In recent years, the environmental evaluation of biorefineries has become critical for ensuring sustainable practices in bio-based production systems. This study focuses on the application of the Waste Reduction (WAR) Algorithm to assess the environmental impacts of an Extractive-based Creole-Antillean Avocado Biorefinery located in Northern Colombia, aimed at producing bio-oil, chlorophyll, and biopesticide from avocado pulp, peel, and seed, respectively. The environmental impacts were evaluated using the WAR algorithm, which quantifies the potential environmental impacts (PEI) of different process streams. The following four scenarios were developed: (1) considering only waste, (2) including waste and products, (3) including waste and energy sources, and (4) incorporating waste, products, and energy consumption. This study analyzed global impacts focusing on atmospheric and toxicological categories, with a detailed assessment of the most critical scenario. The results indicated that Scenario 4 had the highest PEI, particularly in the atmospheric and toxicological categories, driven by emissions of volatile organic compounds (VOCs), greenhouse gases (GHGs), and the presence of heavy metals. However, the avocado biorefinery process demonstrated a net reduction in overall environmental impacts, with negative PEI generation rates across all scenarios, suggesting that the biorefinery transforms high-impact substances into products with lower global impact potential. Energy consumption emerged as a significant contributor to environmental impacts, particularly in acidification potential (AP) and Atmospheric Toxicity Potential (ATP). Using natural gas as an energy source had a relatively lower environmental impact compared to coal and liquid fuels, emphasizing the need to optimize energy use in biorefinery design to improve environmental performance.