Creating biotransformation of volatile fatty acids and octanoate as co-substrate to high yield medium-chain-length polyhydroxyalkanoate

Li, Dongna; Ma, Xiaojun; Yin, Fen; Qiu, Yujuan; Yan, Xu

doi:10.1016/j.biortech.2021.125031

Cited by 13 publications

(8 citation statements)

References 39 publications

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“…This is considerably higher than the 34 wt% reported in previous research investigating octanoate‐fed enrichments, although the mcl‐PHA fraction was similar (i.e., HHx fraction of 0.08 and HO fraction of 0.62) (Chen et al, 2018). Li et al (2021) also obtained a lower PHA content (53 wt%) when enriching with octanoate as a cosubstrate, which also resulted in a significantly lower mcl‐PHA fraction (i.e., HHx fraction of 0.08 and HO fraction of 0.26).…”

Section: Discussionmentioning

confidence: 99%

Production of medium‐chain‐length PHA in octanoate‐fed enrichments dominated by Sphaerotilus sp.

Vermeer

Depaz

Berg

et al. 2022

Biotech & Bioengineering

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Medium-chain-length polyhydroxyalkanoate (mcl-PHA) production by using microbial enrichments is a promising but largely unexplored approach to obtain elastomeric biomaterials from secondary resources. In this study, several enrichment strategies were tested to select a community with a high mcl-PHA storage capacity when feeding octanoate. On the basis of analysis of the metabolic pathways, the hypothesis was formulated that mcl-PHA production is more favorable under oxygen-limited conditions than short-chain-length PHA (scl-PHA). This hypothesis was confirmed by bioreactor experiments showing that oxygen limitation during the PHA accumulation experiments resulted in a higher fraction of mcl-PHA over scl-PHA (i.e., a PHA content

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Section: Discussionmentioning

confidence: 99%

Production of medium‐chain‐length PHA in octanoate‐fed enrichments dominated by Sphaerotilus sp.

Vermeer

Depaz

Berg

et al. 2022

Biotech & Bioengineering

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“…Fivecarbon sugars can also be utilized through the pentose phosphate pathway, however, there is only few microbes that could e ciently convert such sugars into PHA due to the fact that most PHA-producing strains which preferred 6-carbon sugars has low 5-carbon sugar metabolic activities (Li et al, 2021). In a rare case, Burkholderia sacchari DSM 17165 was reported to be able to effectively produce PHA with xylose and arabinose as carbon sources (Li et al, 2021). Therefore, in the present investigation, 20 gL − 1 of six-carbon sugars, including glucose, mannitose, lactose, starch, or sucrose were employed as carbon source in ZMA medium with cultivation pH and temperature of pH 7 and 35 o C.…”

Section: Carbon Sourcesmentioning

confidence: 99%

“…Lactose was also reported to be utilized in the production of both PHA and biohydrogen by a two-step fermentation of deproteinized cheese whey, in which lactose is one of the major constituents (Colombo et al, 2019). Fivecarbon sugars can also be utilized through the pentose phosphate pathway, however, there is only few microbes that could e ciently convert such sugars into PHA due to the fact that most PHA-producing strains which preferred 6-carbon sugars has low 5-carbon sugar metabolic activities (Li et al, 2021). In a rare case, Burkholderia sacchari DSM 17165 was reported to be able to effectively produce PHA with xylose and arabinose as carbon sources (Li et al, 2021).…”

Section: Carbon Sourcesmentioning

confidence: 99%

Evaluation of Polyhydroxyalkanoate (PHA) Synthesis by Pichia Sp. TSLS24 Yeast Isolated in Vietnam

