Creatine supplementation reduces skeletal muscle degeneration and enhances mitochondrial function in mdx mice

Passaquin, Anne-Catherine; Renard, Mathilde; Kay, Laurence; Challet, Corinne; Mokhtarian, Armand; Wallimann, Theo; Rüegg, Urs T.

doi:10.1016/s0960-8966(01)00273-5

Cited by 120 publications

(87 citation statements)

References 40 publications

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“…Similar to previous studies, 15,19 we elected to begin therapy in the mice at 4 weeks of age, thus encompassing the substantial muscle degeneration and regeneration that occur at 3-5 weeks of age. 14 This differs from the study by Passaquin et al, 36 who began feeding CrM to pregnant mdx mothers.…”

Section: Discussionmentioning

confidence: 86%

“…46 Several studies have demonstrated the benefit from creatine monohydrate supplementation in mdx mice 15,26,36 and in patients with DMD. 25,47,52 Although increasing muscle creatine levels may be the mechanism behind a therapeutic effect, some studies have not measured muscle phosphocreatine and total creatine levels.…”

Section: Discussionmentioning

confidence: 99%

“…25,47,52 Although increasing muscle creatine levels may be the mechanism behind a therapeutic effect, some studies have not measured muscle phosphocreatine and total creatine levels. 15,26,36 Among those that have, 26,36 only one study found significant uptake into mdx skeletal muscle (extensor digitorum longus muscle). 26 In our study, although not significant, the COMB and COMB ϩ PRED groups had the greatest increases to total creatine levels.…”

Section: Discussionmentioning

confidence: 99%

“…However, boys with DMD do not typically present until their preschool years, at which point muscle remodeling has already begun. Consequently, the magnitude of benefit observed from therapies in the mdx model 36,37 might not readily translate to boys with DMD. It is important to note that CrM, which was not robustly beneficial in the current study, has already demonstrated efficacy in boys with DMD when given well after the onset of weakness, 47,52 as well as in mdx mice aged 100 -110 days.…”

Section: Discussionmentioning

confidence: 99%

“…37 CrM-supplemented mdx mice demonstrated reduced necrosis in type II muscle fibers and an enhanced skeletal muscle oxidative capacity. 36 CrM supplementation also increases measures of strength and reduce a marker of bone breakdown in boys with DMD. 25,47 Conjugated linoleic acid (CLA) represents a number of different isomers of the essential fatty acid linoleic acid containing conjugated double bonds.…”

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confidence: 98%

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Nutritional therapy improves function and complements corticosteroid intervention in mdx mice

et al. 2005

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Corticosteroid therapy for Duchenne muscular dystrophy is effective but associated with long-term side effects. To determine the potential therapeutic benefit from four nutritional compounds (creatine monohydrate, conjugated linoleic acid, ␣-lipoic acid, and ␤-hydroxy-␤-methylbutyrate) alone, in combination, and with corticosteroids (prednisolone), we evaluated the effects on several variables in exercising mdx mice. Outcome measures included grip strength, rotarod performance, serum creatine kinase levels, muscle metabolites, internalized myonuclei, and retroperitoneal fat pad weight. In isolation, each nutritional treatment showed some benefit, with the combination therapy showing the most consistent benefits. Prednisolone and the combination therapy together provided the most consistent evidence of efficacy; increased peak grip strength (P Ͻ 0.05), decreased grip strength fatigue (P Ͻ 0.05), decreased number of internalized myonuclei (P Ͻ 0.01), and smaller retroperitoneal fat pad stores (P Ͻ 0.001). This study provided evidence for therapeutic benefit from a four-compound combination therapy alone, and in conjunction with corticosteroids in the mdx model of DMD.

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Section: Discussionmentioning

confidence: 86%