Creatinine biosensors: principles and designs

Killard, Anthony J.; Smyth, Malcolm R.

doi:10.1016/s0167-7799(00)01491-8

Cited by 142 publications

(64 citation statements)

References 21 publications

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“…However, there are other interesting molecules to detect for clinical interest. Over the last two decades there have been proposed biosensors for lactate [31], cholesterol [43], glutamate [38], creatinine [21], etc. Biomarkers are another large family of biomolecules arising interest, since they are able to point out if a biological process, a disease, or a response to a therapeutic intervention is in progress.…”

Section: Targetsmentioning

confidence: 99%

Integrated biosensors for personalized medicine

Micheli

Boero

Baj-Rossi

et al. 2012

Proceedings of the 49th Annual Design Automation Conference

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Biosensors are heterogenous devices, incorporating biological structures combined with electronics, optical or other readout systems. They have been developed for detecting different biomolecules and/or pathogens and represent a key technology for advanced and pointof-care diagnostics as well as patient monitoring. In this paper we present a systematic classification of biosensors described in literature, particularly focusing on nanotechnology-based sensing. Then, we present our approach to develop electrochemical biosensors for measuring metabolites and anticancer drugs, based on a platform for multiple target detection. This platform is modular and achieves a clear separation between the chemical and the electrical components, thus easing design and manufacturing. It shows superior performance thanks to the excellent properties of electron transfer and selectivity showed by enzymes immobilized on carbon nanotubes.

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Section: Targetsmentioning

confidence: 99%

Integrated biosensors for personalized medicine

Micheli

Boero

Baj-Rossi

et al. 2012

Proceedings of the 49th Annual Design Automation Conference

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“…8 Of the many creatinine sensors subsequently reported, 9 the most common technology has been an amperometric threeenzyme system consisting of immobilized creatinine amidohydrolase, creatine amidohydrolase, and sarcosine oxidase, which sequentially converts creatinine to creatine, sarcosine, and thence to hydrogen peroxide, glycine, and formaldehyde:…”

Section: Monitoring Renal Function: Urea and Creatininementioning

confidence: 99%

“…Potentiometric creatinine biosensors are generally based on the immobilization of creatinine iminohydrolase (deiminase) at the surface of an NH 4 + -sensitive ion-selective electrode: 9,10 Creatinine…”

Section: Monitoring Renal Function: Urea and Creatininementioning

confidence: 99%

Biosensors for Monitoring Metabolites in Clinical Medicine

Pickup

2007

Handbook of Biosensors and Biochips

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Biosensors for small‐molecular‐weight metabolites are finding increasing use in medicine for rapid, reagentless point‐of‐care measurement and continuous in vivo monitoring. Though glucose is the most well‐established clinical biosensor application, others include urea and creatinine for assessing renal function; lactic acid for monitoring tissue oxygenation; uric acid for assessing nucleic acid metabolism; cholesterol, triglyceride, and ketones for monitoring lipid metabolism; and bilirubin for liver and blood disorders. Many metabolite biosensors are enzyme electrodes, based on immobilized oxidases, hydrolases, or other enzymes, with amperometric or potentiometric electrochemical reaction monitoring, but other bioreceptors such as computer‐designed proteins and other reaction detection methods such as fluorescence are in use.

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“…7,8 To enhance the specificity, enzymatic methods are often used. [9][10][11][12] The most popular among them is the three-enzyme method, in which creatinine amidohydrolase (CA), creatine amidinohydrolase (CI), and sarcosine oxidase (SO) can be employed to catalyze the hydrolysis of creatinine to hydrogen peroxide (H 2 O 2 ), which can then be detected amperometrically. 9 Besides, creatinine iminohydrolase (CIH) was utilized to catalyze the hydrolysis of creatinine to ammonia (NH 3 ); the NH 3 was then detected by potentiometry.…”

Section: Introductionmentioning

confidence: 99%

A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction

Fernandes¹,

Souza²,

Oliveira³

et al. 2017

J. Braz. Chem. Soc.

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A method based on disposable pipette extraction (DPX) was successfully applied to creatinine determination in urine samples analysis using liquid chromatography with ultraviolet spectrophotometric detection (DPX/LC-UV). DPX variables, number of draw/eject cycles, sample pH, and type of the desorption solvent, were employed in a factorial experimental design to optimize the sorption equilibrium and time analysis. Among the evaluated DPX variables, the highest extraction efficiency was obtained with 500 µL of urine sample mixed with 1 mL of borate solution (pH 9) with one draw/eject cycle followed by liquid desorption of 1 mL of methanol in seven draw/eject cycles. The developed DPX/LC-UV method showed a linear response from the limit of quantification of 0.317 to 3.390 g L -1 with r 2 = 0.996 and inter-day precision with a coefficient of variation below 8.8%. Based on these results, the proposed method can be a useful tool for determining the creatinine levels in urine samples.

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Creatinine biosensors: principles and designs

Cited by 142 publications

References 21 publications

Integrated biosensors for personalized medicine

Integrated biosensors for personalized medicine

Biosensors for Monitoring Metabolites in Clinical Medicine

A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction

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