Crc Is Involved in Catabolite Repression Control of the
 bkd
 Operons of
 Pseudomonas putida
 and
 Pseudomonas aeruginosa

Hester, Kathryn L.; Lehman, J. J.; Najar, Fares Z.; Song, Lin; Roe, Bruce A.; MacGregor, C H; Hager, Paul W.; Phibbs, Paul V.; Sokatch, John R.

doi:10.1128/jb.182.4.1144-1149.2000

Cited by 99 publications

(103 citation statements)

References 33 publications

(22 reference statements)

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“…Inactivation of the crc gene relieved the repression of a number of genes implicated in the metabolism of carbohydrates and nitrogenated compounds in Pseudomonas aeruginosa and P. putida (Collier et al, 1996;Hester et al, 2000a).…”

Section: Operons Affected By the Repression In A Baylyimentioning

confidence: 99%

Aromatic degradative pathways in Acinetobacter baylyi underlie carbon catabolite repression

2008

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Carbon catabolite repression is an important mechanism allowing efficient carbon source utilization. In the soil bacterium Acinetobacter baylyi, this mechanism has been shown to apply to the aromatic degradative pathways for the substrates protocatechuate, p-hydroxybenzoate and vanillate. In this investigation, transcriptional fusions with the gene for luciferase in the gene clusters for the degradation of benzyl esters, anthranilate, benzoate, hydroxycinnamates and dicarboxylates (are, ant, ben, hca and dca genes) were constructed and established in the chromosome of A. baylyi. The respective strains revealed the presence of strong carbon catabolite repression at the transcriptional level. In all cases, succinate and acetate in combination had the strongest repressing effect, and pyruvate (or lactate in case of the ben and hca genes) allowed the highest expression when these carbon sources were supplied together with the respective inducer. The pattern of repression for the different cosubstrates was similar for all operons investigated and was also observed in the absence of the respective inducing compounds, indicating a mechanism that is independent of the respective specific regulators. Repression by acetate and succinate varied between 88 % for the hca genes and 99 % for the pca genes.

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Section: Operons Affected By the Repression In A Baylyimentioning

confidence: 99%

Aromatic degradative pathways in Acinetobacter baylyi underlie carbon catabolite repression

2008

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“…1 In nonenteric Gram-negative bacteria, such as Pseudomonas, general induction of hut expression is not well understood. Succinate-induced catabolite repression (based solely on enzyme activity assays) has been reported (Hug et al 1968;Phillips and Mulfinger 1981), although no role has been found for the global regulatory proteins Crc (catabolic repression control; Hester et al 2000) and Vfr (virulence factor regulator, which is homologous to the E. coli CAP protein; Suh et al 2002). Recent work has shown the involvement of a newly discovered two-component regulatory system, CbrAB (Nishijyo et al 2001;Zhang and Rainey 2007).…”

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confidence: 99%

Dual Involvement of CbrAB and NtrBC in the Regulation of Histidine Utilization inPseudomonas fluorescensSBW25

Zhang

Rainey

2008

Genetics

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Pseudomonas fluorescens SBW25 is capable of growing on histidine as a sole source of carbon and/or nitrogen. Previous work showed that the two-component regulatory system CbrAB is required for expression of the histidine utilization (hut) locus when histidine is the sole source of carbon and nitrogen. Here, using mutational analysis and transcriptional assays, we demonstrate involvement of a second twocomponent system, NtrBC. When histidine is the sole carbon source, transcription of the hutU operon is initiated from a s 54 -type promoter and requires CbrB (an enhancer binding protein for s 54 -recruitment). However, when histidine is the sole nitrogen source, the hutU operon is transcribed from a s 70 -type promoter and requires either CbrB or the nitrogen regulator, NtrC. No role was found for the SBW25 homolog of the nitrogen assimilation control protein (NAC). Biolog phenotypic microarray analysis of the ability of the three mutants (DcbrB, DntrC, and DcbrB DntrC) to utilize 190 carbon and 95 nitrogen substrates confirmed the central regulatory roles of CbrAB and NtrBC in cellular carbon and nitrogen catabolism: deletion of cbrB abolished growth on 20 carbon substrates; deletion of ntrC eliminated growth on 28 nitrogen substrates. A double cbrB-ntrC mutant was unable to utilize a further 14 nitrogen substrates (including histidine, proline, leucine, isoleucine, and valine). Our data show that CbrAB plays a role in regulation of both carbon and nitrogen catabolism and maintains activity of catabolic pathways under different C:N ratios.

