CRABP1 and CRABP2 Protein Levels Correlate with Each Other but Do Not Correlate with Sensitivity of Breast Cancer Cells to Retinoic Acid

Еникеев, А. Д.; Komelkov, A. V.; Axelrod, Maria E; Galetsky, Sergey A.; Кузьмичев, С. А.; Tchevkina, Elena M.

doi:10.1134/s0006297921020103

Cited by 9 publications

(4 citation statements)

References 44 publications

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“…The CRABP1 gene produces the Cellular retinoic acid-binding protein 1. This protein is responsible for the delivery of Retinoic Acid (RA) to its receptors thereby regulating its bioavailability and metabolism [ 24 , 25 ]. RA is important for the development of a healthy placenta by regulating the function of trophoblast placental cells [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients

Norton

Clarke

Holmstrom

et al. 2023

Cells

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Intrauterine growth restriction (IUGR) and preeclampsia (PE) are placental pathologies known to complicate pregnancy and cause neonatal disorders. To date, there is a limited number of studies on the genetic similarity of these conditions. DNA methylation is a heritable epigenetic process that can regulate placental development. Our objective was to identify methylation patterns in placental DNA from normal, PE and IUGR-affected pregnancies. DNA was extracted, and bisulfite was converted, prior to being hybridized for the methylation array. Methylation data were SWAN normalized and differently methylated regions were identified using applications within the USEQ program. UCSC’s Genome browser and Stanford’s GREAT analysis were used to identify gene promoters. The commonality among affected genes was confirmed by Western blot. We observed nine significantly hypomethylated regions, two being significantly hypomethylated for both PE and IGUR. Western blot confirmed differential protein expression of commonly regulated genes. We conclude that despite the uniqueness of methylation profiles for PE and IUGR, the similarity of some methylation alterations in pathologies could explain the clinical similarities observed with these obstetric complications. These results also provide insight into the genetic similarity between PE and IUGR and suggest possible gene candidates plausibly involved in the onset of both conditions.

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Section: Resultsmentioning

confidence: 99%

Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients

Norton

Clarke

Holmstrom

et al. 2023

Cells

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“…Patients with low serum retinol concentrations have less cellular retinoic acid availability and lower CRABP1 concentrations. In addition, there is an association between the concentration of CRABP2 and CRABP1; it has been observed that when there is a low synthesis of the CRABP2 protein, the expression of CRABP1 is suppressed (Enikeev et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Identificación de factores asociados al aumento de la expresión génica de CRABP1 en pacientes con lesiones intraepiteliales escamosas y cáncer de cuello uterino

Arellano-Ortiz,

Jiménez-Vega,

Salcedo-Vargas

et al. 2023

TIP RECQB

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La unión celular de la proteína con el ácido retinoico-1 (CRABP1) facilita el metabolismo de los retinoides y de su expresión anormal podría resultar en diversos cánceres. Para el caso que nos ocupa se evaluaron muestras de las biopsias de pacientes con cáncer de cuello uterino (CC) y con lesiones intraepiteliales escamosas de alto (HSIL) y de bajo grado (LSIL) (n= 66), por lo que para realizar el estudio se obtuvo la información clínico-patológica de las mujeres. La expresión del gen CRABP1 se examinó mediante RT-PCR y la proteína por inmunohistoquímica (IHC). La expresión de CRABP1 resultó ser más baja en el grupo HSIL significativamente (p = 0.008). La expresión proteica de CRABP1 se observó en el 90.3% de los tejidos, y en el CC su ausencia fue de un 9.7%. El aumento de la expresión génica, así como la disminución de la cantidad de proteína en este tipo de cáncer varía en diferentes situaciones médicas de acuerdo a lo observado: en el sobrepeso/obesidad aumenta la expresión génica (p= 0.019), pero no en la proteína (p = 0.053). Además, se mostró un aumento de la expresión CRABP1 con una concentración alta de retinol (p= 0.047) y la presencia de HVP-16 (p= 0.011) (expresión génica pero no la proteína, p > 0.05). Pacientes con menopausia se asociaron con la ausencia/débil inmunotinción de la proteína CRABP1 (p= 0.008). Finalmente, concluimos que CRABP1 y los factores asociados pueden ser útiles como biomarcadores para rastrear la progresión y la persistencia del cáncer de cuello uterino en el tejido.

