Creatinine-Iron Complex and Its Use in Electrochemical Measurement of Urine Creatinine

Kumar, Vanita; Hebbar, Suraj; Kalam, Rahila; Panwar, Sachin; Prasad, Sujay; Srikanta, S.; Krishnaswamy, P. R.; Bhat, Navakanta

doi:10.1109/jsen.2017.2777913

Cited by 38 publications

(27 citation statements)

References 26 publications

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“…Thereby, an increase in absorbance intensity was observed in relation to the increase in creatinine concentration at a wavelength of 333 nm and a shoulder appear at wavelength range from 450 to 535 nm suggesting thus a complex formation as described elsewhere . The presence of several donor groups in the tautomeric form of creatinine determines its strong ability to form complexes with several metals, such as copper, platinum, and zinc , and in this case, the formation of a yellow complex between the Fe 3+ ion and creatinine is evident.…”

Section: Resultsmentioning

confidence: 99%

New Disposable Electrochemical Paper‐based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample

et al. 2020

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A disposable electrochemical paper‐based analytical device was constructed based on use of sequential analysis with multiplexed working electrodes and applied for the determination of glucose, creatinine, and uric acid. The device was constructed with 16 microfluidic channels, with 16 working electrodes arranged in four set with four components surrounding the sample injection hole. In addition, a commercial multiplexing module was used, which allowed for multiplexing of the 16 working electrodes. This design allowed for radial and homogeneous sample elution to each sensing spot for high throughput analysis. In the multiplexed determinations, distinct electrochemical procedures were employed for each analyte. Furthermore, each working electrode spot was modified to increase the respective analytical signals. For glucose detection, the sensor was based on electron mediation by ferrocenecarboxylic acid over the modified surface with glucose oxidase. The principle for creatinine detection was based on electrochemical reduction of non‐complexed Fe3+ in excess after complex formation between Fe3+ and creatinine in the chemical step. The anodic peak current responses for uric acid detection increased due to working electrode surface modification with carbon black nanoparticles. In the multiplexed analysis, the device provided limits of detection of 0.120 mmol L−1, 0.084 mmol L−1, and 0.012 mmol L−1 for glucose, creatinine, and uric acid, respectively. The developed device was successfully applied in the analyses of real urine samples.

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Section: Resultsmentioning

confidence: 99%

New Disposable Electrochemical Paper‐based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample

et al. 2020

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“…The PoC device technology was developed at the Indian Institute of Science, Bangalore, over the last 5 years, and it is based on nonenzymatic- and nonantibody-based electrochemical biosensing technology. 10 , 11 , 12 , 13 , 14 The PoC measurement is done on electrochemical disposable test strips that contain a membrane impregnated with patented sensing chemistries. This device, unlike many other devices, performs quantitative measurement of the analytes in question, at any remote area, with absolutely no infrastructure requirements.…”

Section: Methodsmentioning

confidence: 99%

“…Our published references can be further referred for any of the fine details of the technology, method, and sensing chemistries for hemoglobin, HbA 1c , serum albumin, urine albumin, and ACR. 10 , 11 , 12 , 13 , 14 …”

Section: Methodsmentioning

confidence: 99%

Application of a Nanotechnology-Based, Point-of-Care Diagnostic Device in Diabetic Kidney Disease

Kumar¹,

Hebbar²,

Bhat³

et al. 2018

Kidney International Reports

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IntroductionEarly detection of diabetes mellitus (DM) and diabetic kidney disease (DKD) is important for preventing end-stage renal failure and reducing cardiovascular complications. Availability of a validated point-of-care (PoC) device that can measure various DKD markers would be useful in this respect, especially in resource-poor parts of the world.MethodsWe validated a novel nanotechnology-based multianalyte PoC device (minimally invasive and does not require trained medical personnel) against laboratory gold standard tests for the detection of 5 biomarkers related to management of DM and DKD. The prospective study was funded by an International Society of Nephrology American Nephrologists of Indian Origin grant in 2 phases: (i) proof of concept: random samples were tested for the analytes with the PoC device and correlated with the laboratory gold standard; and (ii) clinical validation in a well-characterized cohort of patients. A nonenzymatic- and nonantibody-based electrochemical PoC device for quantitative measurement of markers—glycosylated hemoglobin (HbA1c), hemoglobin, serum albumin, microalbuminuria, urine creatinine, and albumin-to-creatinine ratio−was developed and used in this study. The disposable strips were interfaced with a multipotentiostat hand-held PoC device (3.7-V rechargeable lithium battery, 5-inch touch screen, Bluetooth enabled) working in amperometry mode, which provided the results in <1 minute. Data were analyzed using linearity plots and Bland-Altman difference plot analysis.ResultsA total of 4717 individuals were screened during the study (phase 1: 2576 and phase 2: 2141.) In phase 2, samples were tested in 529 subjects (346 females)—120 subjects with type 1 DM, 255 subjects with type 2 DM, 54 subjects without DM, 400 subjects with stage 2 chronic kidney disease, and 30 subjects with stage 3 chronic kidney disease.ConclusionA nanotechnology-based PoC device for quantitative measurement of HbA1c, hemoglobin, serum albumin, microalbuminuria, and the urine albumin-to-creatinine ratio was developed for detection of early DKD and showed excellent correlation between the device and laboratory results. This device has the potential for early detection of DM and/or DKD, especially in remote communities in underserved areas of the world where prevalence of diabetes is rapidly increasing.

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“…A non-enzymatic electrochemical technique for creatinine sensing was presented by exploiting the iron binding property of creatinine. Disposable carbon printed electrodes layered with FeCl 3 coated cotton fiber membranes were used to sense creatinine from 10 to 245 mg/dL, on clinical urine samples [ 45 ].…”

Section: Creatininementioning

confidence: 99%

Miniaturized Bio-and Chemical-Sensors for Point-of-Care Monitoring of Chronic Kidney Diseases

Tricoli

Neri

2018

Sensors

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This review reports the latest achievements in point-of-care (POC) sensor technologies for the monitoring of ammonia, creatinine and urea in patients suffering of chronic kidney diseases (CKDs). Abnormal levels of these nitrogen biomarkers are found in the physiological fluids, such as blood, urine and sweat, of CKD patients. Delocalized at-home monitoring of CKD biomarkers via integration of miniaturized, portable, and low cost chemical- and bio-sensors in POC devices, is an emerging approach to improve patients’ health monitoring and life quality. The successful monitoring of CKD biomarkers, performed on the different body fluids by means of sensors having strict requirements in term of size, cost, large-scale production capacity, response time and simple operation procedures for use in POC devices, is reported and discussed.

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Creatinine-Iron Complex and Its Use in Electrochemical Measurement of Urine Creatinine

Cited by 38 publications

References 26 publications

New Disposable Electrochemical Paper‐based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample

New Disposable Electrochemical Paper‐based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample

Application of a Nanotechnology-Based, Point-of-Care Diagnostic Device in Diabetic Kidney Disease

Miniaturized Bio-and Chemical-Sensors for Point-of-Care Monitoring of Chronic Kidney Diseases

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