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“…The levels and activity of Crc vary depending on growth conditions [231] and it has also been reported to inhibit the expression of many other catabolic pathways for several non-preferred compounds in Pseudomonads [230,[232][233][234][235][236][237].…”

Section: Catabolite Repression Controlmentioning

confidence: 99%

Monooxygenases involved in the n-alkanes metabolism by Rhodococcus sp. BCP1: molecular characterization and expression of alkB gene

Cappelletti

Fedi

Honda

et al. 2010

Journal of Biotechnology

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Discussion 107 Summary 113Chapter 5 -Analysis of the alkB gene expression Summary 157Chapter 6 -Proteomic analysis of n-alkanes growth of Rhodococcus sp. BCP1 Chapter 1 -General Introduction PrefaceThe quality of life on Earth is tightly related with the overall quality of the environment [1]. During the last century it was commonly believed that the abundance of land and resources were unlimited and it was rarely acknowledged that the production, use, and disposal of hazardous substances had environmental and health effects [1][2][3]. Owing to this, human activity has deliberately or inadvertently released into waters and soil a variety of toxic compounds as refrigerants, paints, solvents, herbicides and pesticides that can cause considerable environmental pollution and human health problems as a result of their persistence, toxicity, and transformation into hazardous metabolites [4,5]. Very soon, however, it became clear how the carelessness and negligence in handling the industrial wastes and gas emissions had caused dramatic effect on the nature and on human health [1].Nowadays, the concern about the global warming and the climate changing induced the government regulatory agencies to establish procedures for assessing the environmental impact and health hazards of chemicals and for controlling/limiting their utilization.International efforts have been applied to remedy many contaminated sites all over the world, either as a response to the risk of adverse health or environmental effects caused by contamination or to enable the site to be redeveloped for use [5]. In response to public and government concern and because of the intriguing research problems presented, environmental scientists, biologists and chemists have been giving increased attention to identifying and determining the behavior and fate of organic compounds in natural ecosystems [5].Bioremediation procedures offer an economical and effective possibility to degrade or render innocuous toxic pollutants using natural biological activity [1]. By definition, bioremediation implies the use of living organisms, primarily microorganisms, to degrade the Since contaminant compounds are transformed by living organisms through reactions that take place as a part of their metabolic processes, environmental biotechnology focuses on the development of techniques to optimize environmental parameters to allow these metabolic pathways to proceed faster and, therefore, to improve the bio-degradation [1]. As a result, the removal of the contaminated soil can be necessary to let the biodegradation proceed in controlled sites (such as bioreactors). These ex situ approaches provide optimal conditions out one single step of the entire bio-degradation process. Hydrocarbons in the environmentHydrocarbons are energy-rich organic compounds consisting of carbon and hydrogen atoms and they can be saturated (only single C-H bounds) or unsaturated (one ore more double or triple C-H bounds). Alkanes are saturated hydrocarbons with the general formula C n H 2n+2 ; they can b...

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Crc Is Involved in Catabolite Repression Control of the bkd Operons of Pseudomonas putida and Pseudomonas aeruginosa

Cited by 99 publications

References 33 publications

Aromatic degradative pathways in Acinetobacter baylyi underlie carbon catabolite repression

Aromatic degradative pathways in Acinetobacter baylyi underlie carbon catabolite repression

Dual Involvement of CbrAB and NtrBC in the Regulation of Histidine Utilization inPseudomonas fluorescensSBW25

Monooxygenases involved in the n-alkanes metabolism by Rhodococcus sp. BCP1: molecular characterization and expression of alkB gene

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