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“…Our data revealed that CRABP2 was directly targeted by miR‐486‐3p, and circ_0011298 sponged miR‐486‐3p to upregulate CRABP2 expression. Many researches have suggested that CRABP2 is involved in regulation of the drug sensitivity in several cancers 31‐33 . In addition, Favorskaya et al reported that CRABP2 mRNA level was elevated in NSCLC samples 34 .…”

Section: Discussionmentioning

confidence: 99%

“…Many researches have suggested that CRABP2 is involved in regulation of the drug sensitivity in several cancers. 31 , 32 , 33 In addition, Favorskaya et al reported that CRABP2 mRNA level was elevated in NSCLC samples. 34 Moreover, Kim et al proved that plasma CRABP2 level was increased in NSCLC patients.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_0011298 enhances Taxol resistance of non‐small cell lung cancer by regulating miR‐486‐3p/CRABP2 axis

Xie

Wang

et al. 2022

Clinical Laboratory Analysis

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Background: Circular RNAs (circRNAs) serve as critical regulators in the chemoresistance of human cancers, including non-small cell lung cancer (NSCLC). We aimed to explore the role of hsa_circ_0011298 (circ_0011298) and its mechanism in Taxol resistance of NSCLC. : Circ_0011298, microRNA-486-3p (miR-486-3p), and CRABP2 mRNA expression were determined using qRT-PCR. EdU and MTT assays were used to detect cell proliferation. Cell cycle distribution and cell apoptosis were detected by flow cytometry. Cell migratory and invasive abilities were detected using transwell assay. Cellular glycolysis was determined by specific kits. Protein levels were examined by western blot. Dual-luciferase reporter and RIP assays were performed to confirm the relationship between miR-486-3p and circ_0011298 or CRABP2. Xenograft mice model was established to confirm the function of circ_0011298 in vivo. Results: Circ_0011298 was overexpressed in Taxol-resistant NSCLC cells and tissues. Circ_0011298 knockdown enhanced Taxol sensitivity by decreasing cell proliferation, migration, invasion, and glycolysis and inducing apoptosis and cell cycle arrest in Taxolresistant NSCLC cells. Circ_0011298 was a sponge of miR-486-3p, and the impact of circ_0011298 silencing on Taxol resistance was rescued by miR-486-3p inhibition. Moreover, miR-486-3p directly targeted CRABP2, and miR-486-3p inhibited Taxol resistance by targeting CRABP2. Furthermore, circ_0011298 regulated CRABP2 expression through targeting miR-486-3p. Importantly, circ_0011298 interference elevated Taxol sensitivity of NSCLC in vivo. Conclusion: Circ_0011298 elevated Taxol resistance of NSCLC by sponging miR-486-3p and upregulating CRABP2, providing a possible circRNA-targeted therapy for NSCLC. Methods

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CRABP1 and CRABP2 Protein Levels Correlate with Each Other but Do Not Correlate with Sensitivity of Breast Cancer Cells to Retinoic Acid

Cited by 9 publications

References 44 publications

Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients

Altered Epigenetic Profiles in the Placenta of Preeclamptic and Intrauterine Growth Restriction Patients

Identificación de factores asociados al aumento de la expresión génica de CRABP1 en pacientes con lesiones intraepiteliales escamosas y cáncer de cuello uterino

Circular RNA hsa_circ_0011298 enhances Taxol resistance of non‐small cell lung cancer by regulating miR‐486‐3p/CRABP2 axis

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