Creatine kinase 2 mass measurement: methods comparison and study of the matrix effect

Sánchez, Mariana López; Canalías, Francesca; Palencia, Teresa; Gella, F. Javier

doi:10.1016/s0009-8981(99)00149-7

Cited by 12 publications

(8 citation statements)

References 11 publications

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“…The activity of CK found in plasma or serum is used to diagnose muscular damage in many species (Hamburg et al, 1991; Hornikova et al, 2009). When CK is found in the blood plasma, it indicates muscle damage (Sáncheza et al, 1999). Rupture of muscles causes the release of CK which is deposited into the blood (Vojtic, 2000).…”

Section: Introductionmentioning

confidence: 99%

The Effects of Pre-slaughter Stress and Season on the Activity of Plasma Creatine Kinase and Mutton Quality from Different Sheep Breeds Slaughtered at a Smallholder Abattoir

Chulayo

Muchenje

2013

Asian Australas. J. Anim. Sci

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The objective of the current study was to determine the effect of pre-slaughter stress, season and breed on the activity of plasma creatine kinase (CK) and the quality of mutton. One hundred and seventy-three (173) castrated sheep from Dormer (DM), South African Mutton Merino (SAMM), Dorper (DP) and Blackhead Persian (BP) sheep breeds were used in the study. The animals were grouped according to age-groups as follows: Group 1 (6 to 8 months), Group 2 (9 to 12 months) and Group 3 (13 to 16 months). Blood samples were collected during exsanguinations using disposable vacutainer tubes for CK analysis. Representative samples of the Muscularis longissimuss thoracis et. lumborum (LTL) were collected from 84 castrated sheep, of different breeds (28 per breed) 24 h after slaughter. The following physico-chemical characteristics of mutton were determined; meat pH (pH24), color (L*, a* and b*), thawing and cooking losses and Warner Braztler Shear Force (WBSF). The activity of plasma CK was significantly higher (p<0.001) in summer (1,026.3±105.06) and lower in winter (723.3±77.75). There were higher values for L* (33.7±0.94), b* (11.5±0.48) and WBSF (29.5±1.46) in summer season than in winter season; L* (29.4±0.64), b* (10.2±0.33) and WBSF (21.2±0.99). The activity of plasma CK was influenced by the type of breed with Dormer having the highest (p>0.001) levels (1,358.6±191.08) of CK. South African Mutton Merino had higher values for pH24 (5.9±0.06), L* (34.2±0.97), b* (12.2±0.50) and WBSF (26.8±1.51) and Blackhead Persian had higher values (35.5±2.17) for cooking loss (CL%) than the other breeds. Computed Principal Component Analyses (PCA) on the activity of plasma CK and physico-chemical characteristics of mutton revealed no correlations between these variables. However, positive correlations were observed between pH24, L*, a*, b*, CL% and WBSF. Relationships between pre-slaughter stress, CK activity and physico-chemical characteristics of mutton were also observed. It was therefore concluded that although mutton quality and creatine kinase were not related, pre-slaughter stress, season and breed affected the activity of creatine kinase and mutton quality.

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Section: Introductionmentioning

confidence: 99%

The Effects of Pre-slaughter Stress and Season on the Activity of Plasma Creatine Kinase and Mutton Quality from Different Sheep Breeds Slaughtered at a Smallholder Abattoir

Chulayo

Muchenje

2013

Asian Australas. J. Anim. Sci

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“…One cannot be sure that the physical or chemical state of the cTnI in the HyTest preparation, e.g., its solubility, is the same as that for cTnI in vivo. The purification procedure can lead to partial alteration of cTnI structure with the consequence that the probability of significant effects by the complex matrix becomes high, which might affect the immunoassay reaction (3,4 As in other studies (6 ), we have also seen problems in the stability of the material. After thawing, we noticed a significant loss in the specific activity within a few hours.…”

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confidence: 52%

“…Activated protein C (APC) inactivates factor Va and requires factor V as a cofactor in the APC-mediated inactivation of factor VIII (1,2 ). Resistance to APC action leads to increased risk of thrombosis and is mostly attributable to a single point mutation in the factor V gene (factor V Leiden) with a G-to-A substitution at nucleotide 1691 (3 ).…”

Section: To the Editormentioning

confidence: 99%

The Interfering Component in Cardiac Troponin I Immunoassays: Need for Further Experimental Evidence

Panteghini

2004

Clinical Chemistry

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“…Previous results (6)(7)(8) showed that commercial immunoassays are affected by the matrix of the analyzed material and also that many materials, including BCR 608, are not commutable. Previous results (6)(7)(8) showed that commercial immunoassays are affected by the matrix of the analyzed material and also that many materials, including BCR 608, are not commutable.…”

Section: Discussionmentioning

confidence: 99%

Certification of the Mass Concentration of Creatine Kinase Isoenzyme 2 (CK-MB) in the Reference Material BCR 608

Sánchez

Gella

Profilis

et al. 2001

Clinical Chemistry and Laboratory Medicine

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We describe the certification of a mass concentration value in the already prepared creatine kinase-2 reference material (BCR 608). Creatine kinase-2 was purified from human heart. The purified enzyme was diluted in order to measure its protein concentration by the Doetsch method. A protein concentration value of 124.30+/-13.17 mg/l was assigned to the stock solution of purified creatine kinase-2. This stock solution was diluted in 25 mmol/l piperazine-N,N'-bis[2-ethanesulfonic acid] (PIPES) pH 7.2, containing 2 mmol/l ADP, 5 mmol/l 2-mercaptoethanol, 154 mmol/l sodium chloride and 50 g/l human albumin to obtain a stable liquid standard of known creatine kinase-2 mass concentration (80.36 microg/l). This standard was then used to recalculate the creatine kinase-2 mass concentration measured in the BCR 608 material by immunoassay. The mass concentration of creatine kinase-2 in samples of reconstituted BCR 608 was certified to be 93.30+/-9.65 microg/l.

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Creatine kinase 2 mass measurement: methods comparison and study of the matrix effect

Cited by 12 publications

References 11 publications

The Effects of Pre-slaughter Stress and Season on the Activity of Plasma Creatine Kinase and Mutton Quality from Different Sheep Breeds Slaughtered at a Smallholder Abattoir

The Effects of Pre-slaughter Stress and Season on the Activity of Plasma Creatine Kinase and Mutton Quality from Different Sheep Breeds Slaughtered at a Smallholder Abattoir

The Interfering Component in Cardiac Troponin I Immunoassays: Need for Further Experimental Evidence

Certification of the Mass Concentration of Creatine Kinase Isoenzyme 2 (CK-MB) in the Reference Material BCR 608

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