Creatine kinase in developing skeletal and cardiac muscle of the rat

Ziter, Fred A.

doi:10.1016/0014-4886(74)90193-9

Cited by 52 publications

(27 citation statements)

References 12 publications

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“…In the present study, MM-CK was undetectable in most animals at about a week after muscle mincing, indicating that all original myofibers had been destroyed and newly formed myotubes had not yet made significant amounts of MM-CK. Subsequent isozyme changes during regeneration resemble those found in development (Ziter, 1974;Lough and Bischoff, 19771, with a n early increase in BB-CK followed by a peak of MB-CK activity at about 1.5 weeks and a predominance of MM-CK at about 3 weeks. Although the cellular location of isozymes was not studied here, both the M-and B-CK subunits are expressed in developing skeletal muscle (Perriard, 1979).…”

Section: Normal Mincesupporting

confidence: 57%

“…After adding glycerol to 10% and bromphenol blue to 0.002%, the samples were loaded over 5 x 70 mm tube gels containing a 3% stacking gel and 5% running gel. Each electrophoresis run also contained, as a control, a sample of neonatal rat heart homogenate, which includes all three isozymes (Ziter, 1974). Gels were mounted in a GE 2/4 unit (Pharmacia, Piscataway, NJ) modified to contain a cooling coil in both the upper and lower buffer compartments.…”

Section: Creatine Kinase Assaymentioning

confidence: 99%

“…The M subunit is the predominant form expressed in mature skeletal myofibers (fast-twitch myofibers contain greater than 90% MM-CK (Yamashita and Yoshioka, 1991)), but the B subunit is also expressed during myogenesis (Lough and Bischoff, 1977). Developing muscle exhibits a characteristic pattern of changes among the MM, MB, and BB isozymes (Ziter, 1974), thought to be related to the changing energy metabolism of the developing tissue (Perriard et al, 1989). Because expression of the M subunit is linked to myogenic differentiation factors (Lassar et al, 1989;Yutzey et al, 1990) and MM-CK activity is closely correlated with the degree of myotube formation and maturation (Lough and Bischoff, 1977;Perriard, 1979), the enzyme is a n excellent index of skeletal muscle differentiation (Morris, 1978) during regeneration.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of skeletal muscle regeneration

Bischoff

Heintz

1994

Developmental Dynamics

161

103

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We have studied the effect of adding extra satellite cells or soluble factors from crushed muscle on regeneration of minced fragments from rat tibialis muscle. The muscle mince was wrapped in an artificial epimysium to prevent adhesions and cell immigration from adjacent muscles. Regeneration was quantitatively assessed by electrophoretic determination of the muscle-specific form of creatine kinase. Control minces exhibited three periods of change in creatine kinase activity during a 7-week regeneration period. Activity fell rapidly during the first week, then rose gradually from 1 3 weeks and increased more rapidly from 3-7 weeks. To augment the original complement of myogenic cells, satellite cells were isolated from the contralateral muscle, purified by density gradient centrifugation, and expanded in culture for 3 days before adding to the muscle mince. The added cells resulted in a 3-fold enhancement of creatine kinase activity throughout the regeneration period. Soluble muscle extract incorporated into a collagen matrix also stimulated regeneration when added to muscle mince. The extract accelerated the rate of creatine kinase increase during the 1 3 week period beyond that observed in the control or cell augmented mince, suggesting that factors in the extract may facilitate revascularization or reinnervation. The specific activity of creatine kinase was increased in regenerates augmented with both cells and extract, indicating that the effects enhance primarily myogenic processes.

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Section: Normal Mincesupporting

confidence: 57%

Section: Creatine Kinase Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhancement of skeletal muscle regeneration

Bischoff

Heintz

1994

Developmental Dynamics

161

103

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“…We also tested cell extracts from day 11 DMSO-treated cultures for the presence of the muscle-specific isozyme of CPK, CPK-M (1,25,30). Only a small amount (<5%) of the CPK activity was eluted off DEAE-Sephadex columns at salt concentrations indicative of the CPK-MB heterodimer.…”

Section: Methodsmentioning

confidence: 99%

“…Only a small amount (<5%) of the CPK activity was eluted off DEAE-Sephadex columns at salt concentrations indicative of the CPK-MB heterodimer. All other activity was of the BB form, the form present in nonmuscle tissue and in embryonic muscle cells (1,25,30).…”

Section: Methodsmentioning

confidence: 99%

Induced muscle differentiation in an embryonal carcinoma cell line.

Edwards¹,

Harris²,

McBurney³

1983

Mol. Cell. Biol.

174

107

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Cells of the teratocarcinoma-derived line P19S1801A1 (01A1) are pluripotent embryonal carcinoma cells and can be induced to differentiate when aggregated and exposed to dimethyl sulfoxide. Many nonneural cell types appear in dimethyl sulfoxide-treated cultures, cardiac and skeletal muscle being the most easily identified. We have used immunofluorescence procedures with monoclonal antibodies directed against muscle myosin to confirm and quantitate the number of muscle cells formed. A monoclonal antibody reactive with an embryonal carcinoma-specific surface antigen was used to confirm the disappearance of undifferentiated cells after dimethyl sulfoxide treatment. Cardiac muscle cells developed within 4 to 5 days of drug exposure, but skeletal muscle cells did not become evident until 7 to 8 days. We have isolated a mutant cell line (D3) which appears to be incapable of muscle development but which does form neurons and glial cells when exposed to high retinoic acid concentrations. We propose that this system will be useful for investigation of the means by which pluripotent cells become committed to development along the striated muscle lineages.

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Spatial distribution of “tissue‐specific” antigens in the developing human heart and skeletal muscle. I. An immunohistochemical analysis of creatine kinase isoenzyme expression patterns

Wessels

Vermeulen

Virágh³

et al. 1990

Anat. Rec.

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Using monoclonal antibodies against the M and B subunit isoforms of creatine kinase (CK) we have investigated their distribution in developing human skeletal and cardiac muscle immunohistochemically. It is demonstrated that in skeletal muscle, a switch from CK-B to CK-M takes place around the week 8 of development, whereas in the developing heart, CK-M is the predominant isoform from the earliest stage examined onward (i.e., 4 1/2 weeks of development). In all hearts examined, local differences in concentration of the CK isoforms are observed. The CK-M expression in the developing outflow tract (OFT) and conduction system is described in detail. Between the weeks 5 and 7 of development, the distal portion of the OFT is characterized by low CK-M expression, whereas around the week 8-10 of development the myocardium around the developing semilunar valves in the OFT expresses a very high level of CK-M. At all stages examined, a relatively low CK-M level is observed in those regions in which the "slow" components of the conduction system do develop (e.g., the sinoatrial junction and atrioventricular junction), whereas a relatively high concentration of CK-M is observed in those areas that are destined to become the "fast" components, i.e., the subendocardial myocardium of the ventricles. The high expression of CK-M in the developing "fast components" of the conduction system contrasts with the relatively low expression of CK-M in the force-producing myocardium of the interventricular septum and free ventricular wall.

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Creatine kinase in developing skeletal and cardiac muscle of the rat

Cited by 52 publications

References 12 publications

Enhancement of skeletal muscle regeneration

Enhancement of skeletal muscle regeneration

Induced muscle differentiation in an embryonal carcinoma cell line.

Spatial distribution of “tissue‐specific” antigens in the developing human heart and skeletal muscle. I. An immunohistochemical analysis of creatine kinase isoenzyme expression patterns

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