Creating Efficient and Specific Peroxidase‐Like Atomic Cu‐N‐C Centers via Axial Strong‐Metal‐Support‐Interaction for Enzymatic Biodetection

Abstract

Exploring rapid, sensitive, and multifunctional biosensors is of great significance in medical diagnosis and biotechnology, while creating Cu‐N‐C center‐based enzymatic biodetection system is still a great challenge for colorimetric biosensing fields. Herein, inspired by the axial coordination structure of the natural enzyme, an efficient and specific Cu‐N‐C center‐based peroxidase‐like biocatalyst is created via axial strong‐metal‐support‐interaction (SMSI) between WOx support and Cu‐Nx‐based carbon shell, denoted as Cu‐NC‐WO3. This analysis discloses that the SMSI effect induces electron transfer from Cu to WOx, thus making the valence state of Cu higher and consequently suppressing the O intermediates' adsorption. Owing to this unique structure, the Cu‐NC‐WO3 exhibits exceptional peroxidase (POD) like activities (Km = 3.57 × 10−3 m, Vmax = 13.80 × 10−6 m s−1, and turnover number (TON) = 206.13 × 10−3 s−1 for H2O2), which is more efficient than the other Cu‐Nx center‐based biocatalysts and also many recently reported peroxidase‐like nanomaterials. Meanwhile, this Cu‐NC‐WO3 shows substrate selectivity, low detection limit, and high resistance to extreme environments for abundant H2O2‐related biomarkers, which are revealed by colorimetric studies. Thus, as a helpful method for accurate biodetection, it is believed this work promises to offer rapid, specific, and inexpensive colorimetric biosensors for regions with bare medical resources, as well as new strategies for the structural engineering of enzyme‐like biocatalysts.

Facile Fabrication of Wood-Derived Porous Fe3C/Nitrogen-Doped Carbon Membrane for Colorimetric Sensing of Ascorbic Acid

“…Once the catalyst, H 2 O 2 , and TMB were all combined in the system, the blue color emerged (curve and inset A1 in Figure 3a). This proved that the Fe 3 C/N-C could decompose H 2 O 2 , which is responsible for triggering the oxidation reaction of TMB into ox-TMB that showed up as an absorption peak at 652 nm in the UV-Vis spectrum [66,67].…”

“…Once the catalyst, H2O2, and TMB were all combined in the system, the blue color emerged (curve and inset A1 in Figure 3a). This proved that the Fe3C/N-C could decompose H2O2, which is responsible for triggering the oxidation reaction of TMB into ox-TMB that showed up as an absorption peak at 652 nm in the UV-Vis spectrum [66,67]. Several parameters, such as reaction time, pH, temperature, and the concentration of H2O2 and TMB, could affect the catalytic performance of Fe3C/N-C [11].…”