Cranberry Proanthocyanidins Inhibit MMP Production and Activity

La, Vu Dang; Howell, Amy B.; Grenier, Daniel

doi:10.1177/0022034509339487

Cited by 90 publications

(72 citation statements)

References 36 publications

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“…In the present study, we showed that AC-PACs inhibited collagen degradation by P. gingivalis in a dose-dependent manner, suggesting that ACPACs may contribute to reducing the destructive process. Interestingly, we previously showed that AC-PACs have the ability to inhibit the activity of recombinant human matrix metalloproteinases (MMP-1 and MMP-9) and the secretion of various MMPs by macrophages (29). Using a reconstituted basement membrane model composed of laminin, type IV collagen, proteoglycans, and entactin, we also showed that AC-PACs could inhibit the invasiveness of P. gingivalis.…”

Section: Discussionmentioning

confidence: 84%

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Anti- Porphyromonas gingivalis and Anti-Inflammatory Activities of A-Type Cranberry Proanthocyanidins

Howell

Grenier

2010

Antimicrob Agents Chemother

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A-type cranberry proanthocyanidins (AC-PACs) have recently been reported to be beneficial for human health, especially urinary tract health. The effect of these proanthocyanidins on periodontitis, a destructive disease of tooth-supporting tissues, needs to be investigated. The purpose of this study was to investigate the effects of AC-PACs on various virulence determinants of Porphyromonas gingivalis as well as on the inflammatory response of oral epithelial cells stimulated by this periodontopathogen. We examined the effects of AC-PACs on P. gingivalis growth and biofilm formation, adherence to human oral epithelial cells and proteincoated surfaces, collagenase activity, and invasiveness. We also tested the ability of AC-PACs to modulate the P. gingivalis-induced inflammatory response by human oral epithelial cells. Our results showed that while AC-PACs neutralized all the virulence properties of P. gingivalis in a dose-dependent fashion, they did not interfere with growth. They also inhibited the secretion of interleukin-8 (IL-8) and chemokine (C-C motif) ligand 5 (CCL5) but did not affect the secretion of IL-6 by epithelial cells stimulated with P. gingivalis. This anti-inflammatory effect was associated with reduced activation of the nuclear factor-B (NF-B) p65 pathway. AC-PACs may be potentially valuable bioactive molecules for the development of new strategies to treat and prevent P. gingivalis-associated periodontal diseases.

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Section: Discussionmentioning

confidence: 84%

“…To investigate the effect of AC-PACs on various pathogenic properties of P. gingivalis, up to 100 g/ml of AC-PACs was used, since this concentration has been reported to be nontoxic for human cells (29). Biofilm formation by P. gingivalis was significantly decreased by 50 and 100 g/ml AC-PACs.…”

Section: Resultsmentioning

confidence: 99%

Anti- Porphyromonas gingivalis and Anti-Inflammatory Activities of A-Type Cranberry Proanthocyanidins

Howell

Grenier

2010

Antimicrob Agents Chemother

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“…A utilização de PAs visando à inibição de proteases tem sido investigada não somente buscando a estabilidade da união resina-dentina, mas também para a prevenção e a redução de câncer 20 , e controle da progressão de doenças periodontais 15,21 . As MMPs são proteases zinco/cálcio dependentes que necessitam de íons cálcio para manter sua estrutura terciária, bem como de íons zinco para catalisar o processo de hidrólise.…”

Section: Discussionunclassified

“…A prolina aumenta a especificidade da interação com as PAs, favorecendo a formação de ligações de hidrogênio entre a carbonila da amida proteica com a hidroxila fenólica 14 . Além disso, tem sido demonstrado que as PAs também desempenham um papel importante tanto na inibição da produção das MMPs -1, -3, -7, -8, -9 e -13 liberadas pelos macrófagos quanto na atividade catalítica das MMPs -1 e -9 15,16 . Estudos anteriores têm relatado que a aplicação de PA (extraída de semente de uva) na dentina desmineralizada apresenta resultados significativos tanto nas propriedades mecânicas como na resistência de união da interface adesiva após 10 e 60 minutos de tratamento.…”

Section: Introductionunclassified

Redução da atividade proteolítica da dentina após curtos períodos de aplicação de proantocianidina

