Cranberry proanthocyanidin-chitosan hybrid nanoparticles as a potential inhibitor of extra-intestinal pathogenic Escherichia coli invasion of gut epithelial cells

Alfaro‐Viquez, Emilia; Esquivel‐Alvarado, Daniel; Madrigal‐Carballo, Sergio; Krueger, Christian G.; Reed, Jess D.

doi:10.1016/j.ijbiomac.2018.01.033

Cited by 36 publications

(20 citation statements)

References 31 publications

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“…There are numerous studies describing the encapsulation of different extracts in chitosan nanoparticles via, mainly, the ionotropic gelation method with TPP, and their potential applications ( Table 1 ) [ 122 , 127 , 160 , 161 , 162 , 163 , 164 , 165 ]. Beconcini et al successfully encapsulated Crognola capannile cherry fruit extract ( Prunus avium L.), in two types of chitosan derivatives.…”

Section: Chitosan As a Matrix For The Encapsulation Of Plant Extramentioning

confidence: 99%

“…In another study, it was revealed that loading a blueberry-derived mixture of anthocyanins in CSNPs slowed the breakdown of anthocyanins in simulated gastrointestinal fluid and improved their stability [ 168 ]. Furthermore, in the study of Alfaro-Viquez and coworkers, a cranberry proanthocyanidins extract from the plant Vaccinium macrocarpon was encapsulated in chitosan, leading to increased stability, as well as molecular adhesion to extra-intestinal pathogenic Escherichia coli [ 164 ].…”

Section: Chitosan As a Matrix For The Encapsulation Of Plant Extramentioning

confidence: 99%

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Nanosystems for the Encapsulation of Natural Products: The Case of Chitosan Biopolymer as a Matrix

et al. 2020

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Chitosan is a cationic natural polysaccharide, which has emerged as an increasingly interesting biomaterialover the past few years. It constitutes a novel perspective in drug delivery systems and nanocarriers’ formulations due to its beneficial properties, including biocompatibility, biodegradability and low toxicity. The potentiality of chemical or enzymatic modifications of the biopolymer, as well as its complementary use with other polymers, further attract the scientific community, offering improved and combined properties in the final materials. As a result, chitosan has been extensively used as a matrix for the encapsulation of several valuable compounds. In this review article, the advantageous character of chitosan as a matrix for nanosystemsis presented, focusing on the encapsulation of natural products. A five-year literature review is attempted covering the use of chitosan and modified chitosan as matrices and coatings for the encapsulation of natural extracts, essential oils or pure naturally occurring bioactive compounds are discussed.

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Section: Chitosan As a Matrix For The Encapsulation Of Plant Extramentioning

confidence: 99%

Section: Chitosan As a Matrix For The Encapsulation Of Plant Extramentioning

confidence: 99%

Nanosystems for the Encapsulation of Natural Products: The Case of Chitosan Biopolymer as a Matrix

et al. 2020

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“…Many reports describe the encapsulation of various herbal extracts in CSNPs and their potential applications [34]. The encapsulation of plant extracts, such as garlic [35], cherry [36], green tea [37], tea polyphenols [38], cranberry [39], Arrabidaea chica extract [40], flavonoids [41], phenolic compounds [42], and Tridax procumbens leaf extract [43] in CSNPs has been outlined. However, to the best of our knowledge, there is no study on different characteristics of CSNPs containing the extract of pomegranate peels.…”

Section: Introductionmentioning

confidence: 99%

Chitosan Nanoparticles as a Promising Nanomaterial for Encapsulation of Pomegranate (Punica granatum L.) Peel Extract as a Natural Source of Antioxidants

