in an earlier observation, we have demonstrated protection against severe malaria and malarial death in complement receptor 1 variants and concluded a possible reason for higher prevalence of CR1 mutants in malaria endemic areas 14 . We had also observed that CR-1 mutants are susceptible to development of SLE and lupus nephritis since they expressed lower surface CR1 which affects clearance of apoptotic debris 73 . Furthermore, similar association of FcγRIIb variant (codon 232) with susceptibility to SLE and protection against P .…”

Section: Discussionmentioning

confidence: 94%

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TNF-α promoter polymorphisms (G-238A and G-308A) are associated with susceptibility to Systemic Lupus Erythematosus (SLE) and P. falciparum malaria: a study in malaria endemic area

Mahto

Tripathy

Meher

et al. 2019

Sci Rep

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Tumor necrosis factor-α (TNF-α) is a proinflammatory cytokine associated with autoimmune and infectious diseases. Importance of TNF-α in P . falciparum malaria and systemic lupus erythematosus (SLE) have been demonstrated. However, association of functional promoter variants with SLE and malaria is lacking in malaria endemic population. A total of 204 female SLE patients and 224 age and sex matched healthy controls were enrolled in the study. Three hundred fourteen P . falciparum infected patients with different clinical phenotypes were included. TNF-α polymorphisms (G-238A & G-308A) were genotyped by PCR-RFLP. Plasma levels of TNF-α was quantified by ELISA. Heterozygous mutants and minor alleles of TNF-α (G-238A and G-308A) polymorphisms were significantly higher in SLE patients compared to healthy controls and associated with development of lupus nephritis. In addition, both promoter variants were associated with severe P . falciparum malaria. SLE patients demonstrated higher levels of plasma TNF-α compared to healthy controls. TNF-α (G-238A and G-308A) variants were associated with higher plasma TNF-α. In conclusion, TNF-α (G-238A & G-308A) variants are associated with higher plasma TNF-α levels in SLE patients residing in malaria endemic areas and could be a contributing factor in the development of SLE and susceptibility to severe P . falciparum malaria.

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Section: Discussionmentioning

confidence: 94%

“…Gender wise analysis has been recommended in numerous earlier reports of genetic association studies 73,75,76 . SLE is a chronic inflammatory autoimmune disorder and mostly prevalent in females 77 .…”

Section: Methodsmentioning

confidence: 99%

TNF-α promoter polymorphisms (G-238A and G-308A) are associated with susceptibility to Systemic Lupus Erythematosus (SLE) and P. falciparum malaria: a study in malaria endemic area

Mahto

Tripathy

Meher

et al. 2019

Sci Rep

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“…In addition to being an antigen with a lower frequency in Europe, it has been reported in several publications that CR1 p.His1208Arg is correlated with low CR1 cell surface expression. 11,12 We therefore speculated that our failure to detect anti-CR1 p.1208Arg in the inhibition assay was because we needed homozygous CR1 p.1208 (Arg/Arg) RBCs for a reaction in the IAT.…”

Section: Anti-cr1 P1208argmentioning

confidence: 99%

Two novel antithetical KN blood group antigens may contribute to more than a quarter of all KN antisera in Europe

Grueger

Zeretzke²,

Habicht³

et al. 2020

Transfusion

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Background All antigens described in the KN blood group system are located in the long homologous repeat D (LHR‐D) of complement receptor 1 (CR1). While there have been reports that some sera react only with the long homologous repeat C (LHR‐C), the antigens in LHR‐C are unknown. Study Design and Methods Recombinant LHR‐C and LHR‐D were used to identify antibodies directed against LHR‐C of CR1, into which a point mutation was introduced to characterize the underlying blood group antigens. In addition, database studies to define haplotypes of CR1 were performed. Results Several antisera were identified that were specific against CR1 p.1208His and against CR1 p.1208Arg, located in LHR‐C. Fifteen KN haplotypes were found in the Ensembl genome browser. It was shown that due to a linkage disequilibrium anti‐CR1 p.1208His may be mistaken for anti‐KCAM. Conclusion A novel antithetical KN blood group antigen pair was found at position p.1208 of CR1, for which the names DACY and YCAD are proposed. Antibodies against these two novel antigens seem to contribute to more than a quarter of all KN sera in Europe.

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“…Studies in humans revealed erythrocyte complement receptor 1 (E-CR1) density polymorphisms, which vary by ethnic group (Panda, Ravindran & Das, 2016). Three polymorphic phenotypes, related to the expression levels of CR1 on erythrocytes, exist in Caucasian (Opi et al, 2016) subjects.…”

Section: Introductionmentioning

confidence: 99%

Differences in expression density and molecular weight of CR1-Like in erythrocytes of landrace swine

Zhang

Chun

Jia

et al. 2018

Preprint

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Background. Porcine erythrocytes express complement receptor 1-like (CR1-like), which is involved in immune adherence. Methods. In this study, porcine erythrocyte samples were collected from fifty-five individual Landrace swine to characterize differences in porcine CR1-like. Flow cytometry analysis was performed to examine the porcine differences in CR1-like expression density and immunoprecipitation, SDS-PAGE and Western blot were performed to detect variations in porcine CR1-like molecular weights. Results. Different mean fluorescence intensities (MFI) of porcine erythrocytes were identified in three groups as 33.016±2.889 (40.0%), 59.974±9.299 (45.5%) and 131.241±8.375 (14.5%). Under reduced condition, three porcine CR1-like molecular weight variants were identified as 85.280±0.935 kDa (9.09%), 123.939±2.752 kDa (14.55%) and 136.696±2.028 kDa (76.36%). Discussion. CR1-like was dispersed on the surface of porcine erythrocytes and promoted immune adherence. There have been no reports on whether differences in the expression levels and/or molecular weights of CR1-like in erythrocytes represent diversity in different individuals, and if so, whether this diversity influences the immune adherence of erythrocytes and/or whether the diversity is associated with CR1-like polymorphisms. At present, five candidate genes that are related to the differences above were found. Research examining erythrocyte immune adherence and CR1-like genes is under way. These results will provide theoretical data for future studies of the immunological mechanism of CR1-like in porcine erythrocytes.

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CR1 exon variants are associated with lowered CR1 expression and increased susceptibility to SLE in aPlasmodium falciparumendemic population

Cited by 13 publications

References 39 publications

TNF-α promoter polymorphisms (G-238A and G-308A) are associated with susceptibility to Systemic Lupus Erythematosus (SLE) and P. falciparum malaria: a study in malaria endemic area

TNF-α promoter polymorphisms (G-238A and G-308A) are associated with susceptibility to Systemic Lupus Erythematosus (SLE) and P. falciparum malaria: a study in malaria endemic area

Two novel antithetical KN blood group antigens may contribute to more than a quarter of all KN antisera in Europe

Differences in expression density and molecular weight of CR1-Like in erythrocytes of landrace swine

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