Creatine increases IGF‐I and myogenic regulatory factor mRNA in C<sub>2</sub>C<sub>12</sub> cells

Louis, Magali; Beneden, Ronald Van; Dehoux, Mischaël; Thissen, Jean Paul; Francaux, Marc

doi:10.1016/s0014-5793(03)01504-7

Cited by 72 publications

(63 citation statements)

References 28 publications

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“…IGF-I is known to stimulate proliferation, differentiation (Florini et al, 1996;Oksbjerg et al, 2004 and and protein synthesis in muscle cells (Hembree et al, 1991). Stimulation of muscle growth may be mediated by increased plasma IGF-I, which originates primarily from the liver or is produced locally in the muscle tissues (Louis et al, 2004;Deldicque et al, 2005). In this study, the expression of IGF-I gene was significantly increased in leg muscle in both the Cr-Pyr and Cr groups, and this was accompanied by increased relative leg muscle weight.…”

Section: Discussionmentioning

confidence: 53%

Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

Chen

Wang

Kong

et al. 2011

Animal

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The effects of pyruvate (Pyr), creatine pyruvate (Cr-Pyr) and creatine (Cr) on lipid and protein metabolism were compared in broiler chickens. A total of 400 1-day-old male birds (Aconred) were allocated to four groups, each of which included four replicates (25 birds per replicate). Treatments consisted of unsupplemented basal diet (Control), basal diet containing 2% Pyr, basal diet containing 3% Cr and basal diet containing 5% Cr-Pyr. Cr-Pyr and Pyr significantly decreased the hepatic triglyceride and serum total cholesterol concentration (P , 0.01). Cr-Pyr markedly increased the serum non-esterified fatty acid and highdensity lipoprotein cholesterol concentrations (P , 0.05), whereas the expression of carnitine palmitoyl transferase I (P , 0.05) and peroxisome proliferators-activated receptor-a (P , 0.01) mRNA in the liver were both decidedly enhanced in the Cr-Pyr group. The relative leg muscle weight was higher in the Cr-Pyr group than in the control group, whereas the serum uric acid content and hepatic glutamic-oxaloacetic transaminase activity were lower in the Cr-Pyr and Cr groups (P , 0.05), respectively. Muscle insulin-like growth factor I (P , 0.05) expression was enhanced, and the myostatin (P , 0.01) mRNA level was reduced in both the Cr-Pyr and Cr groups. In addition, Cr-Pyr did not alter body weight or the feed conversion ratio. These results indicate that, compared with Pyr and Cr alone, Cr-Pyr has a bifunctional role in broiler chickens, in that it influences both lipid and protein metabolism.Keywords: creatine pyruvate, pyruvate, creatine, lipolysis, protein synthesis ImplicationsIn this study, the administration of creatine pyruvate (Cr-Pyr, a new compound, which contains pure pyruvic acid molecularly bound to pure pharmaceutical grade creatine at a concentration ratio of 40 : 60) was compared with the administration of Pyr or Cr alone for its effect on lipid and protein metabolism in broilers. The insights gained from this study will provide a better understanding of the mechanisms involved in the Cr-Pyr effect on broiler chickens. Further clarity will help the industry to avoid obesity-related problems during production and explore new ways to improve muscle growth in broilers.

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Section: Discussionmentioning

confidence: 53%

Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

Chen

Wang

Kong

et al. 2011

Animal

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“…The mechanisms by which creatine protected against hypoxia-induced muscle atrophy are unknown, and their identification were not within the scope of this study. However, creatine loading has been shown to increase growth factor signaling such as IGF-1 (35), which can suppress atrogin-1 and MuRF1 levels (36), a creatine-associated response observed in this study.…”

Section: Discussionmentioning

confidence: 60%

Maternal creatine supplementation from mid-pregnancy protects the newborn spiny mouse diaphragm from intrapartum hypoxia-induced damage

et al. 2010

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ABSTRACT:We hypothesized that maternal creatine supplementation from mid-pregnancy would protect the diaphragm of the newborn spiny mouse from the effects of intrapartum hypoxia. Pregnant mice were fed a control or 5% creatine-supplemented diet from mid-gestation. On the day before term, intrapartum hypoxia was induced by isolating the pregnant uterus in a saline bath for 7.5-8 min before releasing and resuscitating the fetuses. Surviving pups were placed with a cross-foster dam, and diaphragm tissue was collected at 24 h postnatal age. Hypoxia caused a significant decrease in the cross-sectional area (ϳ19%) and contractile function (26.6% decrease in maximum Ca 2ϩ -activated force) of diaphragm fibers. The mRNA levels of the muscle mass-regulating genes MuRF1 and myostatin were significantly increased (2-fold). Maternal creatine significantly attenuated hypoxia-induced fiber atrophy, contractile dysfunction, and changes in mRNA levels. This study demonstrates that creatine loading before birth significantly protects the diaphragm from hypoxiainduced damage at birth. (Pediatr Res 68: 393-398, 2010)

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“…MyoD, Myf5, and myogenin primers and GAPDH and growth factor primers were designed as described previously (31,32).…”

Section: Real-time Polymerase Chainmentioning

confidence: 99%

Trpc1 Ion Channel Modulates Phosphatidylinositol 3-Kinase/Akt Pathway during Myoblast Differentiation and Muscle Regeneration

Zanou

Schakman

Louis

et al. 2012

Journal of Biological Chemistry

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Creatine increases IGF‐I and myogenic regulatory factor mRNA in C₂C₁₂ cells

Cited by 72 publications

References 28 publications

Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

Maternal creatine supplementation from mid-pregnancy protects the newborn spiny mouse diaphragm from intrapartum hypoxia-induced damage

Trpc1 Ion Channel Modulates Phosphatidylinositol 3-Kinase/Akt Pathway during Myoblast Differentiation and Muscle Regeneration

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Creatine increases IGF‐I and myogenic regulatory factor mRNA in C2C12 cells

Cited by 72 publications

References 28 publications

Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

Maternal creatine supplementation from mid-pregnancy protects the newborn spiny mouse diaphragm from intrapartum hypoxia-induced damage

Trpc1 Ion Channel Modulates Phosphatidylinositol 3-Kinase/Akt Pathway during Myoblast Differentiation and Muscle Regeneration

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Creatine increases IGF‐I and myogenic regulatory factor mRNA in C₂C₁₂ cells