et al. 2021

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The encapsulation of pomegranate peel extract (PPE) in chitosan nanoparticles (CSNPs) is an advantageous strategy to protect sensitive constituents of the extract. This study was aimed to develop PPE-loaded CSNPs and characterize their physical, structural morphology, antioxidant and antimicrobial properties. Spherical NPs were successfully synthesized with a mean diameter of 174–898 nm, a zeta potential (ZP) of +3–+36 mV, an encapsulation efficiency (EE) of 26–70%, and a loading capacity (LC) of 14–21% depending on their loaded extract concentrations. Based on these results, CSNPs with chitosan:PPE ratio of 1:0.50 (w/w) exhibited good physical stability (ZP = 27 mV), the highest loading (LC = 20%) and desirable encapsulation efficiency (EE = 51%), and thus, selected as optimally loaded NPs. The FTIR analysis of PPE-CSNPs demonstrated no spectral changes indicating no possible chemical interaction between the PPE and CSNPs, which confirms that the PPE was physically entrapped within NPs. Moreover, FTIR spectra of pure PPE showed specific absorption bands (at 3293–3450 cm−1) attributed to the incidence of phenolic compounds, such as tannic acid, ellagic acid and gallic acid. Total phenolic content (TPC) and antioxidant analysis of selected CSNPs revealed that the encapsulated NPs had significantly lower TPC and antioxidant activity than those of pure PPE, indicating that CSNPs successfully preserved PPE from rapid release during the measurements. Antibacterial tests indicated that pure PPE and PPE-loaded CSNPs effectively retarded the growth of Gram-positive S. aureus with a minimum inhibitory concentration (MIC) of 0.27 and 1.1 mg/mL, respectively. Whereas Gram-negative E. coli, due to its protective cell membrane, was not retarded by pure PPE and PPE-CSNPs at the MIC values tested in this study. Gas chromatography-mass spectroscopy analysis confirmed the incidence of various phytochemicals, including phenolic compounds, fatty acids, and furfurals, with possible antioxidant or antimicrobial properties. Overall, CSNPs can be regarded as suitable nanomaterials for the protection and controlled delivery of natural antioxidants/antimicrobials, such as PPE in food packaging applications.

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“…Chitosan NPs loaded with proanthocyanidins have also been proposed to inhibit pathogenic bacteria in the intestine (Alfaro-Viquez, Esquivel-Alvarado, Madrigal-Carballo, Krueger & Reed, 2018). The extra-intestinal pathogenic bacteria chosen in this study were Escherichia coli, and in vitro inhibition was carried out on intestinal epithelial cells, showing that loaded NPs were as inhibitory as free proanthocyanidins.…”

Section: Colonic Pathogenic Bacteria Inhibitionmentioning

confidence: 91%

Enhancement of oral bioavailability of natural compounds and probiotics by mucoadhesive tailored biopolymer-based nanoparticles: A review

Gómez‐Guillén

Montero

2021

Food Hydrocolloids

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Encapsulation in polymer NPs (NPs) is a well-recognized way to improve the oral bioavailability of hydrophobic and hydrophilic compounds by enhancing their solubility in aqueous medium, to protect them from acid and enzymatic degradation in the gastrointestinal tract, and to favour their permeability across the mucosal membranes. Nanostructures rather than larger delivery systems are also proposed to encapsulate prebiotics, probiotics and natural antimicrobial compounds, as they allow greater penetration of the mucous layer, as well as lower and longer the delivery of prebiotics, probiotics and antimicrobials along the gastrointestinal tract. This also includes a previous brief introduction to the complex mucoadhesion mechanism and common evaluation methods for determining mucoadhesiveness in nanoparticulate systems.

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Cranberry proanthocyanidin-chitosan hybrid nanoparticles as a potential inhibitor of extra-intestinal pathogenic Escherichia coli invasion of gut epithelial cells

Cited by 36 publications

References 31 publications

Nanosystems for the Encapsulation of Natural Products: The Case of Chitosan Biopolymer as a Matrix

Nanosystems for the Encapsulation of Natural Products: The Case of Chitosan Biopolymer as a Matrix

Chitosan Nanoparticles as a Promising Nanomaterial for Encapsulation of Pomegranate (Punica granatum L.) Peel Extract as a Natural Source of Antioxidants

Enhancement of oral bioavailability of natural compounds and probiotics by mucoadhesive tailored biopolymer-based nanoparticles: A review

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