Creatine Kinase: Structure‐Activity Relationships

Kenyon, George L.; Reed, George H.

doi:10.1002/9780470122990.ch6

Cited by 80 publications

(24 citation statements)

References 166 publications

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“…Such concentrations alone are able to fuel only a brief period of activity before complete ATP depletion. Fortunately, cells are able to draw upon large quantities of intracellular highenergy phosphate from PCr to continuously and efficiently replete ATP through the creatine kinase reaction (Clarke and Sokoloff, 1999;Iyengar, 1984;Kenyon and Reed, 1983;Wallimann et al, 1992;Wyss and Kaddurah-Daouk, 2000).…”

Section: Discussionmentioning

confidence: 99%

Tissue Type-Specific Bioenergetic Abnormalities in Adults with Major Depression

Harper

Jensen

Ravichandran

et al. 2016

Neuropsychopharmacol

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Brain bioenergetic abnormalities have been observed frequently in adults with major depressive disorder (MDD); however, results have been inconsistent regarding whether decreased or increased metabolism was observed. Phosphorus-31 magnetic resonance spectroscopy (31P MRS) allows for the quantification of bioenergetic molecules, containing high-energy phosphates, over the whole brain as well as measuring the differences between gray matter and white matter. We recruited 50 subjects with a current diagnosis of MDD, not currently treated with psychotropic medication, between ages of 18 and 65 (mean ± SD age: 43.4 ± 13.6; 46% female) and 30 healthy volunteers, matched for age and gender (39.0 ± 12.5 years of age; 36.6% female). All subjects received a T1 MP-FLASH scan for tissue segmentation followed by 31P MRS, chemical shift imaging scan with 84 voxels of data collected over the entire brain utilizing a dual-tuned, protonphosphorus coil to minimize subject movement. Phosphocreatine and inorganic phosphate (Pi) varied in opposite directions across gray matter and white matter when MDD subjects were compared with controls. This finding suggests alterations in high-energy phosphate metabolism and regulation of oxidative phosphorylation in MDD patients. In addition, within the MDD group, gray matter Pi, a regulator of oxidative phosphorylation, correlated positively with severity of depression. These data support a model that includes changes in brain bioenergetic function in subjects with major depression.

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Section: Discussionmentioning

confidence: 99%

Tissue Type-Specific Bioenergetic Abnormalities in Adults with Major Depression

Harper

Jensen

Ravichandran

et al. 2016

Neuropsychopharmacol

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“…Efficiency of energy transfer between the sites of energy production and utilization primarily depends on the creatine kinase (CK) system, which functions in cardiac muscle cells as the main energy buffer keeping a high ATP/ADP ratio and optimal pH simultaneously [1,2,3,4]. It catalyzes a reversible reaction of creatine with ATP in which creatine is phosphorylated [5]. …”

Section: Introductionmentioning

confidence: 99%

Chronic Hypoxia Enhances Expression and Activity of Mitochondrial Creatine Kinase and Hexokinase in the Rat Ventricular Myocardium

Waskova-Arnostova

Kasparova

Elsnicová

et al. 2014

Cell Physiol Biochem

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Background: Creatine kinase (CK) and hexokinase (HK) play a key role in myocardial energy homeostasis. We aimed to determine CK and HK expression and enzyme activity in the left (LV) and right (RV) ventricles of rats adapted for 3 weeks to normobaric hypoxia (10 % O₂) either continuously (CNH) or intermittently with 1-h or 16-h normoxic episode per day. Methods: The Real-Time RT-PCR, Western blot, and enzyme-coupled assays were used. In addition, the effect of CNH on the HK co-localization with mitochondria, which can inhibit apoptosis, was assessed using immunofluorescence techniques. Results: CK and HK activities increased in the LV during all hypoxic adaptations, which was consistent with elevated protein levels of mitochondrial mtCKs, cytosolic CKB, HK1, and HK2 isoforms. Enzyme activities also increased in the hypoxic RV, but only CKB protein was elevated. No effect of CNH on HK1 or HK2 co-localization with mitochondria was observed. Conclusion: Up-regulation of mtCKs and HK isoforms may stimulate the respiratory chain and help to maintain energy homeostasis of chronically hypoxic myocardium and prevent oxidative stress. In this way, CK and HK enzymes can possibly participate in the establishment of ischemia-resistant phenotype of chronically hypoxic hearts.

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“…) and by shuttling the energy between different cellular compartments (Wallimann et al, 1992). Arginine kinase (AK), as a member of the phosphagen kinases in invertebrates (Kenyon and Reed, 1983;Strong and Ellington, 1993), is highly expressed in energetically demanding reactions such as the formation of the siliceous skeleton, which has been demonstrated in Suberites domuncula (PerovicOttstadt et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

ATP distribution and localization of mitochondria in Suberites domuncula (Olivi 1792) tissue

Lukić-Bilela

Perović-Ottstadt

Walenta

et al. 2011

Journal of Experimental Biology

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SUMMARYThe metabolic energy state of sponge tissue in vivo is largely unknown. Quantitative bioluminescence-based imaging was used to analyze the ATP distribution of Suberites domuncula (Olivi 1792) tissue, in relation to differences between the cortex and the medulla. This method provides a quantitative picture of the ATP distribution closely reflecting the in vivo situation. The obtained data suggest that the highest ATP content occurs around channels in the sponge medulla. HPLC reverse-phase C-18, used for measurement of ATP content, established a value of 1.62mol ATPg -1 dry mass in sponge medulla, as opposed to 0.04mol ATPg -1 dry mass in the cortex, thus indicating a specific and defined energy distribution. These results correlate with the mitochondria localization, determined using primary antibodies against cytochrome oxidase c subunit 1 (COX1) (immunostaining), as well as with the distribution of arginine kinase (AK), essential for cellular energy metabolism (in situ hybridization with AK from S. domuncula; SDAK), in sponge sections. The highest energy consumption seemed to occur in choanocytes, the cells that drive the water through the channel system of the sponge body. Taken together, these results showed that the majority of energetic metabolism in S. domuncula occurs in the medulla, in the proximity of aqueous channels.

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Creatine Kinase: Structure‐Activity Relationships

Cited by 80 publications

References 166 publications

Tissue Type-Specific Bioenergetic Abnormalities in Adults with Major Depression

Tissue Type-Specific Bioenergetic Abnormalities in Adults with Major Depression

Chronic Hypoxia Enhances Expression and Activity of Mitochondrial Creatine Kinase and Hexokinase in the Rat Ventricular Myocardium

ATP distribution and localization of mitochondria in Suberites domuncula (Olivi 1792) tissue

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