Crab collagenase in wound debridement

Glyantsev, S. P.; Vishnevsky, A. V.; Adamyan, A. A.; Sakharov, Ivan Yu.

doi:10.12968/jowc.1997.6.1.13

Cited by 23 publications

(15 citation statements)

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“…We identified 11 studies that compared collagenase with a petrolatum-based or other ointment, autolytic debridement via a hydrogel or polyacrylate dressing, surgical excision, silver sulfadiazine, and alternative enzymatic agents (papain-urea and trypsin/chymotrypsin) [15][16][17][18][19][20][21][22][23][24][25] (Table 1). Six randomized clinical trials were identified that compared collagenase derived from a bacterial source to a placebo, comprising a heat-inactivated collagenase ointment or a petrolatum-based ointment never charged with the enzymatic agent.…”

Section: ■ Methodsmentioning

confidence: 99%

“…Boxer and coworkers 15 Lee and Ambrus 16 Varma and coworkers 17 Palmieri and Magri 18 Fu and coworkers 19,c Milne 20 Soroff and Sasvary 21 Konig and coworkers 22 Alvarez and coworkers 23 Glyantsev and coworkers 24 Ozcan and coworkers 25 Pullen et al 25 Collagenase provided more rapid removal of necrotic tissue from the wound bed than placebo…”

Section: Debriding Agent Outcomesmentioning

confidence: 99%

“…Glyantsev and associates 24 reported in vitro, in vivo, and clinical studies, comparing a collagenase-based ointment to a trypsin/chymotrypsin debriding agent commercially available in Russia. This review is limited to the results reported from the patient-based trial.…”

Section: Collagenase Ointment Versus Other Active Treatmentsmentioning

confidence: 99%

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Collagenase for Enzymatic Debridement

Ramundo

Gray

2009

Journal of Wound, Ostomy &Amp; Continence Nursing

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Debridement is an essential component of wound bed preparation. Various techniques of debridement are commonly used in clinical practice. This systematic review summarizes and ranks evidence concerning the safety and efficacy of the selective enzymatic debriding agent collagenase. Studies were identified comparing collagenase to inactive (sham) ointment and to alternate techniques for debridement including autolytic and surgical. A preponderance of evidence from this systematic review confirms that collagenase ointment is a safe and effective choice for debridement of cutaneous ulcers and burn wounds.

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Section: ■ Methodsmentioning

confidence: 99%

Section: Debriding Agent Outcomesmentioning

confidence: 99%

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Collagenase for Enzymatic Debridement

Ramundo

Gray

2009

Journal of Wound, Ostomy &Amp; Continence Nursing

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“…Preparations of collagenolytic enzymes from the Antarctic krill and King Crab species have been successfully evaluated in wound debridement [7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Stability and effects of organic solvents on endopeptidases from the gastric fluid of the marine crab Cancer pagurus

Saborowski

Sahling

Toro³

et al. 2004

Journal of Molecular Catalysis B: Enzymatic

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Activities of proteolytic enzymes, represented by azocasein digestion (total protease), trypsin, and chymotrypsin from the gastric fluid of the marine crab, Cancer pagurus, were evaluated for operational parameters like pH-optimum, thermal stability, and effectors such as metal ions and organic solvents. Total protease activity was 98.5 ± 4.3 E 366 min −1 ml −1 . Trypsin and chymotrypsin activities amounted to 14.7 ± 1.5 U ml −1 and 197 ± 59 U ml −1 , respectively. The maximum activity of total protease appeared at pH 5-7, that of trypsin at pH 7-9, and that of chymotrypsin at pH 6.5-7. Trypsin and chymotrypsin activities were slightly activated by Ca 2+ , but were drastically reduced by Zn 2+ . In the presence of 2-propanol, trypsin activity was enhanced 8-fold at 30• C. Highest amplification of activity of up to 30-fold, however, appeared below 12• C. The effects of ethanol and acetone were less distinct. These caused a 2.5-fold increase of activity. Methanol was the least activating solvent.Activities of all enzymes remained stable for up to 127 days at 5• C, keeping up to 70% of the initial activity. When stored at 25• C for 120 days, total protease decreased to about 40% and trypsin and chymotrypsin activities kept more than 60% of initial activity. Zymograms showed five major and several minor proteolytic bands with apparent molecular weight of 20-45 kDa. After long-term storage at room temperature, the major bands remained active, while some bands with minor activity disappeared. This proteolytic enzyme preparation from the gastric fluid of crab seems suitable for biotechnological application at neutral pH and in the presence of organic solvents.

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“…Mit aus der Leber und Pankreas von Paralithodes camtschatica (Königskrabbe) isolierter Kollagenase konnte sowohl in vitro als auch in vivo bei Patienten mit Ulcera crurum ein effektives Débridement gezeigt werden [19,43]. Ein kommerziell erhältliches Präparat,das diese Kollagenasen beinhaltet, wurde bislang nicht hergestellt.…”

Section: Effekte Der Kollagenase Auf Die Wundheilungunclassified

Konditionierung chronischer Wunden mittels proteolytischer Enzyme

Dissemond

Goos

2003

Der Hautarzt

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In the past few years, several different proteolytic enzymes have been shown to be effective in conditioning the chronic wound beds. The use of proteolytic enzymes in wound-bed preparation enables a selective painless debridement. Moreover multiple positive effects on formation of granulation tissue and re-epithelialization have been observed. Therefore, a faster overall healing of chronic wounds may result. Moreover, numerous positive effects on a rapid induction of granulation are discussed. The supporting data do not fulfill the criteria of evidence-based medicine. For most chronic wounds today, enzymatic debridement represents a first choice method especially for outpatients and perhaps in combination with surgical debridement.

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Crab collagenase in wound debridement

Cited by 23 publications

References 0 publications

Collagenase for Enzymatic Debridement

Collagenase for Enzymatic Debridement

Stability and effects of organic solvents on endopeptidases from the gastric fluid of the marine crab Cancer pagurus

Konditionierung chronischer Wunden mittels proteolytischer Enzyme

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