Creation of a New Synthetic Medium for Culturing Helicobacter Pylori

Вартанова, Н. О.; Arzumanyan, V. G.; Serdyuk, O. A.; Temper, R M

doi:10.1007/s10517-005-0350-y

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Culture remains a reference method that appears to be feasible in a clinical setting [43]. A new synthetic medium, replacing peptones and blood with hemoglobin, glutamine, and trace aminoacids in brucella medium, was proposed in Russia but no comparison with standard media was provided [44].…”

Section: Invasive Testsmentioning

confidence: 99%

Diagnosis of Helicobacter pylori Infection

Dzierżanowska-Fangrat

et al. 2006

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A growing interest in non-invasive tests for the detection of Helicobacter pylori has been observed recently, reflecting a large number of studies published this year. New tests have been validated, and the old ones have been used in different clinical situations or for different purposes. Stool antigen tests have been extensively evaluated in pre- and post-treatment settings both in adults and children, and the urea breath test has been studied as a predictor of bacterial load, severity of gastric inflammation, and response to eradication treatment. Several studies have also explored the usefulness of some serologic markers as indicators of the gastric mucosa status. With regard to invasive tests, molecular methods are being used more and more, but the breakthrough this year was the direct in vivo observation of H. pylori during endoscopy.

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Section: Invasive Testsmentioning

confidence: 99%

Diagnosis of Helicobacter pylori Infection

Dzierżanowska-Fangrat

et al. 2006

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“…H. pylori has traditionally been grown on complex media, usually supplemented with either 5 to 10% blood or serum. Over the last 15 years, however, steps have been taken to develop defined media in order to study the nutritional and metabolic requirements of H. pylori (1,7,29,32,38,43,45). Most recently, the absolute amino acid requirements for the growth of H. pylori, as well as the requirements of other nutrients (such as trace metals) for H. pylori and other Helicobacter species, were determined (42).…”

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confidence: 99%

Peptide Transport in Helicobacter pylori : Roles of Dpp and Opp Systems and Evidence for Additional Peptide Transporters

Weinberg

Maier

2007

J Bacteriol

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Despite research into the nutritional requirements of Helicobacter pylori, little is known regarding its use of complex substrates, such as peptides. Analysis of genome sequences revealed putative ABC-type transporter genes for dipeptide (dppABCDF) and oligopeptide (oppABCD) transport. Genes from each system were PCR amplified, cloned, and disrupted by cassette insertion either individually (dppA, dppB, dppC, oppA, oppB, and oppC) or to create double mutants (dppA oppA, dppB oppB, dppB dppC, and oppB oppC). Peptide-utilizing abilities of the strains were assessed by monitoring growth in a chemically defined medium where the only source of the essential amino acid isoleucine was from peptides of various lengths (two to nine amino acids long). The dipeptide system mutants lacked the ability to use certain dipeptides, hexapeptides, and nonapeptides. However, these mutants retained some ability to grow with other dipeptides, tripeptides, and tetrapeptides. Of the oligopeptide mutants, only the oppB strain differed significantly from the wild type. This strain showed a wild-type phenotype for growth with longer peptides (hexa-and nonapeptides) while having a decreased ability to utilize di-, tri-, and tetrapeptides. The dppA oppA and dppB oppB mutants showed similar phenotypes to those of the dppA and dppB mutants, respectively. Peptide digestion by metalloproteases was ruled out as the cause for residual peptide transport by growing mutant strains in the presence of either EDTA or EGTA. Degradation products associated with a fluorescein isothiocyanate-labeled hexapeptide (plus cells) were minimal. An as yet unidentified peptide transport system(s) in H. pylori is proposed to be responsible for the residual transport.Helicobacter pylori is a gram-negative bacterium that is the causative agent of a number of stomach-related diseases, including peptic ulcer disease and certain gastric cancers (3). H. pylori has traditionally been grown on complex media, usually supplemented with either 5 to 10% blood or serum. Over the last 15 years, however, steps have been taken to develop defined media in order to study the nutritional and metabolic requirements of H. pylori (1,7,29,32,38,43,45). Most recently, the absolute amino acid requirements for the growth of H. pylori, as well as the requirements of other nutrients (such as trace metals) for H. pylori and other Helicobacter species, were determined (42). Although it has been shown to grow on glucose (26-28), H. pylori does not have the enzymatic machinery to break down more complex carbohydrates (11) and is thus limited in its ability to utilize carbohydrates as carbon sources. The bacterium can incorporate some simple amino acids as a carbon source, but the same studies showed that H. pylori requires a number of amino acids, including methionine, alanine, histidine, isoleucine, leucine, and valine, for protein synthesis (1, 38). These findings are in agreement with the genomic information available (11). On the other hand, there have not been studies concerning H. pylori's a...

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Creation of a New Synthetic Medium for Culturing Helicobacter Pylori

Cited by 2 publications

References 7 publications

Diagnosis of Helicobacter pylori Infection

Diagnosis of Helicobacter pylori Infection

Peptide Transport in Helicobacter pylori : Roles of Dpp and Opp Systems and Evidence for Additional Peptide Transporters

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