Cranberry cocktail juice, cranberry concentrates, and proanthocyanidins reduce reovirus infectivity titers in African green monkey kidney epithelial cell cultures

Abstract

Studies were performed to investigate the effect of several cranberry and grape juice extracts on the inhibition of reovirus infectivity following cell culture inoculation. Infectivity testing was performed utilizing cranberry juice extracts NutriCran-100 and NutriCran-90. At 5% extract concentrations, titers were reduced by ca. 50%. Cranberry cocktail juice caused an infectivity loss of ca. 10%. We ascribe these data to higher concentrations of proanthocyanidins (PACs) in the cranberry extracts. Further testing was performed utilizing purified high and low molecular weight cranberry PAC fractions (CB HMW and CB LMW, respectively), a cranberry flavonol glycoside (CB EToAc), cranberry anthocyanins (CB CA), and a grape PAC extract. Reovirus titers were reduced to undetectable levels at PAC concentrations < or =0.2%. CB CA had no effect on the inhibition of infectivity titers. Loss of infectivity titers was in the order: GP PAC>CB HMW>CB LMW>CB EToAc. Probe homogenization of CB HMW enhanced the extract to efficacy levels equal to that of grape PAC. Reovirus dsRNA segments were undetectable 96-h postcranberry cocktail juice pretreatment of MA-104 cell cultures. This study indicates an inhibition of reovirus infectivity titers by cranberry or grape juices or their purified PAC extracts. Viral inhibition probably occurs at the host cell surface.

Time-Dependent Effects of Pomegranate Juice and Pomegranate Polyphenols on Foodborne Viral Reduction

“…Recently, natural antimicrobials such as plant extracts have become increasingly popular options for viral reduction (Nair et al, 1999(Nair et al, , 2002Schlesinger et al, 2003;Weiss et al, 2005;Lipson et al, 2007a;Su et al, 2010a). The advantages for the use of natural plant antimicrobials include the absence of reported/observed toxic effects along with associated health benefits such as antioxidant, anticancer, anti-inflammatory, and antimicrobial properties (Prashanth et al, 2001;Negi and Jayaprakasha, 2003;Braga et al, 2005;Neurath et al, 2005;Pantuck et al, 2006;Miguel et al, 2007;Reddy et al, 2007;Haidari et al, 2009;Kasimsetty et al, 2009;Lee et al, 2010).…”

Anti-Influenza Virus Activity of Two Extracts of the Blackcurrant (Ribes Nigrum L.) From New Zealand and Poland

“…Differences in the effects on IFVAH1pdm and IFV -AH1tam r , which possess similar HA1, indicate the former possibility. A previous report also showed that the pretreatment of cell cultures with cranberry juice could reduce reovirus infectivity 4) . However, the different effects of blackcurrant on the adsorption of the 4 IFV strains to the same MDCK cell line, support latter possibility as well.…”

“…For instance, cranberries can prevent gastrointestinal disorders associated with Helicobacter pylori 1 -3) , reovirus 4) , rotavirus 5) and influenza virus 6) infections, and elderberry inhibits the replication of influenza virus 7) and ease its symptoms 8) . Blackcurrant (Ribes nigrum L.) is also known to inhibit the growth of herpes simplex virus type1 9) , the growth and release of influenza virus type A and B 10,11) .…”

Separation of Delphinidin-3-O-sambubioside, Cyanidin-3-O-sambubioside and p-Coumaric Acid from Cranberry by CCC Followed by prep-HPLC Using Robotic CCC Solvent System Selection

“…Phenolics and flavonoids present in the cranberry are known to provide antimicrobial and antioxidant activity [2,3,5] and they have cardioprotective and anticarcinogenic effects on human health [6][7][8]. Cranberry extracts are considered to exhibit antiviral and antibacterial activities [9][10][11][12][13] and cranberry juice and extract to inhibit the development of human breast cancer [7,14]. Proanthocyanidines present in the cranberry products have A-type linkage, while proanthocyanidines present in the other products have B-type linkage.…”