Cre–lox recombination: Cre-ative tools for plant biotechnology

Gilbertson, Larry A.

doi:10.1016/j.tibtech.2003.09.011

Cited by 129 publications

(85 citation statements)

References 54 publications

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“…The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert DNA cassettes into eukaryotic chromosomes is widespread (1)(2)(3)(4)(5)(6)(7)(8)(9). Indeed, a PubMed search for "cre recombinase" in early 2014 returned over 4000 articles.…”

Section: Introductionmentioning

confidence: 99%

Cre Recombinase

Duyne

2015

Microbiol Spectr

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The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert DNA cassettes into eukaryotic chromosomes is widespread. In addition to the numerous in vivo and in vitro applications that have been reported since Cre was first shown to function in yeast and mammalian cells nearly 30 years ago, the Cre-loxP system has also played an important role in understanding the mechanism of recombination by the tyrosine recombinase family of site-specific recombinases. The simplicity of this system, requiring only a single recombinase enzyme and short recombination sequences for robust activity in a variety of contexts, has been an important factor in both cases. This review discusses advances in the Cre recombinase field that have occurred over the past 12 years since the publication of Mobile DNA II. The focus is on those recent contributions that have provided new mechanistic insights into the reaction. Also discussed are modifications of Cre and/or the loxP sequence that have led to improvements in genome engineering applications.

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Section: Introductionmentioning

confidence: 99%

Cre Recombinase

Duyne

2015

Microbiol Spectr

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“…The Cre/lox system is used to induce chromosomal inversions, translocations and deletions in planta (reviewed by Ow and Medberry (1995) and Gilbertson (2003)). …”

Section: Introductionmentioning

confidence: 99%

Size Does Matter: Cre-mediated Somatic Deletion Efficiency Depends on the Distance Between the Target lox-Sites

et al. 2005

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Cre/lox recombination in vivo has become an important tool to induce chromosomal rearrangements like deletions. Using a combination of Ds transposition and Cre/lox recombination in two independent experiments on chromosomes 6 and 7 of tomato, two sets of somatic deletions up to a size of 200 kb were obtained. The efficiency of somatic deletion decreased with increasing deletion size. The largest germinally transmitted deletion had a size of only 55 kb. The results show that Cre-mediated deletion in somatic cells is less efficient when the lox sites are separated over larger distances. A further drop of the deletion efficiency after germinal transmission of the larger deletions can be explained by the probable loss of genes that are of vital importance to gametophyte function. Plasmid rescue of an 8.4 kb circularised deleted DNA showed that the Cre-mediated deletion takes place in tomato as expected. Since the circular Cre-deleted DNA could only be PCR amplified in plant cells where the deletion was not complete, the double-stranded DNA circle is assumed to be instable.

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“…A diagram showing the insertion of the LY038 insert and nptII excision by cre/lox is presented in Figure 6-1. The nptII cassette was flanked by 2 loxP sites to allow for its subsequent removal by Cre recombinase (Gilbertson 2003). An extensive number of Southern blots, including generational stability analyses over multiple generations, established that there were no genetic elements from the cre and nptII coding sequences detected in Lysine maize.…”

Section: Crop Transformation and Breedingmentioning

confidence: 99%

Front Matter

2008

Comp Rev Food Sci Food Safe

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Cre–lox recombination: Cre-ative tools for plant biotechnology

Cited by 129 publications

References 54 publications

Cre Recombinase

Cre Recombinase

Size Does Matter: Cre-mediated Somatic Deletion Efficiency Depends on the Distance Between the Target lox-Sites

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