Delgado

Scheffel

et al. 2015

Rev. odontol. UNESP

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ResumoIntrodução: Agentes promotores de ligações cruzadas têm sido investigados como inibidores da atividade enzimática da dentina, o que favoreceria a longevidade das restaurações adesivas. Objetivo: Avaliar o efeito do tratamento da dentina com proantocianidina (PA), em curtos períodos de tempo, na inibição da atividade de MMPs in situ. Material e método: Quarenta espécimes de dentina (1×1×6 mm) foram obtidos de molares hígidos e divididos em quatro grupos (n=10). Os espécimes foram condicionados com ácido fosfórico por 15 s, seguido de lavagem em água deionizada. A dentina condicionada foi tratada com: água, 5% PA por 5 s, 15 s ou 30 s. A atividade de MMP foi analisada colorimetricamente (SensoLyte®) e os dados de absorbância (412 nm) foram submetidos aos testes de ANOVA e Tukey (α=0,05). Resultado: Todos os períodos de tratamento foram capazes de reduzir a atividade de MMPs, sendo que os melhores resultados foram observados para a dentina tratada com PA por 15 s (63,1% redução) e 30 s (70,2%). O tratamento por 5 s foi capaz de inibir 39,9% das MMPs. Conclusão: A aplicação de PA sobre a dentina condicionada foi capaz de reduzir a atividade de MMPs mesmo em períodos de tempo extremamente curtos, como 5 s. No entanto, melhores resultados foram obtidos com os maiores períodos de tratamento.Descritores: Dentina; colágeno; metaloproteinase da matriz; proantocianidina. AbstractIntroduction: Collagen cross-linkers have been investigated as inhibitors of the enzymatic activity of dentin, therefore improving the longevity of adhesive restorations. Purpose: To evaluate the effect of etched dentin treatment with proanthocyanidin (PA) in short periods of time on the inhibition of dentin metalloproteinases (MMP) activity in situ. Material and method: Forty dentin specimens (1x1x6mm) were obtained from sound third molars and divided into 4 groups (n=10). The specimens were etched with 37% phosphoric acid for 15s and rinsed in deionized water. Then they were treated with the following solutions: water, 5% PA for 5s, 15s or 30s. The total MMP activity was analyzed by a colorimetric test (SensoLyte®). Absorbance data (412nm) were submitted to ANOVA and Tukey tests (α=0.05). Result: All treatment periods were able to reduce the total activity of MMPs. The best results were observed for dentine treated with PA for 15s (63.1% reduction) and 30s (70.2%). Treatment for 5s was capable of inhibiting only 39.9% of the total MMP activity. Conclusion: Application of PA on the etched dentin in extremely short periods of time reduced the MMPs activity of dentin, even after 5s. However, the best results were obtained for the longer periods.

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“…In addition to create covalent cross-links in collagen, cross-linkers are capable to cross-link the protease enzymes directly and interfere with their molecular mobility 65) . The other supposed paradigm is that PA may indirectly interfere with production and activation process of proteases by modulating host immune responses 74) . There are some reports about PA MMP inhibitory effects somewhere in human body aside from dentin, as the case of point, cranberry PA can inhibit production and secretion of MMP-1 and MMP-9 by macrophages in response to periodontal pathogens 75) .…”

Section: Pa-dentinal Matrix Metalloproteinase Interactionsmentioning

confidence: 99%

Dual function of proanthocyanidins as both MMP inhibitor and crosslinker in dentin biomodification: A literature review

Balalaie

Rezvani

Basir

2018

Dental Materials Journal

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Proanthocyanidin, a natural phytochemical bioactive agent, simultaneously can silence the activity of dentinal proteases and crosslink the collagen matrix; both of these phenomena would be the fundamentals for bio-stability of resin-dentin interface which is essential for a promising adhesive dentistry. This review provides an overview of the data developed by different groups of researchers and highlighted topics are proanthocyanidin chemistry, natural resources and the unique interactions between proanthocyanidincollagen and proanthocyanidin-MMPs in dentin. Besides, clinical applications of proanthocyanidin in the form of proanthocyanidincontaining adhesives, preconditioners and etchants have been reviewed. One hundred and twelve studies have been published in peer-reviewed journals from 1981 to 2017, all were comprised in this review, some of them have been actually proven to be promising from clinical point of view and others need further assessment before their adoption as clinically practicable protocols.

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Cranberry Proanthocyanidins Inhibit MMP Production and Activity

Cited by 90 publications

References 36 publications

Anti- Porphyromonas gingivalis and Anti-Inflammatory Activities of A-Type Cranberry Proanthocyanidins

Anti- Porphyromonas gingivalis and Anti-Inflammatory Activities of A-Type Cranberry Proanthocyanidins

Redução da atividade proteolítica da dentina após curtos períodos de aplicação de proantocianidina

Dual function of proanthocyanidins as both MMP inhibitor and crosslinker in dentin biomodification: A literature review

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