Crayfish hemocytes develop along the granular cell lineage

Li, Fang; Zheng, Zaichao; Li, Hongyu; Fu, Rongrong; Xu, Limei; Yang, Feng

doi:10.1038/s41598-021-92473-9

Cited by 22 publications

(28 citation statements)

References 59 publications

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“…In our short-term in vitro experiments, nuclear BrdU labeling was evident only in the hyaline cells, which were quite numerous, in contrast to reports in other species (e.g., Li et al., 2021 ). Nuclear BrdU was found in 10–15% of hyaline cells that were identified, suggesting that this population is active in the cell cycle ( Figures 4 A–4C).…”

Section: Resultscontrasting

confidence: 99%

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Adult neurogenesis in crayfish: Identity and regulation of neural progenitors produced by the immune system

Benton

Weisbach

et al. 2022

iScience

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Section: Resultscontrasting

confidence: 99%

“…It has been reported that circulating hemocytes in crayfish proliferate rarely ( Bauchau, 1981 ; Söderhäll et al., 2003 ; Li et al., 2021 ). We wanted to test this in P. clarkii , because this has important implications for these studies.…”

Section: Resultsmentioning

confidence: 99%

Adult neurogenesis in crayfish: Identity and regulation of neural progenitors produced by the immune system

Benton

Weisbach

et al. 2022

iScience

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“…Another reason for the change in the proportions of the hemocyte types might also be the conversion between the main hemocyte types (e.g., granule accumulation or degranulation). For instance, some studies have indicated that the different types of hemocytes represent different stages of hemocyte development ( 54 , 55 ).…”

Section: Discussionmentioning

confidence: 99%

Time-Course of the Innate Immune Response of the Terrestrial Crustacean Porcellio scaber After Injection of a Single Dose of Lipopolysaccharide

2022

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Invertebrates, including crustaceans, rely on cellular and humoral immune responses to protect against extrinsic and intrinsic factors that threaten their integrity. Recently, different immune parameters have been increasingly used as biomarkers of effects of pollutants and environmental change. Here, we describe the dynamics of the innate immune response of the terrestrial crustacean Porcellio scaber to injection of a single dose of lipopolysaccharide (LPS), an important molecular surface component of the outer membrane of Gram-negative bacteria. The aim was to provide a basis for interpretation of change in immune parameters as a result of different challenges, including microplastics and nanoplastics exposure. Changes in total and differential numbers of hemocytes, hemocyte viability, and humoral immune parameters (i.e., phenoloxidase-like activity, nitric oxide levels) were assessed at different times (3, 6, 12, 24, 48 h). An injection of 0.5 μg/μL LPS into the body of P. scaber resulted in a rapid decrease (3 h after LPS injection) in the total number of hemocytes and reduced viability of the hemocytes. This was accompanied by changed proportions of the different hemocyte types, as a decrease in the numbers of semigranulocytes and granulocytes, and a marked increase in the numbers of hyalinocytes. In addition, phenoloxidase-like activity and nitric oxide levels in the hemolymph were increased at 3 h and 6 h, respectively, after the LPS challenge. Forty-eight hours after LPS injection, the immune parameters in the hemolymph of P. scaber had returned to those before the LPS challenge. This suggests that the innate immune system successfully protected P. scaber from the deleterious effects of the LPS challenge. These data indicate the need to consider the dynamics of innate immune responses of P. scaber when effects of infections, pollutants, or environmental changes are studied. We also propose an approach to test the immunocompetence of organisms after different challenges in ecotoxicity studies, based on the dynamics of their immune responses.

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“…The origin of the hyaline cell is not yet resolved. Li et al (2021) recently proposed an alternative model of haematopoiesis for crayfish Cherax quadricarinatus in which the circulating haemocytes belong to one lineage. Semigranulocytes and granulocytes were interpreted as different developmental stages rather than two types of fully differentiated cells.…”

Section: ) E-cells Of the Hepatopancreasmentioning

confidence: 99%

Cytology, function and dynamics of stem and progenitor cells in decapod crustaceans

Vogt

2021

Biological Reviews

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Stem cells play key roles in development, tissue homeostasis, regeneration, ageing and diseases. Comprehensive reviews on stem cells are available for the determinately growing mammals and insects and some lower invertebrates like hydra but are rare for larger, indeterminately growing invertebrates that can live for many decades. This paper reviews the cytology, function and dynamics of stem and progenitor cells in the decapod crustaceans, a species‐rich and ecologically and economically important animal group that includes mainly indeterminate growers but also some determinate growers. Further advantages of decapods for stem cell research are almost 1000‐fold differences in body size and longevity, the regeneration of damaged appendages and the virtual absence of age‐related diseases and tumours in the indeterminately growing species. The available data demonstrate that the Decapoda possess a remarkable variety of structurally and functionally different stem cells in embryos and larvae, and in the epidermis, musculature, haematopoietic tissue, heart, brain, hepatopancreas, olfactory sense organs and gonads of adults. Some of these seem to be rather continuously active over a lifetime but others are cyclically activated and silenced in periods of days, weeks and years, depending on the specific organ and function. Stem cell proliferation is triggered by signals related to development, moulting, feeding, reproduction, injury, infection, environmental enrichment and social status. Some regulatory pathways have already been identified, including the evolutionarily conserved GATA‐binding and runt‐domain transcription factors, the widespread neurotransmitter serotonin, the arthropod‐specific hormone 20‐hydroxyecdysone and the novel astakine growth factors. Knowledge of stem cells in decapods primarily refines our picture on the development, growth and maintenance of tissues and organs in this animal group. Cultured decapod stem cells have good potential for toxicity testing and virus research with practical relevance for aquaculture. Knowledge of stem cells in decapods also broadens our understanding of the evolution of stem cells and regeneration in the animal kingdom. The stem cells of long‐lived, indeterminately growing decapods may hold the key to understanding how stem and progenitor cells function into old age without adverse side effects, possibly evoking new ideas for the development of anti‐ageing and anti‐cancer treatments in humans.

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Crayfish hemocytes develop along the granular cell lineage

Cited by 22 publications

References 59 publications

Adult neurogenesis in crayfish: Identity and regulation of neural progenitors produced by the immune system

Adult neurogenesis in crayfish: Identity and regulation of neural progenitors produced by the immune system

Time-Course of the Innate Immune Response of the Terrestrial Crustacean Porcellio scaber After Injection of a Single Dose of Lipopolysaccharide

Cytology, function and dynamics of stem and progenitor cells in decapod crustaceans

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