Thu¹,

Hoang²,

Cuong³

et al. 2022

Preprint

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Nowadays, polyhydroxyalkanoates (PHAs) is of great interest by scientists and biodegradable polymer market due to their outstanding properties such as high biodegradability in various conditions and processing flexibility. Many PHAs-synthesizing microorganisms, including normal and halophilic bacteria, and algae, were investigated. However, to the best of our knowledge, there is still little studies on PHAs-producing marine yeast. In the present study, a halophilic yeast strain isolated from Spratly Island in Vietnam were investigated for synthesis of PHA by growing the yeast in a specific medium containing Nile red dye. The strain was identified by 26S rDNA analysis as Pichia kudriavzevii TSLS24 and registered at Genbank database under code OL757724. The amount of PHA synthesized was quantified by measuring the intracellular materials (predicted as PHA) by gravimetric method, subsequently confirmed by Fourier transform infrared spectroscopic and nuclear magnetic resonance spectroscopic analyses. Under optimal growth conditions of 35 oC and pH 7 with supplementation of glucose and yeast extract at 20 and 10 g/L, the strain isolated achieved PHA content and concentration of 43.4% and 1.8 g/L. Degradation rate of PHA produced was 28% after 28 days of incubation in sea water.

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“…The market size (kilotons/year) and market price (€/ton) of acetate, propionate, and butyrate were 14,000–17,000 and 400–800, 350–470 and 2000–2500, and 90–105 and 1500–1650, respectively, and their total global market demand in 2020 was 18,500 kilotons . Recently, studies on VFA bioproduction from organic waste have aroused extensive interest as the waste-derived VFAs can be utilized as the substrate to produce many valorized products, such as sustainable aviation fuel, medium-chain fatty acids, and environmentally biodegradable polymers polyhydroxyalkanoates, or served as the carbon source to enhance biological municipal wastewater nutrient removal performance. − It is of great significance to effectively improve the biological VFA production from organic wastes.…”

Section: Introductionmentioning

confidence: 99%

Amino Acid Configuration Affects Volatile Fatty Acid Production during Proteinaceous Waste Valorization: Chemotaxis, Quorum Sensing, and Metabolism

Wang

Zhang

Huang

et al. 2022

Environ. Sci. Technol.

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During proteinaceous waste valorization to produce volatile fatty acids (VFAs), protein needs to be hydrolyzed to amino acids (AAs), but the effects of the configuration of AAs on their biotransformation and VFA production have not been investigated. In this study, more residual d-AAs than their corresponding l-AAs were observed after VFAs were produced from kitchen waste in a pilot-scale bioreactor. For all AAs investigated, the VFA production from d-AAs was lower than that from corresponding l-AAs. The metagenomics and metaproteomics analyses revealed that the l-AA fermentation system exhibited greater bacterial chemotaxis and quorum sensing (QS) than d-AAs, which benefited the establishment of functional microorganisms (such as Clostridium, Sedimentibacter, and Peptoclostridium) and expression of functional proteins (e.g., substrate transportation cofactors, l-AA dehydrogenase, and acidogenic proteins). In addition, d-AAs need to be racemized to l-AAs before being metabolized, and the difference of VFA production between d-AAs and l-AAs decreased with the increase of racemization activity. The findings of the AA configuration affecting bacterial chemotaxis and QS, which altered microorganism communities and functional protein expression, provided a new insight into the reasons for higher l-AA metabolism than d-AAs and more d-AAs left during VFA production from proteinaceous wastes.

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Creating biotransformation of volatile fatty acids and octanoate as co-substrate to high yield medium-chain-length polyhydroxyalkanoate

Cited by 13 publications

References 39 publications

Production of medium‐chain‐length PHA in octanoate‐fed enrichments dominated by Sphaerotilus sp.

Production of medium‐chain‐length PHA in octanoate‐fed enrichments dominated by Sphaerotilus sp.

Evaluation of Polyhydroxyalkanoate (PHA) Synthesis by Pichia Sp. TSLS24 Yeast Isolated in Vietnam

Amino Acid Configuration Affects Volatile Fatty Acid Production during Proteinaceous Waste Valorization: Chemotaxis, Quorum Sensing, and Metabolism

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