A novel tool for damage detection of an engineering structure based on the load path analysis using U * index is developed with high sensitivity. U * is a math index presentation of the internal stiffness distribution of a structure subjected to a certain load. The global variation of the stiffness distribution will occur due to the damage and can be measured by U * index as a new damage detection indicator. U * index theory is introduced first in the paper, and the damage detection mechanism and feasibility are then described, explained and studied through two sample structures by finite element analysis (FEA). Through the case studies, it is noted that good damage sensitivity can be realized even when the measurement position is far (30% of the whole structure size) away from the damage. The experimental study further validates the effectiveness and feasibility of U * measurement in damage detection. The U * index can be used as a new indicator for structural damage detection at an arbitrary measurement location with higher sensitivity than the stress analysis, which is only sensitive when the testing point is very close to the damage.INDEX TERMS U * index theory, load path analysis, static measurement, damage detection.