Creatine Phosphokinase Isoenzymes in the Diagnosis and Assessment of Myocardial Injury

Natural history and evaluation of Q waves during acute myocardial infarction.

“…Total plasma CK activity was measured spectrophotometrically (using Cecil 272 system spectrophotometer, Cecil Instruments, Cambridge) by the method of Oliver (1955) as modified by Hearse et al (1973). Using this method the upper limit of plasma CK for healthy subjects is 50 mU/ml (Ogunro et al, 1976). Samples with activity more than 250 mU/m1 were diluted before repeat determination of total CK.…”

“…In order to minimise the dilution-activation effect observed with plasma CK, heat inactivated plasma was employed as diluent (Graig et al, 1967). Isoenzymes were separated by electrophoresis on agarose gels and quantified in aqueous solution by fluorimetry (Ogunro et al, 1976). MB CK isoenzyme release curves were constructed by measuring the plasma MB CK activity (mU/ml) at the stated time intervals after the onset of chest pain.…”